FlyBase Allele Report: Dmel\ft[UAS.cMa]

General Information

Symbol

Dmel\ft^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Matakatsu

FlyBase ID

FBal0179776

Feature type

allele

Associated gene

Dmel\ft

Associated Insertion(s)

Carried in Construct

P{UAS-ft.M}

Also Known As

UAS-ft, UAS.ft, UAS-fat, ft^Scer\UAS.cMa

Key Links

Transgenic product class

wild_type

(Matakatsu and Blair, 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Matakatsu and Blair, 2004)

Mutagen

in vitro construct

(Matakatsu and Blair, 2004)

Progenitor genotype

Carried in construct

P{UAS-ft.M}

Cytology

Description

Expression of ft is driven by UASt sequences.

(Matakatsu and Blair, 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

denticle row 1, with Scer\GAL4^ptc-559.1

(Repiso et al., 2010)

microtubule, with Scer\GAL4^en-e16E

(Harumoto et al., 2010)

ommatidium | adult stage | somatic clone, with Scer\GAL4^Act5C.PI

(Brittle et al., 2012)

tergite, with Scer\GAL4^en-e16E

(Repiso et al., 2010)

trichome & abdominal tergite | somatic clone | cell non-autonomous, with Scer\GAL4^αTub84B.PL

(Casal et al., 2006)

ventral denticle belt, with Scer\GAL4^ptc-559.1

(Repiso et al., 2010)

wing, with Scer\GAL4^Act5C.PI

(Matakatsu and Blair, 2006)

wing, with Scer\GAL4^en-e16E

(Repiso et al., 2010)

wing blade, with Scer\GAL4^Bx-MS1096

(Hogan et al., 2011)

wing hair, with Scer\GAL4^Bx-MS1096

(Hogan et al., 2011)

wing hair, with Scer\GAL4^en-e16E

(Harumoto et al., 2010)

Detailed Description

Statement

Reference

Overexpression of ft^Scer\UAS.cMa under the control of Scer\GAL4^Bx-MS1096 affect wing shape and alters posterior wing ridges. ft^Scer\UAS.cMa overexpression alters wing hair polarity in the proximal region as well as it also generates variable hair polarity changes in more distal regions of the wing.

Overexpression of ft^Scer\UAS.cMa under the control of Scer\GAL4^salm-459.2 does not affect wing hair polarity.

(Hogan et al., 2011)

Expression of ft^Scer\UAS.cMa under the control of Scer\GAL4^en-e16E in the posterior compartment of the wing results in misorientation of wing hairs within the expression domain. These animals show defects in the directional preferences of growing microtubules in wing cells 24 hours after puparium formation; microtubule polarity in the proximal region of the wing is reversed, with a significant excess of plus-end proximal microtubules compared to wild type. The proximodistal alignment of microtubules becomes less strong compared to wild type.

(Harumoto et al., 2010)

Generalized overexpression of ft^Scer\UAS.cMa under the control of Scer\GAL4^arm.PS has little effect on denticle belt polarity.

Overexpression of ft^Scer\UAS.cMa in the P compartment (under the control of Scer\GAL4^en-e16E) causes 'whorly' polarity in the P compartment of the wing and tergites. Expression in larvae results, in some cases, in row 1 cells making clusters of bristles instead of rows of normally oriented denticles. Overexpression under the control of Scer\GAL4^ptc-559.1 in the region of the denticles does not result in changes in polarity.

(Repiso et al., 2010)

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL in a wild-type background result in a reversal of cell polarity in wild-type cells anterior to the clone (hairs in front the clone point forwards).

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL in a ft¹²/ft^unspecified background do not change the cell polarity of cells surrounding the clone.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are mutant for ft^unspecified and are also expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL result in a reversal of cell polarity in cells anterior to the clone.

(Casal et al., 2006)

Following overexpression of ft^Scer\UAS.cMa, under the control of Scer\GAL4^e22c, in the posterior wing compartment, cells flanking the DV boundary display wider apical circumferences when compared with those of the anterior wing compartment. Wing growth along the proximal-distal axis in the posterior compartment is truncated, as revealed by the close proximity of the anterior and posterior crossveins.

(Jaiswal et al., 2006)

S2 cells that express ft^Scer\UAS.cMa, under the control of Scer\GAL4^Act5C.PI, aggregate.

Expression of ft^Scer\UAS.cMa under the control of Scer\GAL4^Act5C.PI causes mild perturbation of planar cell polarity in the wing and a foreshortening along the proximodistal axis of the wing blade as the anterior and posterior crossveins are abnormally close.

(Matakatsu and Blair, 2006)

ft^Scer\UAS.cMa; Scer\GAL4^Act5C transfected S2 cells do not aggregate.

(Matakatsu and Blair, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^αTub84B.PL, ds^UAO71, ft¹⁵, ft^UAS.cMa has abnormal planar polarity | cell non-autonomous | somatic clone phenotype, enhanceable | somatic clone by fj^UAS.cZa/Scer\GAL4^αTub84B.PL

(Brittle et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, ft^UAS.cMa has abnormal planar polarity phenotype, suppressible by Scer\GAL4^en-e16E/par-1^RNAi.UAS.cZa

(Harumoto et al., 2010)

Scer\GAL4^en-e16E, ft^UAS.cMa has abnormal planar polarity phenotype, suppressible | partially by fj^d1/fj^d1

(Matakatsu and Blair, 2004)

Other

Statement

Reference

Scer\GAL4^Bx-MS1096, ft^UAS.cMa, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^Bx-MS1096, ft^UAS.cMa, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ft^UAS.cMa, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ft^UAS.cMa, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Scer\GAL4^αTub84B.PL, ds^UAO71, ft¹⁵, ft^UAS.cMa has abnormal planar polarity | somatic clone | cell non-autonomous phenotype

(Brittle et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^αTub84B.PL, ds^UAO71, ft¹⁵, ft^UAS.cMa has trichome & tergite | cell non-autonomous | somatic clone phenotype, enhanceable | somatic clone by fj^UAS.cZa/Scer\GAL4^αTub84B.PL

(Brittle et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, ft^UAS.cMa has wing hair phenotype, suppressible by Scer\GAL4^en-e16E/par-1^RNAi.UAS.cZa

(Harumoto et al., 2010)

Scer\GAL4^en-e16E, ft^UAS.cMa has wing hair | posterior phenotype, suppressible by fj^d1/fj^d1

(Matakatsu and Blair, 2004)

Scer\GAL4^en-e16E, ft^UAS.cMa has wing blade posterior compartment phenotype, suppressible by fj^d1/fj^d1

(Matakatsu and Blair, 2004)

Other

Statement

Reference

Scer\GAL4^Bx-MS1096, ft^UAS.cMa, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ft^UAS.cMa, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Scer\GAL4^αTub84B.PL, ds^UAO71, ft¹⁵, ft^UAS.cMa has trichome & tergite | somatic clone | cell non-autonomous phenotype

(Brittle et al., 2010)

Scer\GAL4^Act5C.PU, ds^UAS.cTa, ft^UAS.cMa has S2 cell-line phenotype

(Matakatsu and Blair, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

When ft^Scer\UAS.cMa is overexpressed under the control of Scer\GAL4^Bx-MS1096 in a pk³⁰ mutant wing, the pk³⁰ wing hair phenotype is modified to a more distal polarity in the anterior and distal posterior regions.

Overexpression of ft^Scer\UAS.cMa under the control of Scer\GAL4^salm-459.2 in a pk³⁰ mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4^salm-459.2 expression domain.

(Hogan et al., 2011)

ds^UAO71 ft¹⁵ double mutant clones in the dorsal adult abdomen where the clones are also expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL cause repolarisation in front of the clones, so that hairs point away from the clone (64% of clones repolarise with a maximum range of seven cells). Co-expression of fj^Scer\UAS.cZa within these clones strengthens the repolarisation (95% of clones repolarise with a maximum range of seven cells).

(Brittle et al., 2010)

The planar polarity defects seen in the wing in animals expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^en-e16E is significantly suppressed by co-expression of par-1^{dsRNA.Scer\UAS.cZa}.

(Harumoto et al., 2010)

The reversal of cell polarity that is seen in cells anterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL is still seen if the clones are induced in a stan^- background (the cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59).

The reversal of cell polarity that is seen in cells anterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL is still seen if the clones are induced in a fz^unspecified stan^- background (cells within the clone are stan^E59/stan^E59 while the cells surrounding the clone are stan³/stan^E59).

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are mutant for fz^unspecified and are also expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL result in reversal of cell polarity in cells posterior to the clone when the clone is at the front of the anterior compartment (behaving like fz^- clones) and result in reversal of cell polarity in cells anterior to the clone when the clone is at the back of the anterior compartment (behaving like clones expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL).

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) that are expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL in a ds^unspecified background do not change the cell polarity of cells surrounding the clone.

Clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are doubly mutant for ds^unspecified and ft^unspecified and are also expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL can reverse the cell polarity of cells anterior to the clone.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are co-expressing both ft^Scer\UAS.cMa and ds^Scer\UAS.cTa under the control of Scer\GAL4^αTub84B.PL result in a reversal of cell polarity in cells anterior to the clone.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are co-expressing both ft^Scer\UAS.cMa and ds^{ecto.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL result in a reversal of cell polarity in cells anterior to the clone.

The reversal of cell polarity that is seen in cells anterior to clones in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL is enhanced if the clones are induced in a fj^unspecified background compared to a wild-type background; the repolarisation occurs over a greater range in the fj^unspecified background.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are co-expressing both fz^Scer\UAS.cUa and ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL and the clones have been induced in a ds^unspecified background, result in reversal of cell polarity in cells anterior to the clone.

Clones of cells in the anterior compartment of the dorsal epidermis of the adult abdomen (in the tergite) where the clones are co-expressing both fz^Scer\UAS.cUa and ft^Scer\UAS.cMa under the control of Scer\GAL4^αTub84B.PL and the clones have been induced in a ft¹²/ft^unspecified background, result in reversal of cell polarity in cells anterior to the clone.

(Casal et al., 2006)

S2 cells that express ft^Scer\UAS.cMa and ds^Scer\UAS.cTa, under the control of Scer\GAL4^Act5C.PI, aggregate, as do cells co-transfected with ft^Scer\UAS.cMa and ds^{ΔICD.Scer\UAS}.

(Matakatsu and Blair, 2006)

S2 cells co-transfected with ds^Scer\UAS.cTa, ft^Scer\UAS.cMa and Scer\GAL4^Act5C aggregate into clusters of about 50 cells. This aggregation occurs even if 2 groups of cells are transfected separately with ds^Scer\UAS.cTa; Scer\GAL4^Act5C or ft^Scer\UAS.cMa; Scer\GAL4^Act5C and then mixed. Formation of these aggregates is inhibited by the presence of EGTA. Co-transfection of fj^Scer\UAS.cZa with ds^Scer\UAS.cTa, ft^Scer\UAS.cMa and Scer\GAL4^Act5C into S2 cells has no detectable effect on their aggregation, or the sensitivity of aggregates to agitation. Planar cell polarity defects in the posterior compartment of ft^Scer\UAS.cMa; Scer\GAL4^en-e16E flies are weakly suppressed by ds⁰⁵¹⁴²/ds⁰⁵¹⁴² and strongly suppressed by fj^d1/fj^d1.

(Matakatsu and Blair, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

ft^UAS.cMa/Scer\GAL4^da.G32 rescues ft⁸/ft^G-rv

(Matakatsu and Blair, 2006)

Partially rescues

ft^UAS.cMa/Scer\GAL4^Act.PU partially rescues ft⁸/ft^G-rv

(Matakatsu and Blair, 2012)

ft^UAS.cMa/Scer\GAL4^Act5C.PI partially rescues ft⁸/ft^G-rv

(Matakatsu and Blair, 2006)

ft^UAS.cMa/Scer\GAL4^en-e16E partially rescues ft⁸/ft^G-rv

(Matakatsu and Blair, 2006)

ft^UAS.cMa/Scer\GAL4^da.G32 partially rescues ft⁸/ft^G-rv

(Matakatsu and Blair, 2004)

Comments

Expression of ft^Scer\UAS.cMa under the control of Scer\GAL4^Act.PU in ft^G-rv/ft⁸ animals substantially improves hair planar cell polarity defects in the abdomen.

(Matakatsu and Blair, 2012)

Expression of ft^Scer\UAS.cMa in the posterior wing pouch, driven by Scer\GAL4^en-e16E, rescues the overgrowth phenotype of ft^G-rv/ft⁸ mutants in this region, but does not rescue overgrowth in the anterior.

Expression of ft^Scer\UAS.cMa under the control of Scer\GAL4^Act5C.PI rescues the lethality and wing disc overgrowth phenotype of ft^G-rv/ft⁸ mutants and partially rescues the abdominal planar cell polarity defect.

(Matakatsu and Blair, 2006)

ft^Scer\UAS.cMa; Scer\GAL4^da.G32 rescues the pupal lethality of ft^G-rv/ft⁸ and substantially rescues planar cell polarity and tarsal segment defects in these animals.

(Matakatsu and Blair, 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2 cells for use in cell aggregation assays.

(Matakatsu and Blair, 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ft^Scer\UAS.cMa

(Brittle et al., 2010, Casal et al., 2006)

ft^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Matakatsu

(Matakatsu and Blair, 2004)

Secondary FlyBase IDs

References (18)

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