FlyBase Allele Report: Dmel\Gr5a[ΔEP5]

General Information

Symbol

Dmel\Gr5a^ΔEP5

Species

D. melanogaster

Name

FlyBase ID

FBal0127245

Feature type

allele

Associated gene

Dmel\Gr5a

Associated Insertion(s)

Carried in Construct

Also Known As

ΔGr5a, ΔEP(X)-5

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Ueno et al., 2001)

Progenitor genotype

P{EP}Tre1^EP496

(Ueno et al., 2001)

Caused by aberration

Df(1)Tre^ΔEP5

Cytology

Description

Proximal breakpoint lies within the first Gr5a intron, which lies 3' to the codon specifying Arg 36. The translation initiation codon of Gr5a is removed. Distal breakpoint falls in the first intron of Tre1.

(Dahanukar et al., 2001)

Imprecise excision of the progenitor insertion, resulting in a 2.5kb deletion of genomic sequences uncovering 0.9kb towards Gr5a (removing the promoter, 5' leader sequences and sequences to 133bp downstream of the start codon) and 1.6b towards Tre1 (removing the promoter, exon 1 and part of intron 1).

(Ueno et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:5,678,368..5,680,859 [+]

Comment:

2492 bp deletion associated with the imprecise excision of P{EP}Tre1^EP496 (GB:AB066611).

(Ueno et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

AB066611

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal feeding behavior

(Wisotsky et al., 2011)

abnormal neurophysiology

(Dahanukar et al., 2001)

abnormal neurophysiology | adult stage

(Dahanukar et al., 2007)

abnormal proboscis extension reflex

(Dahanukar et al., 2007)

abnormal taste perception

(Freeman et al., 2014, Dahanukar et al., 2001, Ueno et al., 2001)

abnormal taste perception | recessive

(Jiao et al., 2008)

Phenotype Manifest In

large labellar taste bristle

(Dahanukar et al., 2007)

Detailed Description

Statement

Reference

Gr5a^ΔEP5 mutants exhibit a statistically significant reduction in the response to trehalose, melezitose, m-glucoside and glucose. However, responses to sucrose, maltose, maltotriose, fructose and glycerol are unaffected.

(Freeman et al., 2014)

Gr5a^ΔEP5 mutant flies display a preference for a 30% beer concentration, as in wild-type, against 5mM sucrose.

Gr64d^MB03533 flies display wild-type levels of preference for yeast extract, fermented grape and banana.

(Wisotsky et al., 2011)

In contrast to wild-type flies, homozygous Gr5a^ΔEP5 mutant flies produce action potentials in response to only high concentrations of trehalose.

(Jiao et al., 2008)

The mean electrophysiological response of mutant L-type labellar sensilla to trehalose, m-α-glucoside, glucose or melezitose is reduced compared to controls.

The mean proboscis extension response of mutant flies to glucose, melezitose and m-α-glucoside is reduced compared to controls, but the response to fructose, stachyose, maltotriose, maltose or sucrose is not affected.

(Dahanukar et al., 2007)

Electrophysiological response to sugars in individual labellar taste hairs of the large and medium class shows normal response to sucrose but drastically reduced response to trehalose, in comparison with parental P{EP}Tre1^EP496 strain. In a two choice preference test with sucrose and trehalose, mutants show dramatically reduced response to trehalose, when compared to the parental P{EP}Tre1^EP496 strain.

(Dahanukar et al., 2001)

Flies show reduced sensitivity to trehalose in a feeding preference test.

(Ueno et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Gr5a⁰¹

(Ueno et al., 2001)

Rescued by

Gr5a^ΔEP5 is rescued by Gr5a^UAS.cDa/Scer\GAL4^Gr5a.8.5

(Dahanukar et al., 2007)

Gr5a^ΔEP5 is rescued by Gr5a^+t10

(Dahanukar et al., 2001)

Not rescued by

Gr5a^ΔEP5 is not rescued by Gr5a^STOP

(Dahanukar et al., 2001)

Comments

Expression of Gr5a^Scer\UAS.cDa under the control of Scer\GAL4^Gr5a.8.5 rescues the reduction in the electrophysiological response to the sugars trehalose, m-α-glucoside, glucose and melezitose that is seen in Gr5a^ΔEP5 flies.

Expression of Gr5a^Scer\UAS.cDa under the control of Scer\GAL4^Gr5a.8.5 increases the mean proboscis extension response to all sugars whose responses are reduced in Gr5a^ΔEP5 flies.

(Dahanukar et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AB066611

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Gr5a^EP-5

(Landayan et al., 2018)

Gr5a^Δ5

(Shrestha et al., 2023)

Gr5a^ΔEP5

(Wisotsky et al., 2011)

Tre^ΔEP5

ΔEP(X)-5

(Dahanukar et al., 2007, Dahanukar et al., 2001)

ΔGr5a

(Wang et al., 2016, Freeman et al., 2014, Jiao et al., 2008, Dahanukar et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (10)

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