FlyBase Allele Report: Dmel\Cap-D3[EY00456]

General Information

Symbol

Dmel\Cap-D3^EY00456

Species

D. melanogaster

Name

FlyBase ID

FBal0148241

Feature type

allele

Associated gene

Dmel\Cap-D3

Associated Insertion(s)

P{EPgy2}Cap-D3^EY00456

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}Cap-D3^EY00456

Cytology

Description

Insertion in the third exon.

(Hartl et al., 2008)

Insertion in the Cap-D3 open reading frame.

(Jiang et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:5,532,800..5,532,800 [+]

(Hartl et al., 2008, Jiang et al., 2007, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Hartl et al., 2008)

female fertile

(Jiang et al., 2007)

female fertile (with Cap-D3^GS12862)

(Jiang et al., 2007)

male fertile

(Jiang et al., 2007)

male fertile (with Cap-D3^GS12862)

(Jiang et al., 2007)

male sterile

(Hartl et al., 2008)

male sterile (with Df(2L)Exel6023)

(Hartl et al., 2008)

viable

(Jiang et al., 2007)

viable (with Cap-D3^GS12862)

(Jiang et al., 2007)

Phenotype Manifest In

polytene chromosome & nurse cell (with Df(2L)Exel7023)

(Hartl et al., 2008)

primary spermatocyte in meiotic anaphase I

(Hartl et al., 2008)

primary spermatocyte in meiotic prophase I

(Hartl et al., 2008)

Detailed Description

Statement

Reference

Homozygous Cap-D3^EY00456 and Cap-D3^EY00456/Df(2L)Exel6023 mutant males are completely sterile when mated to wild-type females.

In male Cap-D3^EY00456 mutants, chromosomal organizational steps throughout prophase I are defective, as normal territory formation is never observed in 100% of S2, S4, and S6 stages. Instead, chromatin is seemingly dispersed within the nucleus.

In two cysts observed from Cap-D3^EY00456 homozygous males, 7 of 20 anaphase I figures are bridged. This anaphase I bridging most likely represents a failure to resolve chromosomal associations prior to segregation as chromatin appears to be stretched between chromosomes moving to opposing poles.

(Hartl et al., 2008)

Polyteny persists in 91.3% of the nurse cells in stage 10 egg chambers of Cap-D3^EY00456/Df(2L)Exel7023 females (in contrast to wild-type nurse cells, which disassemble their polytene chromosomes during mid-oogenesis).

(Hartl et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Cap-D3^EY00456/Df(2L)Exel7023 has polytene chromosome & nurse cell phenotype, enhanceable by Cap-H2^Z3-0019/Cap-H2[+]

(Hartl et al., 2008)

Suppressor of

Statement

Reference

Cap-D3^EY00456/Df(2L)Exel7023 is a suppressor of polytene chromosome & larval salivary gland phenotype of Cap-H2^EY09979, Scer\GAL4^hs.PB

(Hartl et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The persistence of polyteny that is seen in the nurse cells of stage 10 egg chambers of Cap-D3^EY00456/Df(2L)Exel7023 females is enhanced by Cap-H2^Z3-0019/+; the penetrance of the phenotype is enhanced from 91.3 +/- 2.3% to 100%.

The polytene chromosome disassembly that is seen in larval salivary glands expressing Cap-H2^EY09979 under the control of Scer\GAL4^hs.PB is suppressed by Cap-D3^EY00456/Df(2L)Exel7023.

(Hartl et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

15026

y¹ w^67c23; P{EPgy2}Cap-D3^EY00456

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG31989^EY00456

Cap-D3^EY00456

(Vernizzi and Lehner, 2021, Hartl et al., 2008, Hartl et al., 2008, Hartl et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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