FlyBase Allele Report: Dmel\Thor[LL.UAS]

General Information

Symbol

Dmel\Thor^LL.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0147362

Feature type

allele

Associated gene

Dmel\Thor

Associated Insertion(s)

Carried in Construct

P{UAS-Thor.LL}

Also Known As

UAS-4E-BP LL

Key Links

Transgenic product class

missense_variant

(Miron et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Miron et al., 2001)

Mutagen

in vitro construct

(Miron et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Thor.LL}

Cytology

Description

UASt regulatory sequences drive expression of a mutated form of Thor (carries the amino acid replacements M59L and K60L).

(Miron et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Thor

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell size, with Scer\GAL4^Act5C.PP

(Roy et al., 2004)

abnormal feeding behavior, with Scer\GAL4^Mhc.PW

(Demontis and Perrimon, 2010)

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

abnormal neuroanatomy | larval stage, with Scer\GAL4^ppk.PU

(Niehues et al., 2015)

abnormal neuroanatomy | larval stage, with Scer\GAL4^VGlut-OK371

(Niehues et al., 2015)

abnormal stress response, with Scer\GAL4^arm.PS

(Zid et al., 2009)

decreased cell number, with Scer\GAL4^Bx-MS1096

(Miron et al., 2001)

decreased cell number | somatic clone, with Scer\GAL4^Act5C.PP

(Miron et al., 2001)

decreased cell size | somatic clone, with Scer\GAL4^Act5C.PP

(Miron et al., 2001)

decreased speed of aging, with Scer\GAL4^Mhc.PW

(Demontis and Perrimon, 2010)

long lived, with Scer\GAL4^Mhc.PW

(Demontis and Perrimon, 2010)

long lived | nutrition conditional, with Scer\GAL4^arm.PS

(Zid et al., 2009)

visible, with Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

visible, with Scer\GAL4^dpp.blk1

(Miron et al., 2001)

Phenotype Manifest In

1st posterior cell, with Scer\GAL4^dpp.blk1

(Miron et al., 2001)

adult abdominal fat mass, with Scer\GAL4^Mhc.PW

(Demontis and Perrimon, 2010)

adult brain, with Scer\GAL4^Mhc.PW

(Demontis and Perrimon, 2010)

embryonic/larval fat body, with Scer\GAL4^Act5C.PP

(Roy et al., 2004)

embryonic/larval neuromuscular junction, with Scer\GAL4^VGlut-OK371

(Niehues et al., 2015)

larval multidendritic class IV neuron | larval stage, with Scer\GAL4^ppk.PU

(Niehues et al., 2015)

muscle cell, with Scer\GAL4^Mhc.PW

(Demontis and Perrimon, 2010)

retina, with Scer\GAL4^Mhc.PW

(Demontis and Perrimon, 2010)

terminal bouton, with Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

wing, with Scer\GAL4^Bx-MS1096

(Roy et al., 2004, Miron et al., 2001)

wing disc, with Scer\GAL4^Bx-MS1096

(Roy et al., 2004, Miron et al., 2001)

wing disc | somatic clone, with Scer\GAL4^Act5C.PP

(Miron et al., 2001)

Detailed Description

Statement

Reference

Scer\GAL4^VGlut-OK371-mediated expression in motor neurons results in muscle 24 denervation in the vast majority of larvae.

Scer\GAL4^ppk.PU-mediated expression in class IV multidendritic sensory neurons induces severe morphology defects, with reduction of dendritic coverage by around 40%.

(Niehues et al., 2015)

Expression of Thor^LL.Scer\UAS under the control of Scer\GAL4^elav-C155 increases bouton size compared to controls.

(Deivasigamani et al., 2014)

Overexpression of Thor^LL.Scer\UAS in muscles under the control of Scer\GAL4^Mhc.PW significantly preserves muscle protein homeostasis, while the muscles of wild-type animals display increased accumulation of protein aggregates. Compared to syngenic controls, the age-related accumulation of protein aggregates in retinas, brains and the peripheral fat body of the abdomen is also decreased in flies overexpressing Thor^LL.Scer\UAS under the control of Scer\GAL4^Mhc.PW.

Muscle functionality gradually decreases in wild-type aging flies, resulting in impaired climbing and flight ability. Overexpression of Thor^LL.Scer\UAS under the control of Scer\GAL4^Mhc.PW significantly preserves muscle strength during aging.

Overexpression of Thor^LL.Scer\UAS under the control of Scer\GAL4^Mhc.PW in muscles is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.

Food intake of flies is decreased in response to overexpression of Thor^LL.Scer\UAS in muscles via Scer\GAL4^Mhc.PW.

The body weight of adult flies overexpressing Thor^LL.Scer\UAS under the control of Scer\GAL4^Mhc.PW does not significantly differ from that of syngenic controls.

There is a significant decrease in the hemolymph glucose concentration compared with syngenic control in flies overexpressing Thor^LL.Scer\UAS under the control of Scer\GAL4^Mhc.PW.

(Demontis and Perrimon, 2010)

Overexpression of Thor^LL.Scer\UAS under the control of Scer\GAL4^arm.PS extends the mean life-span of females by 14% on rich food, but does not seem to affect males. In contrast, under dietary restriction, there is no lifespan extension, beyond the effect of dietary restriction alone.

(Zid et al., 2009)

When expression is driven in the wing, by Scer\GAL4^Bx-MS1096, wing size is reduced, though pattern is unaffected. The effect on size is evident in the wing disc. The reduction in size is caused by reduction in cell number. When expression is driven in the eye disc, via Scer\GAL4^GMR.PF, the resulting eyes, though normally patterned, have ommatidia reduced in size (not number) compared to those of control eyes. When expression is driven by Scer\GAL4^Act5C.PP, in clones in the larval fat body, the size of the fat body cells is reduced.

(Roy et al., 2004)

Expression of Thor^LL.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 using the P{UAS-Thor.LL}w transgene causes a marked reduction in wing size (11.2 +/- 3.4%) without affecting cell number. Wing discs in these animals are indistinguishable from control discs. Expression of Thor^LL.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 using the P{UAS-Thor.LL}s transgene causes a marked reduction in wing size (37.1 +/- 2.7%) which is partly due to a decrease in cell number (19.0 +/- 4.3%). Wing discs in these animals are 52% smaller than normal and contain many apoptotic cells. The flies take 1-2 days longer to eclose than normal. Clones in the wing disc expressing Thor^LL.Scer\UAS under the control of Scer\GAL4^Act5C.PP using the P{UAS-Thor.LL}w transgene have fewer cells than wild-type clones. Few clones in the wing disc expressing Thor^LL.Scer\UAS under the control of Scer\GAL4^Act5C.PP using the P{UAS-Thor.LL}s transgene are recovered and they usually contain 1-2 cells. Clones in the wing disc expressing Thor^LL.Scer\UAS under the control of Scer\GAL4^Act5C.PP are smaller in size than wild type. Clones in the fat body expressing Thor^LL.Scer\UAS under the control of Scer\GAL4^Act5C.PP using the P{UAS-Thor.LL}s transgene are 45-70% smaller than neighbouring wild-type cells. Clones in the fat body expressing Thor^LL.Scer\UAS under the control of Scer\GAL4^Act5C.PP using the P{UAS-Thor.LL}s transgene are recovered in the posterior of the eye disc but are absent from the anterior of the eye disc and the antennal disc. Expression of Thor^LL.Scer\UAS under the control of Scer\GAL4^dpp.blk1 using the P{UAS-Thor.LL}s transgene results in reduction in size of the region encompassed by wing veins 3 and 4, the anterior crossvein and the wing margin.

(Miron et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^Mhc.PW, Thor^LL.UAS has long lived phenotype, non-suppressible by foxo²¹/foxo[+]

(Demontis and Perrimon, 2010)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Thor^LL.UAS is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, Vap33^UAS.cRa

(Deivasigamani et al., 2014)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Thor^LL.UAS is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

Other

Statement

Reference

Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF, Thor^LL.UAS has lethal phenotype

(Feuillette et al., 2020)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, Thor^LL.UAS has wing disc phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^Bx-MS1096

(Miron et al., 2001)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Thor^LL.UAS is a suppressor of terminal bouton phenotype of Scer\GAL4^elav-C155, Vap33^UAS.cRa

(Deivasigamani et al., 2014)

NOT Suppressor of

Statement

Reference

Thor^LL.UAS, Scer\GAL4^fln.IFM is a non-suppressor of indirect flight muscle cell | adult stage phenotype of LamC^R205W.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

Thor^LL.UAS, Scer\GAL4^fln.IFM is a non-suppressor of indirect flight muscle cell | adult stage phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

Scer\GAL4^elav-C155/Thor^LL.UAS is a non-suppressor of terminal bouton phenotype of Scer\GAL4^elav-C155, Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of LamC^UAS.cSa and Thor^LL.UAS under the control of Scer\GAL4^fln.IFM under the control of Scer\GAL4^fln.IFM does not lead to any obvious indirect flight muscle, wing posture or flight defects.

(Chandran et al., 2019)

Expression of Thor^LL.Scer\UAS does not suppress the enlarged bouton phenotype seen when Vap-33A^{P58S.cRa.Scer\UAS} is expressed under the control of Scer\GAL4^elav-C155.

Expression of Thor^LL.Scer\UAS suppresses the small bouton phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^elav-C155.

(Deivasigamani et al., 2014)

Overexpression of Thor^LL.Scer\UAS under the control of Scer\GAL4^Mhc.PW in a heterozygous foxo²¹ mutant genetic background is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.

(Demontis and Perrimon, 2010)

Co-expression of BacA\p35^Scer\UAS.cHa partially rescues the reduced size of wing discs in animals expressing Thor^LL.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 using the P{UAS-Thor.LL}s transgene. The number of cells in clones in the wing disc expressing Thor^LL.Scer\UAS under the control of Scer\GAL4^Act5C.PP using the P{UAS-Thor.LL}w is not affected by co-expression of BacA\p35^Scer\UAS.cHa. Co-expression of BacA\p35^Scer\UAS.cHa greatly increases the number of clones recovered in the wing disc expressing Thor^LL.Scer\UAS under the control of Scer\GAL4^Act5C.PP using the P{UAS-Thor.LL}s transgene, but only marginally increases the number of cells per clone. The region of the wing encompassed by wing veins 3 and 4, the anterior crossvein and the wing margin is reduced in size when Thor^LL.Scer\UAS is expressed under the control of Scer\GAL4^dpp.blk1 using the P{UAS-Thor.LL}s transgene in a Df(2L)Pi3K21B-A/+; P{U2af38.gR10} background.

(Miron et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

8651

w¹¹¹⁸; P{UAS-p35.H}BH1; P{UAS-Thor.LL}s

24854

w^*; P{UAS-Thor.LL}s/TM6C, cu¹ Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Thor^LL.Scer\UAS

Thor^LL.UAS

thor^LL

(Lee et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (16)

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