FB2024_03 , released June 25, 2024
Allele: Dmel\snUASp.EGFP
Open Close
General Information
Symbol
Dmel\snUASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0239091
Feature type
allele
Associated gene
Dmel\sn
Associated Insertion(s)
Carried in Construct
P{UASp-sn.EGFP}
Also Known As
GFP-Fascin, Fascin-GFP
Key Links
Transgenic product class
cDNA
(Zanet et al., 2009)
wild_type
(Zanet et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Zanet et al., 2009)
wild_type
(Zanet et al., 2009)
Mutagen
in vitro construct
(Zanet et al., 2009)
Progenitor genotype
Carried in construct
P{UASp-sn.EGFP}
Cytology
Description

UASp regulatory sequences drive expression of a full-length sn cDNA (RH62992) with EGFP fused to the N-terminus.

(Zanet et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
sn
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      snUASp.EGFP/Scer\GAL4VP16.osk rescues sn28

      (Groen et al., 2015)

      snUASp.EGFP/Scer\GAL4sn.PZ rescues Df(1)C128

      (Zanet et al., 2012)

      snUASp.EGFP/Scer\GAL4VP16.nanos.UTR rescues sn28

      (Zanet et al., 2012)

      snUASp.EGFP/Scer\GAL4sn.PZ rescues sn28

      (Zanet et al., 2012)

      snUASp.EGFP/Scer\GAL4srp.Hemo rescues Df(1)C128

      (Zanet et al., 2009)

      snUASp.EGFP/Scer\GAL4VP16.nanos.UTR rescues sn28

      (Zanet et al., 2009)

      snUASp.EGFP/Scer\GAL4hs.PB rescues sn3

      (Zanet et al., 2009)
      Partially rescues

      snUASp.EGFP/Scer\GAL4srp.Hemo partially rescues snX2

      (Zanet et al., 2009)
      Comments

      Expressing snUASp.EGFP under the control of Scer\GAL4VP16.osk rescues the disperse nucleolar phenotype in sn28/sn28 nurse cells.

      (Groen et al., 2015)

      Expression of snScer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4sn.PZ rescues the macrophage migration defects seen in sn28 mutant stage 15 embryos. The reduction in the number of actin rich filopodia and microspikes within the lamellae of sn28 mutant macrophages is also rescued.

      Expression of snScer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the female sterility and actin bundle formation defects seen in sn28 mutant nurse cells.

      Expression of snScer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4sn.PZ rescues the macrophage migration defects seen in Df(1)C128 mutant stage 15 embryos.

      (Zanet et al., 2012)

      Scer\GAL4srp.Hemo-driven expression of snScer\UAS.P\T.T:Avic\GFP-EGFP restores the polarity and the dynamic leading edge cortex that is normally lacking in plasmatocytes in Df(1)C128 embryos. Normal developmental migration of plasmatocytes is also restored.

      Scer\GAL4srp.Hemo-driven expression of snScer\UAS.P\T.T:Avic\GFP-EGFP provides significant rescue of the reduced plasmatocyte recruitment seen in snX2 embryos after laser-induced wounding.

      Scer\GAL4nos.UTR.T:Hsim\VP16-driven expression of snScer\UAS.P\T.T:Avic\GFP-EGFP fully restores the formation of actin bundles around nurse cell nuclei that is normally lacking in sn28 mutants. Female ferility is also restored.

      Scer\GAL4hs.PB-driven expression of snScer\UAS.P\T.T:Avic\GFP-EGFP fully rescues the gnarled bristle phenotype exhibited by sn3 mutants.

      (Zanet et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      snScer\UAS.P\T.T:Avic\GFP-EGFP
      snUASp.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (9)