FlyBase Allele Report: Dmel\LIMK1[D522A.UAS]

General Information

Symbol

Dmel\LIMK1^D522A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0156298

Feature type

allele

Associated gene

Dmel\LIMK1

Associated Insertion(s)

Carried in Construct

P{UAS-LIMK1.D522A}

Key Links

Transgenic product class

dominant_negative_variant

(Chen et al., 2004)

missense_variant

(Chen et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Chen et al., 2004)

missense_variant

(Chen et al., 2004)

Mutagen

in vitro construct

(Chen et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-LIMK1.D522A}

Cytology

Description

UAS regulatory sequences drive expression of a kinase-deficient form of LIMK1. Encodes a dominant negative protein.

(Chen et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

LIMK1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile, with Scer\GAL4^T80

(Chen et al., 2004)

viable, with Scer\GAL4^T80

(Chen et al., 2004)

visible, with Scer\GAL4^hs.PB

(Chen et al., 2004)

visible, with Scer\GAL4^T80

(Chen et al., 2004)

Phenotype Manifest In

femur, with Scer\GAL4^T80

(Chen et al., 2004)

leg, with Scer\GAL4^hs.PB

(Chen et al., 2004)

leg, with Scer\GAL4^T80

(Chen et al., 2004)

tibia, with Scer\GAL4^T80

(Chen et al., 2004)

wing, with Scer\GAL4^T80

(Chen et al., 2004)

Detailed Description

Statement

Reference

Flies expressing LIMK1^{D522A.Scer\UAS} under the control of Scer\GAL4^T80 have small, malformed wings. Their legs are malformed, with the femur being bent and twisted, while the tibia is often shorter than normal and twisted. Flies co-expressing LIMK1^Scer\UAS.cCa and LIMK1^{D522A.Scer\UAS} under the control of Scer\GAL4^en-e16E have normal wings. Approximately 5% of flies expressing LIMK1^{D522A.Scer\UAS} under the control of Scer\GAL4^hs.PB have a malformed leg phenotype.

(Chen et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

LIMK1^D522A.UAS, Scer\GAL4^hs.PB has visible phenotype, enhanceable by br¹

(Chen et al., 2004)

LIMK1^D522A.UAS, Scer\GAL4^hs.PB has visible phenotype, enhanceable by Sb[+]/Sb⁷⁰

(Chen et al., 2004)

LIMK1^D522A.UAS, Scer\GAL4^hs.PB has visible phenotype, enhanceable by Sb^63b/Sb[+]

(Chen et al., 2004)

Enhancer of

Statement

Reference

LIMK1^D522A.UAS/Scer\GAL4^hs.PB is an enhancer of visible | dominant phenotype of Sb⁷⁰

(Chen et al., 2004)

LIMK1^D522A.UAS/Scer\GAL4^hs.PB is an enhancer of visible | dominant phenotype of Sb^63b

(Chen et al., 2004)

LIMK1^D522A.UAS/Scer\GAL4^hs.PB is an enhancer of visible | dominant phenotype of br¹

(Chen et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

LIMK1^D522A.UAS, Scer\GAL4^hs.PB has leg phenotype, enhanceable by br¹

(Chen et al., 2004)

LIMK1^D522A.UAS, Scer\GAL4^hs.PB has leg phenotype, enhanceable by Sb[+]/Sb⁷⁰

(Chen et al., 2004)

LIMK1^D522A.UAS, Scer\GAL4^hs.PB has leg phenotype, enhanceable by Sb^63b/Sb[+]

(Chen et al., 2004)

Enhancer of

Statement

Reference

LIMK1^D522A.UAS/Scer\GAL4^hs.PB is an enhancer of leg phenotype of Sb⁷⁰

(Chen et al., 2004)

LIMK1^D522A.UAS/Scer\GAL4^hs.PB is an enhancer of leg phenotype of Sb^63b

(Chen et al., 2004)

LIMK1^D522A.UAS/Scer\GAL4^hs.PB is an enhancer of leg phenotype of br¹

(Chen et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of LIMK1^{D522A.Scer\UAS} under the control of Scer\GAL4^hs.PB enhances the frequency of the malformed leg phenotype seen in Sb^63b/+ heterozygotes from 8% to 58%. Expression of LIMK1^{D522A.Scer\UAS} under the control of Scer\GAL4^hs.PB enhances the frequency of the malformed leg phenotype seen in Sb⁷⁰/+ heterozygotes from 7% to 84%. Expression of LIMK1^{D522A.Scer\UAS} under the control of Scer\GAL4^hs.PB enhances the frequency of the malformed leg phenotype seen in br¹/Y males from 1% to 36%.

(Chen et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

LIMK1^{D522A.Scer\UAS}

LIMK1^D522A.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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