FlyBase Allele Report: Dmel\Krn[s.UAS.cUa]

General Information

Symbol

Dmel\Krn^s.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0144767

Feature type

allele

Associated gene

Dmel\Krn

Associated Insertion(s)

Carried in Construct

P{UAS-Krn.s.U}

Key Links

Transgenic product class

inframe_deletion

(Urban et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Urban et al., 2002)

Mutagen

in vitro construct

(Urban et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Krn.s.U}

Cytology

Description

Expression of a predicted secreted form of Krn (truncated at residue 112) is under the control of UASt sequences.

(Urban et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Krn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Buchon et al., 2010)

lethal, with Scer\GAL4^cb41

(McDonald et al., 2006)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(McDonald et al., 2006)

border follicle cell | somatic clone, with Scer\GAL4^cb41

(McDonald et al., 2006)

denticle | ectopic, with Scer\GAL4^arm.PS

(Urban et al., 2002)

midgut, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

midgut, with Scer\GAL4^unspecified

(Buchon et al., 2010)

wing, with Scer\GAL4^Bx-MS1096

(Urban et al., 2002)

wing vein | ectopic, with Scer\GAL4^Bx-MS1096

(Urban et al., 2002)

Detailed Description

Statement

Reference

Expression of Krn^{s.Scer\UAS.cUa} in precursor cells under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B induces high level intestinal stem cell (ISC) proliferation.

Overexpression of Krn^{s.Scer\UAS.cUa} driven by Scer\GAL4^unspecified results in a thick multi-layering of the gut due to the accumulation of cells in the lumen.

(Buchon et al., 2010)

Border cells are found away from their normal migration pathway on the side of the egg chamber when Krn^{s.Scer\UAS.cUa} is expressed under the control of Scer\GAL4^cb41 in follicle cell clones. Due to the lethality caused by expression of Krn^{s.Scer\UAS.cUa} under the control of Scer\GAL4^cb41, this phenotype was observed in adult flies for whom expression of Krn^{s.Scer\UAS.cUa} was initially inhibited by Scer\GAL80^ts.αTub84B and this inhibition was relieved by a temperature shift after the lethal stage. Expression of two copies of the Krn^{s.Scer\UAS.cUa} transgene results in a higher percentage of egg chambers with misguided border cells than expression of one copy. The penetrance of the phenotype also varies depending on the Krn^{s.Scer\UAS.cUa} insertion (P{UAS-Krn.s.U}2 causes a higher penetrance than P{UAS-Krn.s.U}3).

Expression of one copy of Krn^{s.Scer\UAS.cUa} in the border cell cluster, driven by Scer\GAL4^slbo.2.6, results in the inhibition of border cell migration in over 90% of egg chambers.

(McDonald et al., 2006)

Krn^{s.Scer\UAS.cUa}; Scer\GAL4^Bx-MS1096 flies have blistered wings with thickened and ectopic veins. Extra denticles are produced in Krn^{s.Scer\UAS.cUa}; Scer\GAL4^arm.PS embryos.

(Urban et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Krn^s.UAS.cUa/Scer\GAL4^vn-GAL4 is a suppressor | partially of lethal - all die before end of pupal stage phenotype of vn^L6/vn^GAL4

(Austin et al., 2014)

Other

Statement

Reference

Krn^s.UAS.cUa, Scer\GAL4^vn-GAL4, vn^L6/vn^GAL4 has lethal - all die during embryonic stage phenotype

(Austin et al., 2014)

Phenotype Manifest In

Other

Statement

Reference

Krn^s.UAS.cUa, Scer\GAL4^vn-GAL4, vn^L6/vn^GAL4 has wing vein | ectopic phenotype

(Austin et al., 2014)

Krn^s.UAS.cUa, Scer\GAL4^vn-GAL4, vn^L6/vn^GAL4 has wing vein phenotype

(Austin et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Krn^{s.Scer\UAS.cUa} under the control of Scer\GAL4^vn.GAL4 rescues the lethality of vn^L6/vn^GAL4 mutants to the pharate adult stage. The wings have fused L3 and L4 veins and extra vein material. The wing disc phenotype is partially rescues and no leg defects are observed. The extent of the rescue depends on the level of expression, but viable flies are never seen. Very high levels of expression are toxic, with animals dying as embryos.

(Austin et al., 2014)

Coexpression of spi^s.Scer\UAS and Krn^{s.Scer\UAS.cUa}, under the control of Scer\GAL4^cb41, in follicle cell clones results in a higher penetrance of the border cell misguidance phenotype compared to expression of either transgene alone, although the effect is additive rather than synergistic.

(McDonald et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Krn^{s.Scer\UAS.cUa}

Krn^s.UAS.cUa

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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