FlyBase Allele Report: Dmel\dome[UAS.EGFP]

General Information

Symbol

Dmel\dome^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0144360

Feature type

allele

Associated gene

Dmel\dome

Associated Insertion(s)

Carried in Construct

P{UAS-dome.EGFP}

Also Known As

UAS-dome-EGFP

Key Links

Transgenic product class

wild_type

(Ghiglione et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ghiglione et al., 2002)

Mutagen

in vitro construct

(Ghiglione et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-dome.EGFP}

Cytology

Description

UASt regulatory sequences drive expression of wild-type dome tagged at the C-terminal end with EGFP.

(Ghiglione et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dome

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with Scer\GAL4^slbo.2.6

(Ghiglione et al., 2002)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Buchon et al., 2010)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Ghiglione et al., 2002)

midgut, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

Detailed Description

Statement

Reference

Ectopic expression of dome^{Scer\UAS.T:Avic\GFP-EGFP} in intestinal stem cells (ISCs) under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B induces ISC proliferation.

(Buchon et al., 2010)

Expression of dome^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^slbo.2.6 results in a failure of border follicle cell migration.

(Ghiglione et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, dome^UAS.EGFP has increased occurrence of cell division | adult stage phenotype, suppressible by Ras85D^NIG.9375R, Scer\GAL4^NP5130

(Buchon et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, dome^UAS.EGFP has midgut phenotype, suppressible by Ras85D^NIG.9375R, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B, dome^UAS.EGFP has midgut phenotype, suppressible by Egfr^DN.UAS.cBa, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Egfr^{DN.Scer\UAS.cBa} blocks the extra ISC proliferation induced by dome^{Scer\UAS.T:Avic\GFP-EGFP}-expression under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B.

Co-expression of Ras85D^NIG.9375R blocks the extra ISC proliferation induced by dome^{Scer\UAS.T:Avic\GFP-EGFP}-expression under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B.

(Buchon et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dome^{Scer\UAS.T:Avic\GFP-EGFP}

dome^UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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