FlyBase Allele Report: Dmel\Dronc[UAS.EGFP]

General Information

Symbol

Dmel\Dronc^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0122046

Feature type

allele

Associated gene

Dmel\Dronc

Associated Insertion(s)

Carried in Construct

P{UAS-Dronc.EGFP}

Also Known As

UAS-dronc, UAS-dronc-GFP

Key Links

Transgenic product class

wild_type

(Quinn et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Quinn et al., 2000)

Mutagen

in vitro construct

(Quinn et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Dronc.EGFP}

Cytology

Description

UASt regulatory sequences drive expression of wild-type Dronc tagged with EGFP.

(Quinn et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Dronc

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.2N4R.cSa

(Asadzadeh et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death, with Scer\GAL4^Crz.504

(Lee et al., 2011)

increased cell death, with Scer\GAL4^GMR.PF

(Quinn et al., 2000)

increased cell death, with Scer\GAL4^hs.PB

(Quinn et al., 2000)

lethal | larval stage, with Scer\GAL4^Adh.PF

(Liu et al., 2009)

lethal | P-stage, with Scer\GAL4^Adh.PF

(Liu et al., 2009)

lethal - all die before end of pupal stage, with Scer\GAL4^GMR.PF

(Quinn et al., 2000)

short lived, with Scer\GAL4^elav-C155

(Bauer et al., 2005)

some die during pupal stage, with Scer\GAL4^GMR.PF

(Quinn et al., 2000)

visible, with Scer\GAL4^GMR.PF

(Quinn et al., 2000)

visible | adult stage, with Scer\GAL4^GMR.PU

(Park et al., 2023)

Phenotype Manifest In

embryonic/larval fat body | wandering third instar larval stage, with Scer\GAL4^Adh.PF

(Liu et al., 2009)

eye, with Scer\GAL4^GMR.PF

(Quinn et al., 2003, Quinn et al., 2000)

eye, with Scer\GAL4^GMR.PU

(Park et al., 2023)

eye disc | posterior, with Scer\GAL4^GMR.PF

(Quinn et al., 2000)

neuron, with Scer\GAL4^Crz.504

(Lee et al., 2011)

ommatidium, with Scer\GAL4^GMR.PF

(Quinn et al., 2000)

pigment cell, with Scer\GAL4^GMR.PF

(Quinn et al., 2000)

Detailed Description

Statement

Reference

Expression of Nc^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Crz.504 results in apoptosis in all vCrz neurons, but not in proto-cerebral corazonergic neurons.

(Lee et al., 2011)

Approximately 95% of larvae expressing Scer\GAL4^Adh.PF-driven Nc^{Scer\UAS.T:Avic\GFP-EGFP} in the fat body die during different larval stages. The remaining animals die at the pupal stage. A significant portion of fat body cells in these mutants display apoptosis at the early wandering third instar larval stage.

(Liu et al., 2009)

Expression of Nc^{Scer\UAS.T:Avic\GFP-EGFP}, under the control of Scer\GAL4^elav-C155, shortens lifespan in flies.

(Bauer et al., 2005)

Nc^{Scer\UAS.T:Avic\GFP-EGFP}; Scer\GAL4^hs.PB rescues pupariation of most Nc³⁰, Nc¹¹ or Nc⁵¹ homozygous larvae. It also rescues cell death in the larval midgut in response to feeding mid-third instar larvae with ecdysone and the normal pattern of cell death of abdominal neuroblasts in the late third instar.

(Waldhuber et al., 2005)

When Nc^{Scer\UAS.T:Avic\GFP-EGFP} is driven by Scer\GAL4^GMR.PF, animals have small mottled eyes.

(Quinn et al., 2003)

Expression of Nc^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF results in massive induction of cell death in the posterior part of the eye imaginal disc. Expression of Nc^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^hs.PB during embryogenesis or larval development using heat shock results in ectopic cell death. Many animals expressing Nc^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF die as pupae. The few adults that survive have severely ablated eyes. Insertions of P{UAS-Nc.EGFP} that result in less lethality when expressed under the control of Scer\GAL4^GMR.PF give rise to adult flies with slightly rough and mottled eyes at 25^oC. The phenotype is more severe at 29^oC.

(Quinn et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Dronc^UAS.EGFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Erasp^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Park et al., 2023)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has lethal phenotype, enhanceable by Df(3L)brm11

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Df(3L)brm11

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has lethal phenotype, enhanceable by Diap1⁵

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Diap1⁵

(Quinn et al., 2000)

NOT Enhanced by

Statement

Reference

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has visible phenotype, non-enhanceable by Df(2R)Jp1

(Quinn et al., 2000)

Suppressed by

Statement

Reference

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Df(3L)H99/+

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Diap2^GMR.PH

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Diap1^GMR.PH

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has visible phenotype, suppressible by BacA\p35^GMR.PH

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Dark^CD4

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Dark^CD8

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Dark^k11502

(Quinn et al., 2000)

NOT suppressed by

Statement

Reference

Dronc^UAS.EGFP, Scer\GAL4^Crz.504 has abnormal cell death phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Crz.504

(Lee et al., 2011)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has visible phenotype, non-suppressible by Df(2R)Jp1

(Quinn et al., 2000)

Other

Statement

Reference

Dronc^UAS.EGFP, Erasp^UAS.Tag:HA, Scer\GAL4^GMR.PU has abnormal eye color phenotype

(Park et al., 2023)

BacA\p35^UAS.cHa, Dronc^UAS.EGFP, Scer\GAL4^en-e16E has increased cell number | larval stage phenotype

(Wells et al., 2006)

BacA\p35^UAS.cHa, Dronc^UAS.EGFP, Scer\GAL4^en-e16E has increased occurrence of cell division | larval stage phenotype

(Huh et al., 2004)

BacA\p35^UAS.cHa, Dronc^UAS.EGFP, Scer\GAL4^ptc-559.1 has increased occurrence of cell division | larval stage phenotype

(Huh et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Dronc^UAS.EGFP, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Erasp^UAS.Tag:HA, Scer\GAL4^GMR.PU

(Park et al., 2023)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Df(3L)brm11

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Diap1⁵

(Quinn et al., 2000)

NOT Enhanced by

Statement

Reference

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Buffy^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF

(Quinn et al., 2003)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Buffy^UAS.cQa, Scer\GAL4^GMR.PF

(Quinn et al., 2003)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Df(2R)Jp1

(Quinn et al., 2000)

Suppressed by

Statement

Reference

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Dark^CD4

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Dark^CD8

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Dark^k11502

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Df(3L)H99/+

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Diap2^GMR.PH

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Diap1^GMR.PH

(Quinn et al., 2000)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, suppressible by BacA\p35^GMR.PH

(Quinn et al., 2000)

NOT suppressed by

Statement

Reference

Dronc^UAS.EGFP, Scer\GAL4^Crz.504 has neuron phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Crz.504

(Lee et al., 2011)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Buffy^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PF

(Quinn et al., 2003)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Buffy^UAS.cQa, Scer\GAL4^GMR.PF

(Quinn et al., 2003)

Dronc^UAS.EGFP, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Df(2R)Jp1

(Quinn et al., 2000)

Other

Statement

Reference

BacA\p35^UAS.cHa, Dronc^UAS.EGFP, Scer\GAL4^en-e16E has wing disc phenotype

(Wells et al., 2006)

BacA\p35^UAS.cHa, Dronc^UAS.EGFP, Scer\GAL4^en-e16E has wing disc posterior compartment | larval stage phenotype

(Huh et al., 2004)

BacA\p35^UAS.cHa, Dronc^UAS.EGFP, Scer\GAL4^ptc-559.1 has wing disc | larval stage phenotype

(Huh et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Wing discs coexpressing BacA\p35^Scer\UAS.cHa and Nc^{Scer\UAS.T:Avic\GFP-EGFP} under the control of either Scer\GAL4^en-e16E or Scer\GAL4^ptc-559.1 show increased cell proliferation in the domain of Scer\GAL4 expression.

(Huh et al., 2004)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa with Nc^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Crz.504 does not prevent the Nc-induced apoptosis in vCrz neurons.

(Lee et al., 2011)

Expression of Nc^{Scer\UAS.T:Avic\GFP-EGFP} and BacA\p35^Scer\UAS.cHa in the wing disc, driven by Scer\GAL4^en-e16E, induces a strong overproliferation phenotype.

(Wells et al., 2006)

Complementation and Rescue Data

Partially rescues

Dronc^UAS.EGFP/Scer\GAL4^hs.PB partially rescues Dronc⁵¹

(Waldhuber et al., 2005)

Dronc^UAS.EGFP/Scer\GAL4^hs.PB partially rescues Dronc¹¹

(Waldhuber et al., 2005)

Dronc^UAS.EGFP/Scer\GAL4^hs.PB partially rescues Dronc³⁰

(Waldhuber et al., 2005)

Comments

Nc^{Scer\UAS.T:Avic\GFP-EGFP}; Scer\GAL4^hs.PB rescues pupariation of most Nc³⁰, Nc¹¹ or Nc⁵¹ homozygous larvae. It also rescues cell death in the larval midgut in response to feeding mid-thrd instar larvae with ecdysone and the normal pattern of cell death of abdominal neuroblasts in the late third instar.

(Waldhuber et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

56758

w^*; P{UAS-Dronc.EGFP}3

56759

w^*; P{UAS-Dronc.EGFP}2

Notes on Origin

Discoverer