FlyBase Allele Report: Dmel\Moe[K.T559D.UAS.Tag:MYC]

General Information

Symbol

Dmel\Moe^{K.T559D.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0176070

Feature type

allele

Associated gene

Dmel\Moe

Associated Insertion(s)

Carried in Construct

P{UAS-Moe.T559D.MYC}

Key Links

Transgenic product class

missense_variant

(Karagiosis and Ready, 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Karagiosis and Ready, 2004)

Mutagen

in vitro construct

(Karagiosis and Ready, 2004)

Progenitor genotype

Moe^{K.UAS.Tag:MYC}

(Karagiosis and Ready, 2004)

Carried in construct

P{UAS-Moe.T559D.MYC}

Cytology

Description

Amino acid replacement: T559D. The primer used to introduce the point mutation (CGTGACAAGTACAAagatctCCGCGAGATTCGTAAGGG) also silently introduced a BglII site (lower case in primer sequence).

(Karagiosis and Ready, 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Moe

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^sca-109-68

(Ruan et al., 2013)

Phenotype Manifest In

apical plasma membrane, with Scer\GAL4^bs.PM

(Schottenfeld-Roames et al., 2014)

lamina plexus | third instar larval stage, with Scer\GAL4^sca-109-68

(Ruan et al., 2013)

medulla | third instar larval stage, with Scer\GAL4^sca-109-68

(Ruan et al., 2013)

rhabdomere & cytoskeleton | apical, with Scer\GAL4^hs.PB

(Karagiosis and Ready, 2004)

rhabdomere & microvillus, with Scer\GAL4^hs.PB

(Karagiosis and Ready, 2004)

rhabdomere & plasma membrane | apical, with Scer\GAL4^hs.PB

(Karagiosis and Ready, 2004)

terminal tracheal cell, with Scer\GAL4^bs.PM

(Schottenfeld-Roames et al., 2014)

tracheal lumen, with Scer\GAL4^bs.PM

(Schottenfeld-Roames et al., 2014)

Detailed Description

Statement

Reference

Tracheal terminal cells expressing the phosphomimetic isoform Moe^{K.T559D.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^bs.PM exhibit abnormal dilations of the lumenal membrane and reduced branch number.

(Schottenfeld-Roames et al., 2014)

The lamina plexus is uneven and irregular, thick axons bundles are seen in the medulla in third instar larvae expressing Moe^{K.T559D.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^sca-109-68.

(Ruan et al., 2013)

When Moe^{K.T559A.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^hs.PB pupae are heat shocked during apical morphogenesis of the rhabdomeres, microvilli at the apical surface of rhabdomeres become irregular: the apical membrane between adherens junctions is severely disorganized in these cells, as is the apical cytoskeleton.

(Karagiosis and Ready, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Moe^{K.T559D.UAS.Tag:MYC}/Scer\GAL4^332.3 is a suppressor | partially of majority die during embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Moe^{K.T559D.UAS.Tag:MYC}/Scer\GAL4^332.3 is a suppressor | partially of embryo | dorsal closure stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Moe^{K.T559D.UAS.Tag:MYC}/Scer\GAL4^332.3 is a suppressor | partially of embryonic/first instar larval cuticle | embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Moe^{K.T559D.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^332.3 partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background.

(Flores-Benitez and Knust, 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8630

w¹¹¹⁸; P{UAS-Moe.T559D.MYC}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DMoe^T559D

(Flores-Benitez and Knust, 2015)

Moe^{K.T559A.Scer\UAS.T:Hsap\MYC}

Moe^{K.T559D.Scer\UAS.T:Hsap\MYC}

Moe^{K.T559D.UAS.Tag:MYC}

Moe^TD

(Plutoni et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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