FlyBase Allele Report: Dmel\rn[roe.UAS]

General Information

Symbol

Dmel\rn^roe.UAS

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of St Pierre

FlyBase ID

FBal0137097

Feature type

allele

Associated gene

Dmel\rn

Associated Insertion(s)

Carried in Construct

P{UAS-roe.SP}

Key Links

Transgenic product class

genomic_DNA

(St Pierre et al., 2002)

wild_type

(St Pierre et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(St Pierre et al., 2002)

wild_type

(St Pierre et al., 2002)

Mutagen

in vitro construct

(St Pierre et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-roe.SP}

Cytology

Description

UASt regulatory sequences drive expression of the "roe" isoform of rn (nucleotides 0-2160 of AF395904 and 86bp of upstream genomic DNA).

(St Pierre et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PF

(St Pierre et al., 2002)

visible, with Scer\GAL4^rn-GAL4-5

(St Pierre et al., 2002)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(St Pierre et al., 2002)

interommatidial bristle, with Scer\GAL4^GMR.PF

(St Pierre et al., 2002)

pigment cell, with Scer\GAL4^GMR.PF

(St Pierre et al., 2002)

tarsal segment, with Scer\GAL4^rn-GAL4-5

(St Pierre et al., 2002)

Detailed Description

Statement

Reference

Expression of rn^roe.Scer\UAS under the control of Scer\GAL4^rn-GAL4-5 results in a reduced number of tarsal segments in the leg. Expression of rn^roe.Scer\UAS under the control of Scer\GAL4^GMR.PF disrupts the morphology and size of the adult eye, including loss of pigment and bristle cells. Patches of necrotic tissue are present. Expression of rn^roe.Scer\UAS under the control of Scer\GAL4^sev.EP results in little if any rough eye morphology.

(St Pierre et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

rn^roe.UAS/Scer\GAL4^sev.EP partially rescues Df(3R)rn20/rn¹⁶

(St Pierre et al., 2002)

Fails to rescue

rn^roe.UAS/Scer\GAL4^rn-GAL4-5 fails to rescue Df(3R)rn20/rn^GAL4-5

(St Pierre et al., 2002)

Comments

Expression of rn^roe.Scer\UAS under the control of Scer\GAL4^rn-GAL4-5 fails to rescue the leg defects of rn^GAL4-5/Df(3R)rn20 animals. Expression of rn^roe.Scer\UAS under the control of Scer\GAL4^sev.EP partially rescues the eye phenotypes of rn¹⁶/Df(3R)rn20 animals.