FlyBase Allele Report: Dmel\Doa[EP3080]

General Information

Symbol

Dmel\Doa^EP3080

Species

D. melanogaster

Name

FlyBase ID

FBal0131259

Feature type

allele

Associated gene

Dmel\Doa

Associated Insertion(s)

P{EP}Doa^EP3080

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-, FlyBase, 1996-)

Progenitor genotype

Associated Insertion(s)

P{EP}Doa^EP3080

Cytology

Description

The P{EP} element is inserted 45 nucleotides 5' to the first non-coding exon of Doa (55kDa) and 780 nucleotides 5' to its initiating AUG.

(Kpebe and Rabinow, 2008)

The P{EP}Doa^EP3080 insertion maps within both Doa and CG33203 (overlapping genes on opposite strands).

(FlyBase, 1996-)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:28,888,981..28,888,981 [+]

(Kpebe and Rabinow, 2008, Gene Disruption Project members, 2001-, FlyBase, 1996-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal | male limited, with Scer\GAL4^Lsp2.PH

(Kpebe and Rabinow, 2008)

some die during embryonic stage | male limited, with Scer\GAL4^Lsp2.PH

(Kpebe and Rabinow, 2008)

visible | adult stage, with Scer\GAL4^da.G32

(Kpebe and Rabinow, 2008)

Phenotype Manifest In

eye, with Scer\GAL4^da.G32

(Kpebe and Rabinow, 2008)

ommatidium, with Scer\GAL4^GMR.PF

(Kpebe and Rabinow, 2008)

pigment cell | increased number, with Scer\GAL4^GMR.PF

(Kpebe and Rabinow, 2008)

rhabdomere | increased number, with Scer\GAL4^GMR.PF

(Kpebe and Rabinow, 2008)

wing, with Scer\GAL4^da.G32

(Kpebe and Rabinow, 2008)

wing vein | ectopic, with Scer\GAL4^da.G32

(Kpebe and Rabinow, 2008)

Detailed Description

Statement

Reference

Expression of Doa^EP3080 under the control of Scer\GAL4^da.G32 results in slight eye roughness and occasional minor ommatidial disorganisation. Ectopic wing vein formation is seen in the anterior compartment of the wing blade between L1 and L2.

Expression of Doa^EP3080 under the control of Scer\GAL4^GMR.PF results in substantial ommatidial fusion and disorganisation. Small holes are seen in the surface of the ommatidia at high magnification. Supernumerary pigment cells and rhabdomeres are present.

Expression of Doa^EP3080 under the control of Scer\GAL4^Lsp2.PH results in complete male lethality when Scer\GAL4^Lsp2.PH females are crossed with Doa^EP3080 males. In the reciprocal cross 18% of the progeny (instead of the 50% expected) are male. Lethality occurs at the embryonic stage. The males do not exhibit any abnormal phenotypes.

(Kpebe and Rabinow, 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Doa^EP3080, Scer\GAL4^Lsp2.PH has partially lethal | male limited phenotype, suppressible by tra^UAS.cFa, Scer\GAL4^Lsp2.PH

(Kpebe and Rabinow, 2008)

Doa^EP3080, Scer\GAL4^Lsp2.PH has some die during embryonic stage | male limited phenotype, suppressible by tra^UAS.cFa, Scer\GAL4^Lsp2.PH

(Kpebe and Rabinow, 2008)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of tra^Scer\UAS.cFa suppresses the male lethality seen when Doa^EP3080 is expressed under the control of Scer\GAL4^Lsp2.PH.

(Kpebe and Rabinow, 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Doa^EP3080

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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