FlyBase Allele Report: Scer\GAL4[Eaat1.PR]

FB2024_04 , released June 25, 2024

FB2024_04 , released June 25, 2024

Allele: Scer\GAL4^Eaat1.PR

General Information

Symbol

Scer\GAL4^Eaat1.PR

Species

S. cerevisiae

Name

Excitatory amino acid transporter 1 promoter construct of Rival

FlyBase ID

FBal0159810

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{Eaat1-GAL4.R}

Also Known As

eaat1-Gal4, dEAAT1-GAL4, Eaat-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rival et al., 2004, Rival et al., 2004)

Progenitor genotype

Carried in construct

P{Eaat1-GAL4.R}

Cytology

Description

An 8.5kb genomic segment that contains 4kb of the Eaat1 upstream region, the first exon and the first intron of Eaat1 fused to sequence encoding a GAL4 driver.

(Rival et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 16

medial dorsal subperineurial glial cell

Comment: in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)

embryonic/larval neuron

Comment: in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)

medial-most cell body glial cell

(Beckervordersandforth et al., 2008)

medial cell body glial cell

(Beckervordersandforth et al., 2008)

lateral cell body glial cell

(Beckervordersandforth et al., 2008)

first instar larval stage

larval ventral nerve cord | subset

• cell projection membrane

(Stacey et al., 2010)

embryonic longitudinal glial cell | subset

(Stacey et al., 2010)

Additional Information

Statement

Reference

Drives expression in predominantly in reticular neuropil associated glial cells (astrocytes), with some expression in cortex and ensheathing glial cells.

(FlyBase Curators, 2017)

ScerGAL4^Eaat1.PR drives expression in the same glial cells as EcollexA^GMR75H11, although slightly more in number.

(Aw et al., 2017)

ScerGAL4^Eaat1.PR labels neurons that have their cell bodies in the anterior and posterior of the medulla cortex. They send axons into the lamina neuropil via the optic chiasm, and some fibers are also seen in the medulla neuropil.

(Hamanaka and Meinertzhagen, 2010)

ScerGAL4^Eaat1.PR transcript is expressed in almost all of the the longitudinal (interface) glial cells where it is co-expressed AvicGFP^{CG31235.T:nls-tra}. The majority of the cells labeled with ScerGAL4^Eaat1.PR co-express pros, which marks the six anterior longitudinal glial cells. ScerGAL4^Eaat1.PR is expressed in the glial membrane projections in the ventral nerve cord, adjacent to VGlut puncta.

(Stacey et al., 2010)

ScerGAL4^Eaat1.PR drives expression in the glial processes that wrap the motor axons in the adult neuromuscular junction.

(Rival et al., 2006)

Marker for

Reflects expression of

(Hamanaka and Meinertzhagen, 2010, Stacey et al., 2010, Doherty et al., 2009, Beckervordersandforth et al., 2008, Rival et al., 2006, Lievens et al., 2005)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Eaat1^RNAi.UAS

(Rival et al., 2006, Rival et al., 2004)

abnormal locomotor behavior, with Eaat1^RNAi.UAS

(Rival et al., 2004)

abnormal locomotor behavior | adult stage, with Scer\GAL80^elav.PU, nkt^GD8239

(Sengupta et al., 2019)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL80^ts.αTub84B, shi^1.UAS

(Ng et al., 2011)

abnormal neurophysiology, with Eaat1^RNAi.UAS

(Danjo et al., 2011, Rival et al., 2006)

abnormal touch response, with Eaat1^RNAi.UAS

(Rival et al., 2004)

decreased rate of movement | adult stage, with Hsap\HTT^Q93.ex1.UAS

(Lievens et al., 2005)

decreased rate of movement | adult stage, with Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG}

(Lievens et al., 2005)

lethal, with Gug^66QC.UAS

(Nisoli et al., 2010)

lethal, with Gug^75QN.UAS

(Nisoli et al., 2010)

lethal, with Hsap\ATN1^65Q.UAS

(Nisoli et al., 2010)

lethal, with Hsap\ATN1^N917.65Q.UAS

(Nisoli et al., 2010)

short lived, with Hsap\HTT^Q93.ex1.UAS

(Dupont et al., 2012, Besson et al., 2010, Liévens et al., 2008, Lievens et al., 2005)

short lived, with rl^Sem.C.UAS

(Liévens et al., 2008)

short lived, with Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG}

(Lievens et al., 2005)

Phenotype Manifest In

adult neuromuscular junction, with Eaat1^RNAi.UAS

(Danjo et al., 2011)

dopaminergic neuron, with Eaat1^RNAi.UAS

(Rival et al., 2004)

neuromuscular junction, with Eaat1^RNAi.UAS

(Rival et al., 2006)

neuropil, with Eaat1^RNAi.UAS

(Rival et al., 2004)

synapse, with Eaat1^RNAi.UAS

(Danjo et al., 2011)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, non-enhanceable by Bmcp^BG02446/Bmcp[+]

(Besson et al., 2010)

Suppressed by

Statement

Reference

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, suppressible | partially by arm³/arm[+]

(Dupont et al., 2012)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, suppressible | partially by arm^G0192/arm[+]

(Dupont et al., 2012)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, suppressible | partially by pan[+]/pan³

(Dupont et al., 2012)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, suppressible | partially by pan[+]/pan²

(Dupont et al., 2012)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, suppressible | partially by Axn^UAS.cUa, Scer\GAL4^Eaat1.PR

(Dupont et al., 2012)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, suppressible by Bmcp^UAS.cBa, Scer\GAL4^Eaat1.PR

(Besson et al., 2010)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, suppressible by Hsap\UCP2^UAS.cFa, Scer\GAL4^Eaat1.PR

(Besson et al., 2010)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, suppressible | partially by Hsap\HSPA1L^UAS.cWa, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, suppressible | partially by DnaJ-1^UAS.cKa, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, suppressible | partially by mrj^UAS.cFa, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

Eaat1^RNAi.UAS, Scer\GAL4^Eaat1.PR has abnormal neurophysiology phenotype, suppressible by Hsap\SLC1A2^UAS.cRa, Scer\GAL4^Eaat1.PR

(Rival et al., 2006)

Eaat1^RNAi.UAS, Scer\GAL4^Eaat1.PR has abnormal touch response phenotype, suppressible by Hsap\SLC1A2^UAS.cRa, Scer\GAL4^Eaat1.PR

(Rival et al., 2004)

Eaat1^RNAi.UAS, Scer\GAL4^Eaat1.PR has abnormal flight phenotype, suppressible by Hsap\SLC1A2^UAS.cRa, Scer\GAL4^Eaat1.PR

(Rival et al., 2004)

Eaat1^RNAi.UAS, Scer\GAL4^Eaat1.PR has abnormal locomotor behavior phenotype, suppressible by Hsap\SLC1A2^UAS.cRa, Scer\GAL4^Eaat1.PR

(Rival et al., 2004)

NOT suppressed by

Statement

Reference

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, non-suppressible by shg[+]/shg^k03401

(Dupont et al., 2012)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, non-suppressible by Bmcp^BG02446/Bmcp[+]

(Besson et al., 2010)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, non-suppressible by Sod2^+t9/Cat^{Tag:Mito(Oat)}

(Besson et al., 2010)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, non-suppressible by Akt^UAS.Exel, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, non-suppressible by rl^Sem.C.UAS, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR has short lived phenotype, non-suppressible by Tpr2^UAS.cKa, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

Suppressor of

Statement

Reference

Axn^UAS.cUa, Scer\GAL4^Eaat1.PR is a suppressor | partially of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Dupont et al., 2012)

Bmcp^UAS.cBa, Scer\GAL4^Eaat1.PR is a suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Besson et al., 2010)

Hsap\UCP2^UAS.cFa, Scer\GAL4^Eaat1.PR is a suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Besson et al., 2010)

Hsap\HSPA1L^UAS.cWa, Scer\GAL4^Eaat1.PR is a suppressor | partially of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

DnaJ-1^UAS.cKa, Scer\GAL4^Eaat1.PR is a suppressor | partially of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

mrj^UAS.cFa, Scer\GAL4^Eaat1.PR is a suppressor | partially of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

Hsap\SLC1A2^UAS.cRa, Scer\GAL4^Eaat1.PR is a suppressor of abnormal neurophysiology phenotype of Eaat1^RNAi.UAS, Scer\GAL4^Eaat1.PR

(Rival et al., 2006)

Hsap\SLC1A2^UAS.cRa, Scer\GAL4^Eaat1.PR is a suppressor of abnormal locomotor behavior phenotype of Eaat1^RNAi.UAS, Scer\GAL4^Eaat1.PR

(Rival et al., 2004)

Hsap\SLC1A2^UAS.cRa, Scer\GAL4^Eaat1.PR is a suppressor of abnormal touch response phenotype of Eaat1^RNAi.UAS, Scer\GAL4^Eaat1.PR

(Rival et al., 2004)

Hsap\SLC1A2^UAS.cRa, Scer\GAL4^Eaat1.PR is a suppressor of abnormal flight phenotype of Eaat1^RNAi.UAS, Scer\GAL4^Eaat1.PR

(Rival et al., 2004)

NOT Suppressor of

Statement

Reference

Akt^UAS.Exel, Scer\GAL4^Eaat1.PR is a non-suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

rl^Sem.C.UAS, Scer\GAL4^Eaat1.PR is a non-suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

Tpr2^UAS.cKa, Scer\GAL4^Eaat1.PR is a non-suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Eaat1^RNAi.UAS, Scer\GAL4^Eaat1.PR has neuromuscular junction phenotype, suppressible by Hsap\SLC1A2^UAS.cRa, Scer\GAL4^Eaat1.PR

(Rival et al., 2006)

Suppressor of

Statement

Reference

Hsap\SLC1A2^UAS.cRa, Scer\GAL4^Eaat1.PR is a suppressor of neuromuscular junction phenotype of Eaat1^RNAi.UAS, Scer\GAL4^Eaat1.PR

(Rival et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

8849

w^*; P{Eaat1-GAL4.R}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^Eaat1.PR

Name Synonyms

Excitatory amino acid transporter 1 promoter construct of Rival

(Rival et al., 2004, Rival et al., 2004)

Secondary FlyBase IDs

References (41)

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