FB2024_03 , released June 25, 2024
Allele: Dmel\BuffyΔN.UAS.Tag:FLAG
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General Information
Symbol
Dmel\BuffyΔN.UAS.Tag:FLAG
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Quinn
FlyBase ID
FBal0150945
Feature type
allele
Associated gene
Dmel\Buffy
Associated Insertion(s)
Carried in Construct
P{UAS-Buffy.ΔN.FLAG}
Key Links
Transgenic product class
inframe_deletion
(Quinn et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Quinn et al., 2003)
Mutagen
in vitro construct
(Quinn et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-Buffy.ΔN.FLAG}
Cytology
Description

UASt sequences drives expression of an N terminal deleted Buffy tagged with Tag:FLAG. The first 128 amino acids have been removed (deleting the α1 and α2 domains).

(Quinn et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Buffy
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When these embryos are γ irradiated the amount of cell death seen is much reduced compared to wild-type.

      (Quinn et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhancer of
      Statement
      Reference

      BuffyΔN.UAS.Tag:FLAG, Scer\GAL4GMR.PF is a non-enhancer of eye phenotype of DroncUAS.EGFP, Scer\GAL4GMR.PF

      (Quinn et al., 2003)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      BuffyΔN.UAS.Tag:FLAG, Scer\GAL4GMR.PF is a non-suppressor of eye phenotype of DroncUAS.EGFP, Scer\GAL4GMR.PF

      (Quinn et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      BuffyΔN.Scer\UAS.T:Zzzz\FLAG
      BuffyΔN.UAS.Tag:FLAG
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Quinn
      (Quinn et al., 2003)
      Secondary FlyBase IDs
        References (1)