In Pxt^f01000/Pxt^f01000 females, both anterior and posterior nurse cells have a greatly enlarged and fragmented nucleolus lacking interconnected tubules during oogenesis, with a greater severity at stage 10B than at stages 8, 9 and 10A. Additionally, their inability to form cytoplasmic actin bundles and to complete dumping by stage 13, results in larger nurse cells and a smaller oocyte than controls. Nurse cells further exhibit an excess of lipid droplets in the cytoplasm during oogenesis.

Homozygous pxt^f01000 females are sterile.

pxt^f01000 mutant follicles frequently show defects in nurse cell dumping.

pxt^f01000 mutant ovarioles typically contain fewer egg chambers than wild-type ones. These mutant ovarioles are deficient in maturing follicles. Approximately 25% of pxt^f01000 ovarioles contain a fusion of two adjacent egg chambers and in about half of the ovarioles there is at least one degenerating follicle.

pxt^f01000 nurse cell plasma membranes are unstable and frequently break down to produce multinucleate nurse cells in which an actin-rich aggregate containing ring canal remnants remains. Membrane breakdown preferentially occurs in anterior nurse cells. The membrane breakdown, fusion and degeneration defects increase with age, because in 25-day-old pxt^f01000 females, the ovarioles are reduced to a single fused structure.

In both homozygotes and trans-heterozygotes of pxt^f01000 and pxt[EY03052], more than 50% of stage 9-10 border cell clusters do not complete migration completely.

Pxt^f01000 has nurse cell | oogenesis phenotype, suppressible by sn^UASp.EGFP/Scer\GAL4^VP16.osk

Pxt^f01000 has actin filament bundle | oogenesis phenotype, suppressible by sn^UASp.EGFP/Scer\GAL4^VP16.osk

Pxt^f01000 has nucleolus | oogenesis phenotype, suppressible by sn^UASp.EGFP/Scer\GAL4^VP16.osk

Pxt^f01000 has stretch follicle cell phenotype, suppressible by Mmus\Ptgs1^UASp.cTa/Scer\GAL4^hs.PB