FlyBase Allele Report: Dmel\AP-2σ[KG02457]

General Information

Symbol

Dmel\AP-2σ^KG02457

Species

D. melanogaster

Name

FlyBase ID

FBal0131822

Feature type

allele

Associated gene

Dmel\AP-2σ

Associated Insertion(s)

P{SUPor-P}AP-2σ^KG02457

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Choudhury et al., 2016)

amorphic allele - molecular evidence

(Choudhury et al., 2016)

loss of function allele

(Windler and Bilder, 2010)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}AP-2σ^KG02457

Cytology

Description

An insertion of P{SUPor-P} at codon 70 of AP-2σ.

(Windler and Bilder, 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:21,269,628..21,269,628 [-]

(Song and Lu, 2012, Windler and Bilder, 2010, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage (with AP-2σ^ang-7)

(Choudhury et al., 2016)

abnormal neuroanatomy | recessive | somatic clone

(Song and Lu, 2012)

abnormal neurophysiology | larval stage (with AP-2σ^ang-7)

(Choudhury et al., 2016)

lethal - all die before end of larval stage

(Choudhury et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Larval muscle 6/7 NMJs in AP-2σ^KG02457/AP-2σ^ang-7, transheterozygotes show significantly more and significantly smaller boutons, which exhibit a significant decrease in periodic microtubule loop structures (Futsch staining), and show a significant decrease in the vesicle pool, as compared to controls. Electrophysiology recordingS at the NMJ also show significant increases in mEJP frequency and amplitude, and significant decreases in EJP amplitude and quantal content, and show a significant decline in EJP amplitude readings upon high-frequency stimulation at 34[o]C conditions even after a 4min rest period, as compared to controls.

(Choudhury et al., 2016)

AP-2σ^KG02457 mutant MARCM clones contain supernumerary neuroblasts, compared with control clones.

(Song and Lu, 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

AP-2sigma[+]/AP-2σ^KG02457 is an enhancer of embryo | embryonic stage 5 phenotype of AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C}

(Lee and Harris, 2013)

AP-2sigma[+]/AP-2σ^KG02457 is an enhancer of furrow canal phenotype of AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C}

(Lee and Harris, 2013)

AP-2sigma[+]/AP-2σ^KG02457 is an enhancer of embryo | embryonic stage 4 phenotype of AP-2α^HMS00653, Scer\GAL4^{VP16.mat.αTub67C}

(Lee and Harris, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Maternal heterozygosity for AP-2σ^KG02457 enhances the furrow canal phenotype seen in embryos derived from mothers expressing AP-2α^HMS00653 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.

(Lee and Harris, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

AP-2σ^ang-5

(Choudhury et al., 2016)

AP-2σ^ang-2

(Choudhury et al., 2016)

AP-2σ^ang-7

(Choudhury et al., 2016)

Rescued by

AP-2σ^ang-7/AP-2σ^KG02457 is rescued by Scer\GAL4^Act5C.PU/AP-2σ^UAS.cCa

(Choudhury et al., 2016)

AP-2σ^KG02457 is rescued by Scer\GAL4^Act5C.PU/AP-2σ^UAS.cCa

(Choudhury et al., 2016)

Partially rescued by

AP-2σ^KG02457 is partially rescued by Scer\GAL4^elav-C155/AP-2σ^UAS.cCa

(Choudhury et al., 2016)

AP-2σ^ang-7/AP-2σ^KG02457 is partially rescued by Scer\GAL4^elav-C155/AP-2σ^UAS.cCa

(Choudhury et al., 2016)

Comments

The expression of AP-2σ^UAS.cCa under the control of Scer\GAL4^elav-C155 rescues the larval synaptic phenotypes but not the lethality observed in AP-2σ^KG02457 homozygotes and in AP-2σ^KG02457/AP-2σ^ang-7 transheterozygotes. Expression under the control of Scer\GAL4^Act5C.PU rescues both lethality and larval synaptic phenotypes in AP-2σ^KG02457 homozygotes and in AP-2σ^KG02457/AP-2σ^ang-7 transheterozygotes.

(Choudhury et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

64750

w^*; P{SUPor-P}AP-2σ^KG02457 P{neoFRT}82B/TM6B, Tb¹

13478

y¹; ry⁵⁰⁶ P{SUPor-P}AP-2σ^KG02457/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

AP-2σ

(Skwarek et al., 2014)

AP-2σ^KG02457

(Choudhury et al., 2016, Lin et al., 2015, Lee and Harris, 2013)

AP-2σ^KG

(Choudhury et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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