FlyBase Allele Report: Dmel\Moe[RNAi.327-775.UAS]

General Information

Symbol

Dmel\Moe^{RNAi.327-775.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0176074

Feature type

allele

Associated gene

Dmel\Moe

Associated Insertion(s)

Carried in Construct

P{UAS-Moe.RNAi.327-775}

Also Known As

UAS-MoeRNAi

Key Links

Transgenic product class

RNAi_reagent

(Karagiosis and Ready, 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Karagiosis and Ready, 2004)

Mutagen

in vitro construct

(Karagiosis and Ready, 2004)

Progenitor genotype

Carried in construct

P{UAS-Moe.RNAi.327-775}

Cytology

Description

UAS sequences control expression of an inverted repeat of 327-775 of Moe (numbering as for GenBank: L38909).

(Karagiosis and Ready, 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Moe

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

viable, with Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

visible, with Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Phenotype Manifest In

1st posterior cell, with Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

cyst progenitor cell, with Scer\GAL4^C587

(Cheng et al., 2011)

photoreceptor neuron, with Moe^{RNAi.818-1326.UAS}, Scer\GAL4^GMR.PF

(Karagiosis and Ready, 2004)

rhabdomere & actin filament, with Moe^{RNAi.818-1326.UAS}, Scer\GAL4^GMR.PF

(Karagiosis and Ready, 2004)

rhabdomere & actin filament, with Moe^{RNAi.818-1326.UAS}, Scer\GAL4^hs.PB

(Karagiosis and Ready, 2004)

spindle, with Scer\GAL4^C587

(Cheng et al., 2011)

wing, with Moe^{RNAi.818-1326.UAS}, Scer\GAL4^MD-638

(Molnar and de Celis, 2006)

wing blade, with Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

wing disc, with Moe^{RNAi.818-1326.UAS}, Scer\GAL4^MD-638

(Molnar and de Celis, 2006)

wing disc | third instar larval stage, with Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

wing hinge, with Moe^{RNAi.818-1326.UAS}, Scer\GAL4^MD-638

(Molnar and de Celis, 2006)

wing vein, with Moe^{RNAi.818-1326.UAS}, Scer\GAL4^MD-638

(Molnar and de Celis, 2006)

Detailed Description

Statement

Reference

Only 18% of testis somatic cyst stem cells show repositioning of the mitotic spindle during anaphase in males expressing Moe^{dsRNA.327-775.Scer\UAS} under the control of Scer\GAL4^C587 compared to 76% of cases in heterozygous controls.

(Cheng et al., 2011)

Expression of Moe^{dsRNA.327-775.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 induces ectopic apoptosis in third instar wing imaginal disc cells.

Wings form Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS} adults display a reduced area between veins 3 and 4.

(Neisch et al., 2010)

Moe^{818-1326.dsRNA.Scer\UAS}; Moe^{327-775.dsRNA.Scer\UAS}; Scer\GAL4^MD-638 flies have wing phenotypes similar to those due to Moe^c858 : wings are small (in particular, they are severely shortened along the proximal-distal axis); vesicles form between the dorsal and ventral surfaces of the wing; veination is defective. In the late third instar wing disc, there is ectopic folding of the disc epithelium. This folding sometimes extends back under the disc making transverse sections multi-layered.

(Molnar and de Celis, 2006)

Rhabdomeres in the eyes of Moe^{818-1326.dsRNA.Scer\UAS}; Moe^{327-775.dsRNA.Scer\UAS}; Scer\GAL4^hs.PB flies heat shocked at approximately 50% pupal development have a disorganised actin cytoskeleton and reduced, irregular microvillar fields. Apical differentiation and organisation of the actin cytoskeleton is severely disrupted in the photoreceptors of Moe^{818-1326.dsRNA.Scer\UAS}; Moe^{327-775.dsRNA.Scer\UAS}; Scer\GAL4^GMR.PF animals. Microvillar and stalk membranes in these photoreceptors are distinct but jumbled. Despite these defects, epithelial integrity is maintained, and photoreceptor adherens-junctions appear normal.

(Karagiosis and Ready, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1 has visible phenotype, enhanceable by puc^E69/puc[+], Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Suppressed by

Statement

Reference

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1 has increased cell death phenotype, suppressible by Rho1^72R/Rho1[+], Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by Rho1^72R/Rho1[+]

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by hep^NIG.4353R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by slpr^NIG.2272R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by Tak1^NIG.1388R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by Tak1^K46R.M.UAS, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1 has increased cell death phenotype, suppressible by Rho1^E3.10/Rho1[+], Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by POSH^GD11467, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

NOT suppressed by

Statement

Reference

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, non-suppressible by Ask1^K618M.UAS, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1 has wing blade phenotype, enhanceable by puc^E69/puc[+], Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Suppressed by

Statement

Reference

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1 has wing disc | third instar larval stage phenotype, suppressible by Rho1^72R/Rho1[+], Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by Rho1^72R/Rho1[+], Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by hep^NIG.4353R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by slpr^NIG.2272R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by Tak1^NIG.1388R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by Tak1^K46R.M.UAS, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1 has wing disc | third instar larval stage phenotype, suppressible by Rho1^E3.10/Rho1[+], Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by POSH^GD11467, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

NOT suppressed by

Statement

Reference

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, non-suppressible by Ask1^K618M.UAS, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Rho1^72R/+ suppresses the ectopic apoptosis in wing disc cells expressing Moe^{dsRNA.327-775.Scer\UAS} under the control of Scer\GAL4^dpp.blk1.

Heterozygous puc^E69 mutant flies expressing Moe^{dsRNA.327-775.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 exhibit a severely reduced or rudimentary wing phenotype.

Rho1^72R/+ strongly suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Co-expression of hep^{dsRNA.Scer\UAS} suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Co-expression of slpr^{dsRNA.Scer\UAS.NIG} suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Co-expression of Tak1^{dsRNA.Scer\UAS} suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Co-expression of Tak1^{K46R.M.Scer\UAS} suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Co-expression of Pk92B^{K618M.Scer\UAS} has no effect on the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Rho1^E3.10/+ suppresses the ectopic apoptosis in wing disc cells expressing Moe^{dsRNA.327-775.Scer\UAS} under the control of Scer\GAL4^dpp.blk1.

Co-expression of POSH^GD11467 suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

(Neisch et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8629

w¹¹¹⁸; P{UAS-Moe.RNAi.327-775}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Moe^{327-775.dsRNA.Scer\UAS}

Moe^{RNAi.327-775.UAS}

Moe^{dsRNA.327-775.Scer\UAS}

Moe^{dsRNA.327-775.UAS}

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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