Expression of Hsap\ESR1Scer\UAS.cKa under the control of Scer\GAL4GMR.PY results in a slight increase in apoptosis in third larval instar eye discs compared to controls. Expression of Hsap\ESR1Scer\UAS.cKa under the control of Scer\GAL4GMR.PY results in a rough eye phenotype.
Hsap\ESR1UAS.cKa, Scer\GAL4GMR.PY has visible phenotype, enhanceable by armΔN.UAS.Tag:HA,Tag:Myr(Unk), Scer\GAL4GMR.PY
Hsap\ESR1UAS.cKa, Scer\GAL4GMR.PY has increased cell death phenotype, enhanceable by armΔN.UAS.Tag:HA,Tag:Myr(Unk), Scer\GAL4GMR.PY
Hsap\ESR1UAS.cKa, Scer\GAL4GMR.PY is an enhancer of visible phenotype of Scer\GAL4GMR.PY, armΔN.UAS.Tag:HA,Tag:Myr(Unk)
Hsap\ESR1UAS.cKa, Scer\GAL4GMR.PY is an enhancer of increased cell death phenotype of Scer\GAL4GMR.PY, armΔN.UAS.Tag:HA,Tag:Myr(Unk)
Hsap\ESR1UAS.cKa, Scer\GAL4GMR.PY has eye disc phenotype, enhanceable by armΔN.UAS.Tag:HA,Tag:Myr(Unk), Scer\GAL4GMR.PY
Hsap\ESR1UAS.cKa, Scer\GAL4GMR.PY has eye phenotype, enhanceable by armΔN.UAS.Tag:HA,Tag:Myr(Unk), Scer\GAL4GMR.PY
Hsap\ESR1UAS.cKa, Scer\GAL4GMR.PY is an enhancer of eye disc phenotype of Scer\GAL4GMR.PY, armΔN.UAS.Tag:HA,Tag:Myr(Unk)
Hsap\ESR1UAS.cKa, Scer\GAL4GMR.PY is an enhancer of eye phenotype of Scer\GAL4GMR.PY, armΔN.UAS.Tag:HA,Tag:Myr(Unk)
Co-expression of armΔ.Scer\UAS.T:Ivir\HA1 and Hsap\ESR1Scer\UAS.cKa under the control of Scer\GAL4GMR.PY results in a marked increase in apoptosis in third larval instar eye discs compared to controls. Treatment with estradiol significantly increases the rate of apoptosis in these double mutant flies. Co-expression of armΔ.Scer\UAS.T:Ivir\HA1 and Hsap\ESR1Scer\UAS.cKa under the control of Scer\GAL4GMR.PY synergistically enhances the rough eye phenotype seen when either is expressed alone. Treatment of the double mutant flies with estrogen further aggravates the severity of the eye phenotype.
Carried in a plasmid and transfected into Schneider S2 cells for transcription assays.