FlyBase Allele Report: Scer\GAL4[Gr5a.8.5]

ScerGAL4^Gr5a.8.5 drives expression in all sweet taste receptor neurons in the labellar taste hairs, and in about 9 segmental tarsal sugar-sensing chemosensory neurons in the prothoracic leg that project to the prothoracic ganglion.

(Thoma et al., 2016)

ScerGAL4^Gr5a.8.5 drives expression in adult abdominal multidendritic neurons with with dorsal processes extending along the left-right axis, as well as in multidendritic neurons with lateral processes extending anteroposteriorly; these major processes have several protruding dendrites. Additionally, expression is driven in laterally localized multidendritic neurons with extensive arborization. Expression is also observed in projections to the abdominal ganglion.

(Park and Kwon, 2011)

ScerGAL4^Gr5a.8.5 is expressed in the sugar-sensitive neuron of a subset of the short labellar taste bristles.

(Weiss et al., 2011)

ScerGAL4^Gr5a.8.5 is strongly expressed in the sugar-sensitive neuron of all long, intermediate and short taste sensilla of the labellum.

(Weiss et al., 2011)

ScerGAL4^Gr5a.8.5 is expressed in all the sugar-sensitive receptor neurons of the gustatory sensilla of the labellum.

(Weiss et al., 2011)

ScerGAL4^Tre.8.5.cCa expresses in Tre (Gustatory receptor Gr5a) neurons.

(Yang et al., 2008)

ScerGAL4^Tre.8.5.cCa expresses in approximately 30 neurons per labial palp, in one neuron in most sensilla.

(Wang et al., 2004)

Marker for

sugar-sensing neuron of taste bristle

(Mitri et al., 2009)

Reflects expression of

Gr5a

(Sánchez-Alcañiz et al., 2018, Thoma et al., 2016, Clowney et al., 2015, Ling et al., 2014, Park and Kwon, 2011, Weiss et al., 2011, Weiss et al., 2011, Yang et al., 2008, Wang et al., 2004)

Reporter construct used in assay

Human Disease Associations

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Disease

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Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Thoma et al., 2016)

abnormal feeding behavior | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Kang et al., 2012)

abnormal locomotor behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Thoma et al., 2016)

abnormal neurophysiology | adult stage, with Cbeta\DT^A-I.UAS

(Dweck et al., 2022)

abnormal neurophysiology | adult stage, with TrpA1^UAS.(A).cKa

(Kang et al., 2012)

abnormal neurophysiology | adult stage, with TrpA1^UAS.(B).cKa

(Kang et al., 2012)

abnormal oviposition | chemical conditional, with Hsap\KCNJ2^{IVS.10xUAS.EGFP}

(Wu et al., 2019)

abnormal proboscis extension reflex, with Hsap\KCNJ2^UAS.EGFP

(Thoma et al., 2016)

abnormal proboscis extension reflex, with OtopLa¹, OtopLa^E638A.UAS

(Mi et al., 2021)

abnormal proboscis extension reflex | conditional, with Crei\ChR2^T159C.UAS

(Dawydow et al., 2014)

abnormal proboscis extension reflex | conditional, with Crei\ChR2^XXL.UAS

(Dawydow et al., 2014)

abnormal proboscis extension reflex | heat sensitive, with TrpA1^UAS.(B).cKa

(Kain and Dahanukar, 2015)

abnormal tarsal response | chemical conditional, with DopEcR^KK111211

(Inagaki et al., 2012)

abnormal tarsal response | conditional, with Crei\ChR2^{C128T.UAS.EYFP}

(Inagaki et al., 2012)

abnormal tarsal response | nutrition conditional, with DopEcR^KK111211

(Inagaki et al., 2012)

abnormal tarsal response | nutrition conditional, with DopEcR^UAS.cIa

(Inagaki et al., 2012)

abnormal taste perception, with Cbeta\DT^A-I.UAS

(Wang et al., 2004)

abnormal taste perception, with Mmmm\TRPV1^E600K.UAS

(Marella et al., 2006)

abnormal taste perception, with OtopLa¹, OtopLa^E638A.UAS

(Mi et al., 2021)

viable, with mys^RNAi.Sym.UAS

(Bhandari et al., 2006)

Phenotype Manifest In

large labellar taste bristle, with Cbeta\DT^A-I.UAS

(Dweck et al., 2022)

large labellar taste bristle, with TrpA1^UAS.(A).cKa

(Kang et al., 2012)

Detailed Description

Statement

Reference

Inhibition (using Hsap\KCNJ2^{IVS.10xUAS.EGFP}) of Scer\GAL4^Gr5a.8.5 cells in adult females results in a significant increase in preference for softer 100 mM sucrose-containing, but not plain, agarose for egg laying, compared to controls.

(Wu et al., 2019)

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^Gr5a.8.5 neurons in adults does not affect wind-induced suppression of locomotion or basal walking speed, compared to controls.

(Tsubouchi et al., 2017)

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^Gr5a.8.5 cells in adults does not significantly affect preference for sucrose, compared to controls; there is a significant reduction in proboscis extension reflex probability in response to labellar, but not tarsal, sucrose, but no significant effect on short term appetitive (sucrose) or aversive olfactory memory, compared to controls; unlike controls, there is no significant sugar-induced suppression of angular or linear velocity. When inhibition is restricted to a smaller set of cells (using Scer\FLP1^{FLPo.oc.Tag:NLS(SV40-largeT)} and Scer\GAL80^αTub84B.PG) significant sugar-induced suppression of angular and linear velocity is seen, similar to controls.

(Thoma et al., 2016)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 32[o]C) of Scer\GAL4^Gr5a.8.5 neurons induces a high frequency of proboscis extension, compared to controls.

(Kain and Dahanukar, 2015)

Ablation of Scer\GAL4^Gr5a.8.5-expressing neurons does not affect sensitivity to the gustatory sex pheromone (S,Z,Z)-CH503: as in wild type, courtship defects are seen in these flies in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Inhibition (using Ork1^{Δ-C.UAS.EGFP}) of Scer\GAL4^Gr5a.8.5 cells in adults given a choice between sucrose solution and water does not affect water or sucrose consumption, compared to controls.

(Sun et al., 2014)

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