FlyBase Allele Report: Dmel\Syx1A[Δ229]

Neuronal expression of Syx1A^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155 in Syx1A^3-69/Syx1A^Δ229 results in a dramatic reduction in the frequency of constitutive secretion to 8.8Hz from 24.9Hz. The average amplitude of EPSPs is also significantly reduced in Syx1A^3-69/Syx1A^Δ229 mutants expressing Syx1A^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155. This EPSP amplitude is similar to that in Syx1A^Scer\UAS.cBa/Scer\GAL4^elav-C155 flies, but significantly higher than wild-type.

(Lagow et al., 2007)

Homozygous mutant embryos fail to secrete cuticle, denticle belts and mouthhooks. Gut and ventral nerve cord development are grossly abnormal.

(Fergestad et al., 2001)

The oocyte accumulates vacuoles at later stages.

(Ruden et al., 2000)

Heterozygotes with Syx1A^3-69 show paralysis after a few seconds at 38^oC.

(Littleton et al., 1998)

Homozygotes exhibit complete blockade of all forms of neurotransmitter release. Incomplete compaction of the ventral nerve cord is observed. Late stage embryos exhibit incomplete fasciculation of the intersegmental and segmental nerve bundles and the longitudinal tracts. Also nonneural forms of secretion are impaired, yolk digestion in the gut is abnormal and several layers of the cuticle are missing. Syx1A^Δ229/Syx1A^15ts transheterozygotes produce progeny that exhibit reduced viability, rough eyes (disrupted ommatidial array, ommatidia may be missing, fused, improperly rotated or misaligned) and notching along the posterior margin of the wing. Germline clones produce viable oocytes but are rudimentary and do not produce eggs. Clones cannot be recovered in the eye as lack of Syx1A activity in cells of the developing eye causes lethality.

(Schulze and Bellen, 1996)

Paralysis and embryonic lethality results. Evoked synaptic transmission is completely eliminated at the NMJ. However the postsynaptic membrane responds normally to applied L-glutamate. The NMJs develop in the normal synaptic domains on muscles, and bouton morphology is normal at the light microscope level. Bouton number is reduced by 30-50%. Ultrastructure of embryonic synapses is largely indistinguishable from wild type. The percentage of vesicles that have contacted, or are close to, the presynaptic membrane is increased compared to wild type. At both NMJs and central synapses there is an unusual abundance of enlarged vesicles. Miniature excitatory junctional currents do not occur at the mature embryonic NMJ, though increased saline osmolarity can trigger mass exocytosis, as in wild type, demonstrating that the docked vesicles are mature and functional. Latrotoxin-induced fusion of synaptic vesicles fails.

(Broadie et al., 1995)

Embryos fail to secrete most or all cuticle, gut is morphologically abnormal and the yolk in the gut is undigested. Condensation of the CNS is incomplete. Subtle defects in axonal morphology and fasciculation, axon bundles of the intersegmental and segmental nerves appear thickened and irregular in shape. Neuromuscular architecture is relatively normal.

(Schulze et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Syx1A^Δ229/Syx1A[+] is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

Suppressor of

Statement

Reference

Syx1A^Δ229/Syx1A[+] is a suppressor of abnormal locomotor behavior | conditional phenotype of Hsap\HTT^128Q.FL.UAS, Scer\GAL4^C164

(Romero et al., 2008)

Syx1A^Δ229/Syx1A[+] is a suppressor of abnormal neurophysiology phenotype of Hsap\HTT^128Q.FL.UAS, Scer\GAL4^elav-C155

(Romero et al., 2008)

Syx1A^Δ229 is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^elav.PU

(Kaltenbach et al., 2007)

Syx1A^Δ229 is a suppressor of short lived phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^elav.PU

(Kaltenbach et al., 2007)

Syx1A^Δ229 is a suppressor of visible phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

Other

Statement

Reference

Syx1A^Δ229/Syx1A[+], Yif1^doc-1 has visible | adult stage phenotype

(Kandasamy et al., 2021)

Syx1A^Δ229/Syx1A[+], Yif1^doc-99 has partially lethal - majority die phenotype

(Kandasamy et al., 2021)

Syx1A^Δ229, smi26D¹/smi26D[+] has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

Syx1A^Δ229, smi26D¹ has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

Phenotype Manifest In

Enhancer of

Statement

Reference

Syx1A^Δ229/Syx1A[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

Syx1A^Δ229 is an enhancer of eye phenotype of αSnap^UAS.cPa, Scer\GAL4^GMR.PF

(Babcock et al., 2004)

Suppressor of

Statement

Reference

Syx1A^Δ229/Syx1A[+] is a suppressor of rhabdomere of eye photoreceptor cell | conditional | adult stage phenotype of Hsap\HTT^128Q.FL.UAS, Scer\GAL4^GMR.PF

(Romero et al., 2008)

Syx1A^Δ229/Syx1A[+] is a suppressor of ommatidium | adult stage phenotype of Hsap\HTT^128Q.FL.UAS, Scer\GAL4^GMR.PF

(Romero et al., 2008)

Syx1A^Δ229 is a suppressor of eye phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

Other

Statement

Reference

Syx1A^Δ229/Syx1A[+], Yif1^doc-1 has wing phenotype

(Kandasamy et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Syx1A^Δ229, Yif1^doc-1 double heterozygotes show a small, oblique wing phenotype.

(Kandasamy et al., 2021)

Expression of Snap^Scer\UAS.cPa from the P{UAS-Snap.P}1 in a Syx1A^Δ229 background results in an enhancement of the Snap^Scer\UAS.cPa phenotype in the eye, including increased necrosis around the perimeter of the compound eye and more dramatic furrowing at ommatidial junctions.

(Babcock et al., 2004)

Xenogenetic Interactions

Statement

Reference

Syx1A^Δ229/+ fully suppresses the increased excitatory junction potential (EJP) amplitude and decreased failure rate seen in Hsap\HD^{128Q.FL.Scer\UAS}, Scer\GAL4^elav-C155 animals at 0.25 mM Ca[2+].

Syx1A^Δ229/+ suppresses the reduced number of rhabdomeres per ommatidium phenotype seen in Hsap\HD^{128Q.FL.Scer\UAS}, Scer\GAL4^GMR.PF animals at 20 days.

Syx1A^Δ229/+ suppresses the external eye phenotype seen in Hsap\HD^{128Q.FL.Scer\UAS}, Scer\GAL4^GMR.PF animals.

Syx1A^Δ229/+ suppresses the impaired motor performance, as measured by a climbing assay, of Hsap\HD^{128Q.FL.Scer\UAS}, Scer\GAL4^C164 animals.

Syx1A^Δ229/+ reverses the increase in resting synaptic Ca[2+] levels at presynaptic terminals seen in Hsap\HD^{128Q.FL.Scer\UAS}, Scer\GAL4^elav-C155 larvae.

(Romero et al., 2008)

Complementation and Rescue Data

Complements

Syx1A^15ts

(Schulze and Bellen, 1996)

Syx1A¹⁷⁷

(Schulze and Bellen, 1996)

Syx1A⁴

(Schulze and Bellen, 1996)

Partially complements

Syx1A⁴

(Schulze and Bellen, 1996)

Rescued by

Syx1A^Δ229 is rescued by Syx1A^+t13.5

(Fergestad et al., 2001)

Syx1A^Δ229 is rescued by Syx1A^+t13.5

(Wu et al., 1999)

Syx1A^Δ229 is rescued by Syx1A^+t6

(Schulze and Bellen, 1996)

Syx1A^Δ229 is rescued by Syx1A^+t11

(Schulze and Bellen, 1996)

Partially rescued by

Syx1A^Δ229/Syx1A^3-69 is partially rescued by Scer\GAL4^elav-C155/Syx1A^UAS.cBa

(Lagow et al., 2007)

Syx1A^Δ229 is partially rescued by Syx1A^A243V.V247A

(Fergestad et al., 2001)

Syx1A^Δ229 is partially rescued by Syx1A^I212A

(Wu et al., 1999)

Not rescued by

Syx1A^Δ229 is not rescued by Syx1A^H3-C

(Wu et al., 1999)

Syx1A^Δ229 is not rescued by Syx1A^I236A

(Wu et al., 1999)

Syx1A^Δ229 is not rescued by Syx1A^H3-N

(Wu et al., 1999)

Comments

Lethality is rescued by one copy of the Syx1A^+t11 or Syx1A^+t6 constructs, rescued adults are viable, fertile and morphologically normal. 11%-22% Syx1A^Δ229/Syx1A¹⁷⁷ transheterozygous adults are viable.

(Schulze and Bellen, 1996)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4379

Syx1A^Δ229 ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Kyoto

107713

Syx1A^Δ229 ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

Shows normal GABA staining in stage 17 nerve cord.

(Kueppers et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

Delta229

(Kaltenbach et al., 2007)

Df(3R)Δ229

(Schulze and Bellen, 1996, Schulze et al., 1995)

Sx1a^Δ229

(Bademosi et al., 2017)

Syx1A^Δ229

(Barrecheguren et al., 2017, Pack-Chung et al., 2007)

Syx1A^δ229

(Tonning et al., 2006)

Syx²²⁹

(Romero et al., 2008)

Syx^Δ229

(Kandasamy et al., 2021)

syntaxin 1A^Δ229

(Simonsen et al., 2007)

syntaxin²²⁹

(Kueppers et al., 2003)

syx1A^Δ229

(Khuong et al., 2013, Babcock et al., 2004, Ruden et al., 2000, Stewart et al., 2000)

syx²²⁹

(Fergestad et al., 2001, Wu et al., 1999, Littleton et al., 1998, Broadie et al., 1995)

syxⁿ²²⁹

(Schulze and Bellen, 1996)

syx^Δ229

(Tian et al., 2013, Koles et al., 2012, Lagow et al., 2007, Featherstone et al., 2002, Stewart et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (26)

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