FlyBase Allele Report: Dmel\Kap-α1[UAS.cMa]

General Information

Symbol

Dmel\Kap-α1^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Mason

FlyBase ID

FBal0137475

Feature type

allele

Associated gene

Dmel\Kap-α1

Associated Insertion(s)

Carried in Construct

P{UAS-Kap-α1.M}

Key Links

Transgenic product class

wild_type

(Mason et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Mason et al., 2002)

Mutagen

in vitro construct

(Mason et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Kap-α1.M}

Cytology

Description

UASt regulatory sequences drive expression of Kap-α1.

(Mason et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Kap-α1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | heat sensitive, with Scer\GAL4^e22c

(Ratan et al., 2008)

Phenotype Manifest In

tergite, with Scer\GAL4^e22c

(Ratan et al., 2008)

Detailed Description

Statement

Reference

Expression of Kap-α1^Scer\UAS.cMa under the control of Scer\GAL4^e22c at 25[o]C results in partial lethality. Surviving adult escapers show a defect in abdominal development; in 96% of flies, the adult hemitergites fail to properly fuse at the dorsal midline.

Expression of Kap-α1^Scer\UAS.cMa under the control of Scer\GAL4^e22c at 27.5[o]C results in almost complete lethality.

(Ratan et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Kap-α1^UAS.cMa, Scer\GAL4^e22c has lethal phenotype, enhanceable by Cas[+]/Cse1^k03902

(Ratan et al., 2008)

Kap-α1^UAS.cMa, Scer\GAL4^e22c has lethal phenotype, enhanceable by Df(2L)H20/+

(Ratan et al., 2008)

NOT Enhanced by

Statement

Reference

Kap-α1^UAS.cMa, Scer\GAL4^e22c has lethal phenotype, non-enhanceable by Cse1^16-1/Cas[+]

(Ratan et al., 2008)

Suppressed by

Statement

Reference

Kap-α1^UAS.cMa, Scer\GAL4^e22c has lethal phenotype, suppressible by Cse1^UAS.cRa, Scer\GAL4^e22c

(Ratan et al., 2008)

Kap-α1^UAS.cMa, Scer\GAL4^e22c has lethal phenotype, suppressible | partially by Fs(2)Ket^RE34/Fs(2)Ket[+]

(Ratan et al., 2008)

Kap-α1^UAS.cMa, Scer\GAL4^e22c has lethal phenotype, suppressible | partially by Fs(2)Ket[+]/Fs(2)Ket^RX41

(Ratan et al., 2008)

Suppressor of

Statement

Reference

Kap-α1^UAS.cMa/Scer\GAL4^hs.PB is a suppressor of male sterile | recessive phenotype of Pen^D14

(Mason et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/Kap-α1^UAS.cMa is a non-suppressor of female sterile | recessive phenotype of Pen^D14

(Mason et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Kap-α1^UAS.cMa, Scer\GAL4^e22c has tergite phenotype, enhanceable by Cas[+]/Cse1^k03902

(Ratan et al., 2008)

Suppressed by

Statement

Reference

Kap-α1^UAS.cMa, Scer\GAL4^e22c has tergite phenotype, suppressible by Fs(2)Ket^RE34/Fs(2)Ket[+]

(Ratan et al., 2008)

Kap-α1^UAS.cMa, Scer\GAL4^e22c has tergite phenotype, suppressible by Fs(2)Ket[+]/Fs(2)Ket^RX41

(Ratan et al., 2008)

NOT suppressed by

Statement

Reference

Kap-α1^UAS.cMa, Scer\GAL4^e22c has phenotype, non-suppressible by Df(2L)Fs(2)Ket-RX22/+

(Ratan et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Surviving escapers expressing Kap-α1^Scer\UAS.cMa under the control of Scer\GAL4^e22c in a Cas^k03902/+ background have tergites that appear thinner than normal and retain only the posterior pigmented band, resulting in an apparent expansion of the intertergal region.

Heterozygosity for Fs(2)Ket^RE34 or Fs(2)Ket^RX41 results in a slight increase in the viability of animals expressing Kap-α1^Scer\UAS.cMa under the control of Scer\GAL4^e22c at 25[o]C and results in significantly increased viability of these animals at 27.5[o]C. The defects in tergite morphology are less severe in adult escapers expressing Kap-α1^Scer\UAS.cMa under the control of Scer\GAL4^e22c if they are also heterozygous for Fs(2)Ket^RE34 or Fs(2)Ket^RX41.

(Ratan et al., 2008)

Expression of Kap-α1^Scer\UAS.cMa under the control of Scer\GAL4^hs.PB using heat shock partially rescues the sterility of Pen^D14 males.

(Mason et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25399

w^*; P{UAS-Kap-α1.M}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Kap-α1^Scer\UAS.cMa

Kap-α1^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Mason

(Mason et al., 2002)

Secondary FlyBase IDs

References (3)

Report Sections

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