FlyBase Allele Report: Zzzz\CAG[Q48.UAS.Tag:MYC,Tag:FLAG]

General Information

Symbol

Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG}

Species

a. artificial

Name

FlyBase ID

FBal0127243

Feature type

allele

Associated gene

Zzzz\CAG

Associated Insertion(s)

Carried in Construct

P{UAS-Q48.myc.flag}

Also Known As

P{UAS-Q48.myc/flag}

Transgenic product class

trinucleotide_repeat_expansion

(Steffan et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

trinucleotide_repeat_expansion

(Steffan et al., 2001)

Mutagen

in vitro construct

(Steffan et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Q48.myc.flag}

Cytology

Description

UAS sequences drive expression of 48 Glutamines. This is tagged with Tag:MYC and Tag:FLAG.

(Steffan et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Underwood et al., 2010, Steffan et al., 2001)

model of Huntington's disease

CEA

(Jimenez-Sanchez et al., 2015, Lievens et al., 2005)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of neurodegenerative disease

is exacerbated by Sod1^UAS.cAa

(Underwood et al., 2010)

is exacerbated by Sod2^UAS.cMa

(Underwood et al., 2010)

is ameliorated by CalpA^KK104532

(Menzies et al., 2015)

model of Huntington's disease

is ameliorated by QC^GD6767

(Jimenez-Sanchez et al., 2015)

is ameliorated by QC^KK109667

(Jimenez-Sanchez et al., 2015)

is ameliorated by isoQC^KK108983

(Jimenez-Sanchez et al., 2015)

is ameliorated by Hsap\HTT^{Su3.Scer\UAS.T:Zzzz\CAG,T:Hsap\MYC}

(Kazantsev et al., 2002)

is ameliorated by Zzzz\CAG^{Su3.UAS.Tag:MYC}

(Kazantsev et al., 2002)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Jimenez-Sanchez et al., 2015, Menzies et al., 2015, Underwood et al., 2010)

eye photoreceptor cell & neuron, with Scer\GAL4^elav.PLu

(Kazantsev et al., 2002)

photoreceptor, with Scer\GAL4^GMR.PF

(Scappini et al., 2007)

rhabdomere, with Scer\GAL4^elav.PLu

(Steffan et al., 2001)

rhabdomere | adult stage, with Scer\GAL4^elav-C155

(Chongtham and Agrawal, 2016)

Detailed Description

Statement

Reference

Expression of Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 results in photoreceptor neurodegeneration in the adult eye at 7 days post-eclosion and reduced crawling ability in third instar larvae.

(Chongtham and Agrawal, 2016)

Expression of Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF causes eye degeneration.

(Jimenez-Sanchez et al., 2015)

Expression of Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF causes severe eye degeneration with loss of pigmentation in the eye and formation of black necrotic-like spots.

(Menzies et al., 2015)

Expression of Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} in photoreceptors under the control of Scer\GAL4^GMR.PF results in electroretinogram depolarisation amplitudes that are 55% lower than controls.

(Scappini et al., 2007)

Flies expressing Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^Eaat1.PR have a shortened lifespan compared to wild type and are found to be lethargic a few days before dying.

(Lievens et al., 2005)

Expression of Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PLu results in loss of 1-2 photoreceptor neurons per ommatidium and some gaps in the eye tissue.

(Kazantsev et al., 2002)

Expression of Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} driven by Scer\GAL4^elav.PLu leads to degeneration of rhabdomeres and partial lethality. Suberoylanilide hydroxamic acid (SAHA) suppresses the lethality and reduces the level of degeneration seen in the eye to wild-type levels.

(Steffan et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, enhanceable by Sod1^UAS.cAa/Scer\GAL4^GMR.PF

(Underwood et al., 2010)

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, enhanceable by Sod2^UAS.cMa/Scer\GAL4^GMR.PF

(Underwood et al., 2010)

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has abnormal neurophysiology phenotype, enhanceable by Dap160^UAS.cKa, Scer\GAL4^GMR.PF

(Scappini et al., 2007)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has abnormal neurophysiology phenotype, non-enhanceable by Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF

(Scappini et al., 2007)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, suppressible by QC^GD6767, Scer\GAL4^GMR.PF

(Jimenez-Sanchez et al., 2015)

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, suppressible by QC^KK109667, Scer\GAL4^GMR.PF

(Jimenez-Sanchez et al., 2015)

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, suppressible by isoQC^KK108983/Scer\GAL4^GMR.PF

(Jimenez-Sanchez et al., 2015)

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, suppressible | partially by CalpA^KK104532, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

Scer\GAL4^elav.PLu, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has partially lethal - majority die phenotype, suppressible | partially by Zzzz\CAG^{Su3.UAS.Tag:MYC}/Scer\GAL4^elav.PLu

(Kazantsev et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, enhanceable by Sod1^UAS.cAa/Scer\GAL4^GMR.PF

(Underwood et al., 2010)

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, enhanceable by Sod2^UAS.cMa/Scer\GAL4^GMR.PF

(Underwood et al., 2010)

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has photoreceptor phenotype, enhanceable by Dap160^UAS.cKa, Scer\GAL4^GMR.PF

(Scappini et al., 2007)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has photoreceptor phenotype, non-enhanceable by Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF

(Scappini et al., 2007)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, suppressible by QC^GD6767, Scer\GAL4^GMR.PF

(Jimenez-Sanchez et al., 2015)

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, suppressible by QC^KK109667, Scer\GAL4^GMR.PF

(Jimenez-Sanchez et al., 2015)

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, suppressible by isoQC^KK108983/Scer\GAL4^GMR.PF

(Jimenez-Sanchez et al., 2015)

Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, suppressible | partially by CalpA^KK104532, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

Scer\GAL4^elav.PLu, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG} has eye photoreceptor cell & neuron phenotype, suppressible by Zzzz\CAG^{Su3.UAS.Tag:MYC}/Scer\GAL4^elav.PLu

(Kazantsev et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of CalpA^KK104532 leads to a significant reduction in the number of flies with black necrotic-like spots in the eye, in both males and females expressing Scer\GAL4^GMR.PF>Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}.

(Menzies et al., 2015)

Co-expression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} does not enhance the reduction in electroretinogram depolarisation amplitude seen when Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} is expressed in photoreceptors under the control of Scer\GAL4^GMR.PF.

Co-expression of Dap160^Scer\UAS.cKa enhances the reduction in electroretinogram depolarisation amplitude seen when Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} is expressed in photoreceptors under the control of Scer\GAL4^GMR.PF.

(Scappini et al., 2007)

Co-expression of Hsap\HD^{Su3.Scer\UAS.T:Zzzz\CAG,T:Hsap\MYC} significantly suppresses the loss of photoreceptor neurons seen in flies expressing Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PLu.

(Kazantsev et al., 2002)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51674

w^* P{UAS-Q48.myc.flag}42

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}

Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections