adult head & macrochaeta, with Scer\GAL4sca.PC
eye, with Scer\GAL4ey.PH
larval neuromuscular junction & bouton, with Scer\GAL4elav-C155
photoreceptor cell & growth cone & larval brain, with Scer\GAL4ey.PH
trichogen cell & actin filament & pupa, with Scer\GAL4sca.PC
About 10% of the heads of Sra-1dsRNA.Scer\UAS; Scer\GAL4sca.PC flies have one or more bent macrochaetae. This phenotype is prefigured by the formation of bent actin filament bundles at the sites of macrochaeta development in pupae. Sra-1dsRNA.Scer\UAS; Scer\GAL4ey.PH have severe axonal targeting defects: Most axons originating in the eye disc enter the brain but fail to grow towards their correct targets. Fewer photoreceptor cells develop in the eye discs of these animals. The resulting adult eyes are small and rough. The boutons of neuromuscular junctions in Sra-1dsRNA.Scer\UAS; Scer\GAL4elav-C155 third instar larvae exhibit increased budding of boutons. About 10% of the heads of Sra-1dsRNA.Scer\UAS; Scer\GAL4sca.PC flies have one or more bent macrochaetae. When 2 copies of the Sra-1dsRNA.Scer\UAS transgene are present the penetrance of this phenotype inceases to about 30%, while about 5% lack one or more head bristles.
CyfipRNAi.UAS, Scer\GAL4sca.PC has macrochaeta phenotype, enhanceable by Hemunspecified
The penetrance of the bent macrochaeta phenotype seen in the heads of Sra-1dsRNA.Scer\UAS; Scer\GAL4sca.PC flies is enhanced by Hemunspecified/+.
dsRNA expressed under the control of Scer\UAS sequences is used to study the phenotypic consequences of dsRNA interference (RNAi) of Sra-1.