FlyBase Allele Report: Dmel\Gαq[Q203L.UAS]

General Information

Symbol

Dmel\Gαq^Q203L.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0183500

Feature type

allele

Associated gene

Dmel\Gαq

Associated Insertion(s)

Carried in Construct

P{UAS-Gαq.Q203L}

Also Known As

UAS-AcGq3

Key Links

Transgenic product class

missense_variant

(Ratnaparkhi et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Ratnaparkhi et al., 2002)

Mutagen

in vitro construct

(Ratnaparkhi et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Gαq.Q203L}

Cytology

Description

UASt regulatory sequences drive expression of a mutated form of the Gαq 3 splice variant (carries the amino acid replacement Q203L), resulting in constitutive activation of Gαq.

(Ratnaparkhi et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Gαq

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ap-md544

(Ratnaparkhi et al., 2002)

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Ratnaparkhi et al., 2002)

abnormal neuroanatomy, with Scer\GAL4^ftz.ng

(Ratnaparkhi et al., 2002)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^15J2

(Hiramoto and Hiromi, 2006)

abnormal neurophysiology, with Scer\GAL4^hs.PB

(Kain et al., 2008)

abnormal neurophysiology | heat sensitive, with Scer\GAL4^hs.PU

(Yao and Carlson, 2010)

abnormal sensory perception, with Scer\GAL4^Gr21a.9.323

(Yao and Carlson, 2010)

abnormal smell perception, with Scer\GAL4^VP16.Orco

(Yao and Carlson, 2010)

abnormal smell perception | heat sensitive, with Scer\GAL4^hs.PU

(Yao and Carlson, 2010)

majority die during larval stage, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

partially lethal - majority die, with Scer\GAL4^phtm.PO

(Yamanaka et al., 2015)

visible | adult stage | light conditional | progressive, with Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

Phenotype Manifest In

adult olfactory receptor neuron Gr21a/63a, with Scer\GAL4^Gr21a.9.323

(Yao and Carlson, 2010)

adult olfactory receptor neuron Gr21a/63a | heat sensitive, with Scer\GAL4^hs.PU

(Yao and Carlson, 2010)

adult olfactory receptor neuron Or22, with Scer\GAL4^VP16.Orco

(Yao and Carlson, 2010)

adult olfactory receptor neuron Or42b, with Scer\GAL4^VP16.Orco

(Yao and Carlson, 2010)

adult olfactory receptor neuron Or59b, with Scer\GAL4^VP16.Orco

(Yao and Carlson, 2010)

dMP2 neuron, with Scer\GAL4^15J2

(Hiramoto and Hiromi, 2006)

fascicle | embryonic stage, with Scer\GAL4^ap-md544

(Ratnaparkhi et al., 2002)

fascicle | embryonic stage, with Scer\GAL4^elav-C155

(Ratnaparkhi et al., 2002)

fascicle | embryonic stage, with Scer\GAL4^ftz.ng

(Ratnaparkhi et al., 2002)

neuropil | embryonic stage, with Scer\GAL4^elav-C155

(Ratnaparkhi et al., 2002)

ommatidium | light conditional, with Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

pCC neuron, with Scer\GAL4^ftz.ng

(Ratnaparkhi et al., 2002)

rhabdomere | decreased number | light conditional, with Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

symmetrical commissure | embryonic stage, with Scer\GAL4^elav-C155

(Ratnaparkhi et al., 2002)

Detailed Description

Statement

Reference

Adults expressing Gαq^Q203L.UAS under the control of Scer\GAL4^ninaE.PT show light-dependent retinal degeneration i.e. progressive decrease in the number of rhabdomeres per ommatidium.

(Kumari et al., 2022)

Expression of Gαq^{Q203L.Scer\UAS} in the prothoracic gland (driven by Scer\GAL4^phm.PO) leads to early larval lethality (less than 5% develop to L3 or beyond).

(Yamanaka et al., 2015)

Flies expressing constitutively active Gαq^{Q203L.Scer\UAS} under the control of Scer\GAL4^Orco.PK show alterations in the response amplitudes to cyclohexanol and to low concentrations of benaldehyde, but not to ethyl acetate and higher benzaldehyde concentration.

(Deng et al., 2011)

Expression of Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^Gr21a.9.323 almost abolishes the response of the ab1C olfactory receptor neuron to CO[[2]] at all tested CO[[2]] concentrations.

The odour responses of the ab1A, ab2A and ab3A olfactory receptor neurons are reduced compared to wild type in animals expressing Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^{Orco.T:Hsim\VP16}.

Transient expression of Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^hs.PU using heat shock results in a threefold increase in the spontaneous firing rate of ab1C ORNs compared to non-heat shocked controls. The spontaneous firing rate of ab3A ORNs is unaffected in the heat shocked flies. Transient expression of Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^hs.PU using heat shock does not affect the odour response of the ab3A neurons, but the response of ab1C neurons to CO[[2]] is reduced compared to controls.

Expression of Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^Gr21a.9.323 selectively in adulthood (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to block expression at earlier stages of development) is sufficient to decrease the response to CO[[2]] compared to wild type, at all concentrations of CO[[2]] tested.

(Yao and Carlson, 2010)

Expression of Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^hs.PB at 4-5 days after eclosion (using a heat shock of 15 or 30 minutes) results in electroantennograms with an increased amplitude compared to controls in response to ethyl acetate or propionic acid. If these flies are instead heat shocked for 1 hour to induce expression of Gα49B^{Q203L.Scer\UAS}, no increase in the electroantennogram amplitude is seen relative to control flies.

(Kain et al., 2008)

Expression of Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^15J2 in dMP2 neurons results in its growth cone having an arbor-like structure. If expression is induced after the dMP2 neuron has made its initial posterior turn, the dMP2 axons generate roundabout-like contralateral extensions.

(Hiramoto and Hiromi, 2006)

In embryos expressing Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^elav-C155, the pattern of the central nervous system fascicles and commissures appears 'mildly' deranged in that the commissures are thicker and the neuropil region is broader than in wild-type, when visualised with BP102. In embryos expressing Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^elav-C155, the pattern of the central nervous system appears abnormal, with variable expressivity, when visualised with Fas2. At late stage 13, 'Stalling', where projections are delayed and minute outgrowths are visible from the cell body and axonal tract, is visible in 34.41% of hemisegments from throughout the embryo in stages 14-16. From stage 15 onwards, a proportion of Fas2-expressing axons cross the midline. This is observed in 64/133 abdominal segments. Occasionally a whirling phenotype similar to that observed in robo mutant alleles is visible. In embryos expressing Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^elav-C155, axons from ap-expressing neurons no longer remain on the ipsilateral side but cross the midline. However, unlike axons that crossover in robo mutant embryos, these appear to stall after reaching and crossing the midline. In embryos expressing Gα49B^{Q203L.Scer\UAS} under the regulation of Scer\GAL4^ftz.ng, midline crossing of Fas2-expressing neurons, which do not cross the midline in wild-type embryos, can be observed. At stage 13, the pCC axon, which normally projects anteriorly on the ipsilateral side, turns towards the midline in embryos expressing Gα49B^{Q203L.Scer\UAS} under the regulation of Scer\GAL4^ftz.ng. 'Stalling', where projections are delayed and minute outgrowths are visible from the cell body and axonal tract, is visible in 20.02% of hemisegments from throughout the embryo in stages 14-16. From stage 15 onwards, a proportion of Fas2-expressing axons cross the midline. This is observed in 86/427 abdominal segments. Similar results are observed when Gα49B^{Q203L.Scer\UAS} is expressed under the control of Scer\GAL4^eve.RRC. The pCC axon crosses the midline, whereas the aCC and RP2 projections appear wild-type. In embryos expressing Gα49B^{Q203L.Scer\UAS} under the regulation of Scer\GAL4^ap-md544, ap-expressing dorsal cells also change their trajectory compared to wild-type. Instead of projecting towards the anterior and in an ipsilateral direction as in wild-type, a fraction of the axons are observed 'drifting' across the midline. Gα49B^{Q203L.Scer\UAS};Scer\GAL4^ftz.ng embryos exhibit midline crossing in 86/427 abdominal segments.

(Ratnaparkhi et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gαq^Q203L.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy phenotype, enhanceable by robo[+]/robo1¹

(Ratnaparkhi et al., 2002)

Suppressed by

Statement

Reference

Gαq^Q203L.UAS, Scer\GAL4^ninaE.PT has visible | adult stage | progressive | light conditional phenotype, suppressible | partially by PIP5K59B^{RNAi.UAS.L.cKa}, Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

Gαq^Q203L.UAS, Scer\GAL4^phtm.PO has majority die during larval stage phenotype, suppressible | partially by Itpr^GD1676

(Yamanaka et al., 2015)

Gαq^Q203L.UAS, Scer\GAL4^phtm.PO has majority die during larval stage phenotype, suppressible | partially by stac^GD7394

(Yamanaka et al., 2015)

Gαq^Q203L.UAS, Scer\GAL4^phtm.PO has majority die during larval stage phenotype, suppressible | partially by Rab3^KK108633

(Yamanaka et al., 2015)

Gαq^Q203L.UAS, Scer\GAL4^phtm.PO has majority die during larval stage phenotype, suppressible | partially by Syt1^KK108653

(Yamanaka et al., 2015)

Gαq^Q203L.UAS, Scer\GAL4^15J2 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by fra³

(Hiramoto and Hiromi, 2006)

Gαq^Q203L.UAS, Scer\GAL4^15J2 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9

(Hiramoto and Hiromi, 2006)

Gαq^Q203L.UAS, Scer\GAL4^15J2 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by robo1^UAS.Tag:MYC, Scer\GAL4^15J2

(Hiramoto and Hiromi, 2006)

Gαq^Q203L.UAS, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, suppressible by fra³/fra⁴

(Ratnaparkhi et al., 2002)

Gαq^Q203L.UAS, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, suppressible by fra^unspecified/fra[+]

(Ratnaparkhi et al., 2002)

Gαq^Q203L.UAS, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, suppressible by robo1^Y-F.UAS, Scer\GAL4^elav-C155

(Ratnaparkhi et al., 2002)

Enhancer of

Statement

Reference

Gαq^Q203L.UAS/Scer\GAL4^ftz.ng is an enhancer of abnormal neuroanatomy phenotype of robo1¹

(Ratnaparkhi et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Gαq^Q203L.UAS is a non-suppressor of abnormal neuroanatomy phenotype of fra³/fra⁴

(Ratnaparkhi et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gαq^Q203L.UAS, Scer\GAL4^elav-C155 has symmetrical commissure | embryonic stage phenotype, enhanceable by fra³/fra⁴

(Ratnaparkhi et al., 2002)

Gαq^Q203L.UAS, Scer\GAL4^elav-C155 has fascicle | embryonic stage phenotype, enhanceable by fra³/fra⁴

(Ratnaparkhi et al., 2002)

Gαq^Q203L.UAS, Scer\GAL4^ftz.ng has symmetrical commissure | embryonic stage phenotype, enhanceable by robo[+]/robo1¹

(Ratnaparkhi et al., 2002)

Gαq^Q203L.UAS, Scer\GAL4^ftz.ng has fascicle | embryonic stage phenotype, enhanceable by robo[+]/robo1¹

(Ratnaparkhi et al., 2002)

Gαq^Q203L.UAS, Scer\GAL4^elav-C155 has symmetrical commissure | embryonic stage phenotype, enhanceable by fra^unspecified/fra[+]

(Ratnaparkhi et al., 2002)

Gαq^Q203L.UAS, Scer\GAL4^elav-C155 has fascicle | embryonic stage phenotype, enhanceable by fra^unspecified/fra[+]

(Ratnaparkhi et al., 2002)

Suppressed by

Statement

Reference

Gαq^Q203L.UAS, Scer\GAL4^ninaE.PT has ommatidium | light conditional phenotype, suppressible | partially by PIP5K59B^{RNAi.UAS.L.cKa}/Scer\GAL4^GMR.PF

(Kumari et al., 2022)

Gαq^Q203L.UAS, Scer\GAL4^ninaE.PT has rhabdomere | light conditional | decreased number phenotype, suppressible | partially by PIP5K59B^{RNAi.UAS.L.cKa}/Scer\GAL4^GMR.PF

(Kumari et al., 2022)

Gαq^Q203L.UAS, Scer\GAL4^15J2 has dMP2 neuron phenotype, suppressible by robo1^UAS.Tag:MYC, Scer\GAL4^15J2

(Hiramoto and Hiromi, 2006)

Gαq^Q203L.UAS, Scer\GAL4^15J2 has dMP2 neuron phenotype, suppressible by fra³

(Hiramoto and Hiromi, 2006)

Gαq^Q203L.UAS, Scer\GAL4^15J2 has dMP2 neuron phenotype, suppressible by Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9

(Hiramoto and Hiromi, 2006)

Gαq^Q203L.UAS, Scer\GAL4^elav-C155 has symmetrical commissure | embryonic stage phenotype, suppressible by robo1^Y-F.UAS, Scer\GAL4^elav-C155

(Ratnaparkhi et al., 2002)

Gαq^Q203L.UAS, Scer\GAL4^elav-C155 has fascicle | embryonic stage phenotype, suppressible by robo1^Y-F.UAS, Scer\GAL4^elav-C155

(Ratnaparkhi et al., 2002)

Enhancer of

Statement

Reference

Gαq^Q203L.UAS/Scer\GAL4^ftz.ng is an enhancer of symmetrical commissure | embryonic stage phenotype of robo1¹

(Ratnaparkhi et al., 2002)

Gαq^Q203L.UAS/Scer\GAL4^ftz.ng is an enhancer of fascicle | embryonic stage phenotype of robo1¹

(Ratnaparkhi et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Gαq^Q203L.UAS is a non-suppressor of symmetrical commissure | embryonic stage phenotype of fra³/fra⁴

(Ratnaparkhi et al., 2002)

Scer\GAL4^elav-C155/Gαq^Q203L.UAS is a non-suppressor of fascicle | embryonic stage phenotype of fra³/fra⁴

(Ratnaparkhi et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Itp-r83A^GD1676, Unc-13-4B^GD7394, Rab3^KK108633 or Syt1^KK108653 (along with UAS-Dcr-2 expression) significantly suppresses larval lethality seen in Scer\GAL4^phm.PO>Gαq^{Q203L.Scer\UAS}.

(Yamanaka et al., 2015)

The arbor-like growth cone seen in dMP2 neurons expressing Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^15J2 is suppressed by fra³ and by Ts(1Lt;YSt)B118+Ts(1Rt;YLt)T9.

Co-expression of robo^{Scer\UAS.T:Hsap\MYC} suppresses the defects in the dMP2 neuron which are caused by expression of Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^15J2.

(Hiramoto and Hiromi, 2006)

In embryos expressing both Gα49B^{Q203L.Scer\UAS} and robo^Y-F.Scer\UAS under the regulation of Scer\GAL4^elav-C155, ectopic crossovers of the midline by axons. robo¹/+ heterozygous embryos, in which expression of Gα49B^{Q203L.Scer\UAS} is regulated under the control of Scer\GAL4^ftz.ng, exhibit a significant increase in the number of midline crossovers (of axons expressing Fas2), as compared to robo¹/+ mutant embryos and Gα49B^{Q203L.Scer\UAS};Scer\GAL4^ftz.ng embryos (316/623 abdominal segments exhibit midline crossing in the combined mutant compared to 12/245 in robo¹ and 86/427 in Gα49B^{Q203L.Scer\UAS};Scer\GAL4^ftz.ng). Removal of a single copy of the fra gene leads to a threefold reduction in the number of midline crossovers induced by Gα49B^{Q203L.Scer\UAS} under the control of Scer\GAL4^elav-C155 (from 48.10% to 15.3% of abdominal segments exhibit midline crossing). A further reduction is observed upon removal of both copies of the fra gene in Scer\GAL4^elav-C155/Gα49B^{Q203L.Scer\UAS};fra³/fra⁴, from 48.10% of abdominal segments exhibiting midline crossover in Scer\GAL4^elav-C155/Gα49B^{Q203L.Scer\UAS} embryos to 5.3% in Scer\GAL4^elav-C155/Gα49B^{Q203L.Scer\UAS};fra³/fra⁴ mutant embryos. Embryos of the genotype Scer\GAL4^elav-C155/Gα49B^{Q203L.Scer\UAS} and fra³/fra⁴ exhibit breaks in Con-positive commissural axons and longitudinal tracts, similar to Scer\GAL4^elav-C155/Gα49B^{Q203L.Scer\UAS}; fra³/fra⁴ embryos, indicating that Gα49B does not have an effect on the fra mutant phenotype.

(Ratnaparkhi et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

602862

y¹ w^*; P{UAS-Gαq.Q203L}F58c

30743

P{UAS-Gαq.Q203L}F58a, w^*/FM7i, P{ActGFP}JMR3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Gα49B^{Q203L.Scer\UAS}

Gαq^{Q203L.Scer\UAS}

Gαq^Q203L.UAS

Name Synonyms

Secondary FlyBase IDs

References (9)

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