FB2024_03 , released June 25, 2024
Allele: Dmel\nejF2161A.UAS.Tag:V5
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General Information
Symbol
Dmel\nejF2161A.UAS.Tag:V5
Species
D. melanogaster
Name
FlyBase ID
FBal0175573
Feature type
allele
Associated gene
Dmel\nej
Associated Insertion(s)
Carried in Construct
P{UAS-nej.F2161A-V5}
Also Known As
UAS-CBP FL-AD
Key Links
Transgenic product class
missense_variant
(Ludlam et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Ludlam et al., 2002)
Mutagen
in vitro construct
(Kumar et al., 2004, Ludlam et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-nej.F2161A-V5}
Cytology
Description

UASt regulatory sequences drive expression of an acetylase dead version of nej.

(Kumar et al., 2004)

Amino acid replacement: F2161A.

(Ludlam et al., 2002)

UASt regulatory sequences drive expression of a mutated form of nej tagged with Tag:V5.

(Ludlam et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
nej
Tagged with
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Machado-Joseph disease
      modeled by Hsap\ATXN3tr.Q27.UAS.Tag:HA
      (Cho et al., 2022)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of nejF2161A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF produces a roughened eye.

      Expression of nejF2161A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4bbg-C96 produces a notched wing.

      (Boyles et al., 2010)

      Eyes of Scer\GAL4GMR.PF, nejF2161A.Scer\UAS.T:SV5\V5 flies are moderately rough with significant reduction in the number of interommatidial bristles.

      (Mallik and Lakhotia, 2010)

      Overexpression of nejF2161A.Scer\UAS.T:SV5\V5 driven by Scer\GAL4hs.PB does not affect hydroxyurea-mediated cell cycle arrest.

      (Smolik and Jones, 2007)

      Expression of nejF2161A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF results in adults with a mutant eye phenotype; many ommatidia are lacking photoreceptor cells or show gross defects in rhabdomere development. Expression of nejF2161A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4hs.2sev results in defects in ommatidial assembly. Some ommatidia contain two R3 cells (indicating that a putative R4 cell has instead adopted the R3 fate). Very occasionally either the R3 or R4 cell is transformed into an R7 photoreceptor.

      (Kumar et al., 2004)

      Flies expressing nejF2161A.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4GMR.PF have have irregularly arrayed ommatidia and mechanosensory bristles. The ommatidia sometimes contain an abnormal number of photoreceptors.

      (Ludlam et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Coexpression of one copy of P{Sym-UAS-Hsrω} in the Scer\GAL4GMR.PF, nejF2161A.Scer\UAS.T:SV5\V5 background substantially improves eye morphology and ommatidial arrays, whereas coexpression of HsrωEP3037 or HsrωEP93D exaggerates the eye damage.

      (Mallik and Lakhotia, 2010)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of nejF2161A.Scer\UAS.T:SV5\V5 in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 background results in 23% lethality at the pupal stage. Surviving flies show a reduction in eye size and a greater disruption in ommatidial arrays. Additional co-expression of one copy of P{Sym-UAS-Hsrω} improves the eye morphology and size and reduces pupal death, whereas co-expression of HsrωEP3037 or HsrωEP93D results in a greater reduction in eye size, near complete loss of ommatidial arrays and bristles and the appearence of black lesions on the eye surface. Co-expression of HsrωEP3037 also enhances pupal lethality, whereas co-expression of HsrωEP93D does not enhance pupal death.

      Expression of nejF2161A.Scer\UAS.T:SV5\V5 in the Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 background results in 15% lethality at the pupal stage. Surviving flies show a reduction in eye size and a greater disruption in ommatidial arrays. Additional co-expression of one copy of P{Sym-UAS-Hsrω} improves the eye morphology and size and reduces pupal death.

      (Mallik and Lakhotia, 2010)
      Complementation and Rescue Data
      Fails to rescue

      nejF2161A.UAS.Tag:V5/Scer\GAL4hs.PB fails to rescue nejRNAi.UAS.cSa

      (Smolik and Jones, 2007)
      Comments

      Expression of nejF2161A.Scer\UAS.T:SV5\V5 fails to rescue the mitosis phenotypes in eye discs resulting from Scer\GAL4hs.PB-driven nejdsRNA.Scer\UAS expression.

      (Smolik and Jones, 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      nejF2161A.Scer\UAS.T:SV5\V5
      nejF2161A.UAS.Tag:V5
      Name Synonyms
      Secondary FlyBase IDs
        References (9)