FB2024_03 , released June 25, 2024
Allele: Dmel\osk3'UTR.UASp
Open Close
General Information
Symbol
Dmel\osk3'UTR.UASp
Species
D. melanogaster
Name
FlyBase ID
FBal0143291
Feature type
allele
Associated gene
Dmel\osk
Associated Insertion(s)
Carried in Construct
P{UASp-osk.3'UTR}
Also Known As
UASp-osk3'UTR
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Filardo and Ephrussi, 2003)
Progenitor genotype
Carried in construct
P{UASp-osk.3'UTR}
Cytology
Description

UASp regulatory sequences are fused upstream of a StyI, NotI fragment from the osk genomic region, consisting of the entire osk 3'UTR and about 1kb of 3' flanking genomic sequence.

(Filardo and Ephrussi, 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
osk
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos produced by oskA87/Df(3R)p-XT103 females that express osk3'UTR.Scer\UAS.P\T, under the control of both Scer\GAL4otu.T:Hsim\VP16 and Scer\GAL4nos.UTR.T:Hsim\VP16, show the 'posterior group' phenotype.

      (Jenny et al., 2006)

      Only 1% of osk3'UTR.Scer\UAS.P\T; Scer\GAL4nos.UTR.T:Hsim\VP16 embryos show defects in the head cuticle.

      (Yano et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Scer\GAL4VP16.nanos.UTR, osk3'UTR.UASp has embryonic/first instar larval cuticle & embryonic head phenotype, enhanceable by Hrb27C[+]/Hrb27Ck02814

      (Yano et al., 2004)

      Scer\GAL4VP16.nanos.UTR, osk3'UTR.UASp has embryonic/first instar larval cuticle & embryonic head phenotype, enhanceable by Hrb27C[+]/Hrb27Ck16203

      (Yano et al., 2004)

      Scer\GAL4VP16.nanos.UTR, osk3'UTR.UASp has embryonic/first instar larval cuticle & embryonic head phenotype, enhanceable by bru1QB/aret[+]

      (Yano et al., 2004)

      Scer\GAL4VP16.nanos.UTR, osk3'UTR.UASp has embryonic/first instar larval cuticle & embryonic head phenotype, enhanceable by Hrb27C[+]/Hrb27C02647

      (Yano et al., 2004)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The penetrance and severity of embryonic cuticle patterning defects in osk3'UTR.Scer\UAS.P\T; Scer\GAL4nos.UTR.T:Hsim\VP16 embryos is enhanced by Hrb27C02647/+ (5% head defects and 1% bicaudal), Hrb27Ck16203/+ (4% head defects). Hrb27Ck02814/+ (8% head defects and 3% bicaudal) or aretQB/+ (4% head defects).

      (Yano et al., 2004)

      osk3'UTR.Scer\UAS.P\T can fully suppress the germ cell replication defects seen in Scer\GAL4nos.UTR.T:Hsim\VP16; aretScer\UAS.P\T.cFa ovarioles.

      (Filardo and Ephrussi, 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4VP16.otu, Scer\GAL4VP16.nanos.UTR, osk3'UTR.UASp partially rescues oskA87/Df(3R)p-XT103

      (Jenny et al., 2006)
      Comments

      Expression of osk3'UTR.Scer\UAS.P\T, under the control of both Scer\GAL4otu.T:Hsim\VP16 and Scer\GAL4nos.UTR.T:Hsim\VP16, rescues the early oogenesis and egg-less phenotype of oskA87/Df(3R)p-XT103 females and the karyosome defect. However, the 'posterior group' phenotype of the embryos produced from these females is not rescued.

      (Jenny et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      RNA transcribed from this transgene is sufficient to associate with stau protein.

      (Jenny et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      osk3'UTR.Scer\UAS.P\T
      osk3'UTR.UASp
      Name Synonyms
      Secondary FlyBase IDs
        References (6)