FB2024_03 , released June 25, 2024
Allele: Dmel\srlKG08646
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General Information
Symbol
Dmel\srlKG08646
Species
D. melanogaster
Name
FlyBase ID
FBal0148128
Feature type
allele
Associated gene
Dmel\srl
Associated Insertion(s)
P{SUPor-P}srlKG08646
Carried in Construct
Also Known As
Srl1
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

hypomorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Tiefenböck et al., 2010)

hypomorphic allele - molecular evidence

(Tiefenböck et al., 2010)
Mutagen
P-element activity
(Gene Disruption Project members, 2001-)
Progenitor genotype
Associated Insertion(s)
P{SUPor-P}srlKG08646
Cytology
Description
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Parkinson's disease
      CEA
      (Ng et al., 2017)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      srlKG08646/srlKG08646 flies exhibit an age-dependent defect in climbing ability, loss of dopaminergic neurons from the PPL1 and PPM3 clusters; mitochondria in dopaminergic neurons from these flies exhibit a significant decrease in size, and mitochondria in the flight muscle are visibly more fragmented, as compared to controls.

      (Ng et al., 2017)

      srlKG08646/srlKG08646 flies have a significantly reduced lifespan compared to controls. Fecundity in mutant females (but not males) is severely compromised; ovaries develop more slowly and carry about half the number of ovarioles compared to wild type.

      (Mukherjee et al., 2014)

      Homozygous srlKG08646 mutant adults are viable. srlKG08646 females are sterile. Both mutant males and females have an approximately 25% reduction in wet weight compared with controls. When normalised to body weight, reduced lipid and glycogen levels are observed in adult males demonstrating metabolic defects. Size defects are not observed when external structures derived from imaginal discs, such as wings or legs, are analysed.

      A trans-heterozygous combination of srlKG08646 with Df(3R)ED5046 does not lead to a further decrease in adult weight compared with srlKG08646 homozygous animals.

      Whereas control larvae enter the third instar 72 h after egg deposition (AED) and pupate 120 h AED, srlKG08646 mutants enter with a 12-14-h delay, and pupate with a 24-h delay.

      The larval fat body of homozygous srlKG08646 mutants show normal abundance of mitochondria. The morphology of mitochondria appear normal in srlKG08646 mutants.

      Enlarged lipid droplets are observed in srlKG08646 mutant fat body cells.

      Mitochondrial DNA content (normalised to nuclear DNA) in srlKG08646 mutants is similar to that of controls.

      (Tiefenböck et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      srlKG08646 has abnormal developmental rate phenotype, enhanceable by Ets97D613

      (Tiefenböck et al., 2010)
      NOT Enhancer of
      Statement
      Reference

      srlKG08646 is a non-enhancer of decreased body size phenotype of chicoflp147E/chico1

      (Tiefenböck et al., 2010)
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      srlKG08646 is an enhancer of fat body phenotype of Ets97D613

      (Tiefenböck et al., 2010)

      srlKG08646 is an enhancer of mitochondrion phenotype of Ets97D613

      (Tiefenböck et al., 2010)

      srlKG08646 is an enhancer of mitochondrial chromosome phenotype of Ets97D613

      (Tiefenböck et al., 2010)
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      While both single mutants are viable to adulthood, foxounspecified, srlKG08646 double mutants show larval lethality.

      There is no further weight reduction in chicoflp147E/chico1, srlKG08646 double mutants.

      srlKG08646, Ets97D613 double mutants show a more severe mitochondrial phenotype in the fat body. The abundance of mitochondria in the fat body is further reduced compared with Ets97D613 single mutants. In contrast to the phenotype in the single mutants, the mitochondria in double mutants are rounded in shape and the inner-mitochondrial cristae are either missing or strongly reduced in size.

      Mitochondrial DNA content (normalised to nuclear DNA) in srlKG08646, Ets97D613 double mutants is further increased compared to that of Ets97D613 single mutants.

      The delay in pupation is increased to four days in srlKG08646, Ets97D613 double mutants.

      The effects of Scer\GAL4αTub84B.PP>InRScer\UAS.cHa overexpression on cell growth and lipid droplet size in fat body cell clones are completely abrogated in a srlKG08646 mutant genetic background.

      The effect of Scer\GAL4αTub84B.PP>Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX overexpression on cell growth in fat body cell clones is suppressed by srlKG08646.

      (Tiefenböck et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      CG9809KG08646
      SpargelKG08646
      Srl1
      (Tiefenböck et al., 2010)
      srl1
      (Damschroder et al., 2022, Basar et al., 2019, Ng et al., 2017, Mukherjee et al., 2014, Tiefenböck et al., 2010)
      srlKG08646
      (Ng et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)