FlyBase Allele Report: Dmel\αSnap[UAS.cPa]

General Information

Symbol

Dmel\αSnap^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Pallanck

FlyBase ID

FBal0135792

Feature type

allele

Associated gene

Dmel\αSnap

Associated Insertion(s)

Carried in Construct

P{UAS-αSnap.P}

Also Known As

UAS-Snap

Key Links

Transgenic product class

cDNA

(Dunne et al., 2002)

wild_type

(Dunne et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Dunne et al., 2002)

wild_type

(Dunne et al., 2002)

Mutagen

in vitro construct

(Dunne et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-αSnap.P}

Cytology

Description

UAS sequences drive expression of a αSnap cDNA.

(Dunne et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

αSnap

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^RapGAP1-OK6

(Babcock et al., 2004)

lethal, with Scer\GAL4^elav-C155

(Babcock et al., 2004)

Phenotype Manifest In

bouton, with Scer\GAL4^RapGAP1-OK6

(Babcock et al., 2004)

embryonic/larval neuromuscular junction, with Scer\GAL4^RapGAP1-OK6

(Babcock et al., 2004)

eye, with Scer\GAL4^GMR.PF

(Babcock et al., 2004)

Golgi apparatus, with Scer\GAL4^hs.PB

(Dunne et al., 2002)

Golgi stack | ectopic, with Scer\GAL4^hs.PB

(Dunne et al., 2002)

wing, with Scer\GAL4^elav-C155

(Babcock et al., 2004)

Detailed Description

Statement

Reference

Expression of Snap^Scer\UAS.cPa under the control of Scer\GAL4^elav-C155 shows varying phenotypes according to the Snap^Scer\UAS.cPa insertion used. Larvae carrying the P{UAS-Snap.P}1 insertion show no difference in their neurotransmitter release parameters with respect to controls and survive to adulthood. In contrast, animals carrying the P{UAS-Snap.P}5 insertion show embryonic lethality when combined with the Scer\GAL4^elav-C155 driver.

Expression of Snap^Scer\UAS.cPa via the Scer\GAL4^OK6 driver allows survival with the P{UAS-Snap.P}5 insertion to the third stage of larval development, although these larvae are nearly paralyzed and display severe electrophysiological defects. The neuromuscular junctions show a dramatic reduction in axonal branching and bouton number.

Adults carrying the P{UAS-Snap.P}1 insertion, from which Snap is overexpressed under the control of Scer\GAL4^elav-C155, show wing inflation defects.

Expression of Snap^Scer\UAS.cPa from the P{UAS-Snap.P}1 insertion, driven by Scer\GAL4^GMR.PF, results in mildly rough eyes containing patches of necrotic tissue. Expression from the stronger P{UAS-Snap.P}5 insertion, with Scer\GAL4^GMR.PF, results in almost complete ablation of the eye.

(Babcock et al., 2004)

When Snap^Scer\UAS.cPa, Scer\GAL4^hs.PB mutant third instar larvae are subjected to heat treatment, their wing and leg imaginal discs show a phenotype in the morphology of the golgi area. The proportion of membrane that forms cisternae increases from about 30% (in heat shocked controls) to about 55%. About 68% of these are stacked compared top about 30% in controls. There is also an increase in the volume density of golgi membrane in these discs (about 2.7 times that seen in wild-type).

(Dunne et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

αSnap^UAS.cPa, Scer\GAL4^elav.PLu is a suppressor of abnormal neuroanatomy phenotype of Nsf2^EQ.UAS, Scer\GAL4^elav.PLu

(Laviolette et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

αSnap^UAS.cPa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Nsf2⁵⁵

(Babcock et al., 2004)

αSnap^UAS.cPa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Syx1A^Δ229

(Babcock et al., 2004)

Suppressed by

Statement

Reference

αSnap^UAS.cPa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by comt^UAS.cGa, Scer\GAL4^GMR.PF

(Babcock et al., 2004)

αSnap^UAS.cPa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Nsf2^UAS.cGa, Scer\GAL4^GMR.PF

(Babcock et al., 2004)

αSnap^UAS.cPa, Scer\GAL4^elav-C155 has wing phenotype, suppressible by comt^UAS.cGa, Scer\GAL4^elav-C155

(Babcock et al., 2004)

αSnap^UAS.cPa, Scer\GAL4^elav-C155 has wing phenotype, suppressible by Nsf2^UAS.cGa, Scer\GAL4^elav-C155

(Babcock et al., 2004)

Suppressor of

Statement

Reference

αSnap^UAS.cPa, Scer\GAL4^elav.PLu is a suppressor of embryonic/larval neuromuscular junction phenotype of Nsf2^EQ.UAS, Scer\GAL4^elav.PLu

(Laviolette et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Snap^Scer\UAS.cPa from the P{UAS-Snap.P}1 in a Nsf2⁵⁵ or Syx1A^Δ229 background results in an enhancement of the Snap^Scer\UAS.cPa phenotype in the eye, including increased necrosis around the perimeter of the compound eye and more dramatic furrowing at ommatidial junctions.

Co-expression of either comt^Scer\UAS.cGa or Nsf2^Scer\UAS.cGa with Snap^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF suppresses the eye phenotypes of the weaker P{UAS-Snap.P}1 insertion and partially suppresses the eye ablation phenotype of the stronger P{UAS-Snap.P}5 insertion. Furthermore, coexpression of either comt^Scer\UAS.cGa or Nsf2^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 completely suppresses the wing inflation defect of Scer\GAL4^elav-C155>Snap^Scer\UAS.cPa flies.

(Babcock et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Snap^Scer\UAS.cPa

αSnap^Scer\UAS.cPa

αSnap^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Pallanck

(Dunne et al., 2002)

Secondary FlyBase IDs

References (3)

Report Sections

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