FlyBase Allele Report: Dmel\Vrp1[f06715]

General Information

Symbol

Dmel\Vrp1^f06715

Species

D. melanogaster

Name

FlyBase ID

FBal0187335

Feature type

allele

Associated gene

Dmel\Vrp1

Associated Insertion(s)

PBac{WH}Vrp1^f06715

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{WH}Vrp1^f06715

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:22,109,217..22,109,238 [+]

(Hamp et al., 2016, Eriksson et al., 2010, Berger et al., 2008, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Eriksson et al., 2010)

Phenotype Manifest In

embryonic myoblast

(Kaipa et al., 2013)

embryonic somatic muscle cell

(Eriksson et al., 2010)

myoblast | embryonic stage

(Berger et al., 2008)

plasma membrane | embryonic stage

(Berger et al., 2008)

Detailed Description

Statement

Reference

Longitudinal muscle development is not significantly disturbed in Vrp1^f06715/Vrp1^D30 transheterozygote embryos.

(Rudolf et al., 2014)

Homozygous embryos show defects in myoblast fusion.

(Kaipa et al., 2013)

Vrp1^f06715 and Vrp1^f06715/Df(2R)ED3943 mutants display a severe embryonic somatic muscle phenotype with strong muscle fusion defects.

In the visceral mesoderm of Vrp1^f06715 embryos the founder cells and the fusion competent myoblasts appear to fuse despite the absence of Vrp1. The visceral mesoderm of Vrp1^f06715 mutants appears slightly disorganised at early stages, however the development of the gut proceeds, and Vrp1^f06715 mutants develop a gut structure with midgut constrictions at later stages.

At stage 12, in both Vrp1^f06715 and control embryos the longitudinal muscles surround the circular musculature, and at later stage, both in mutants and controls, the longitudinal muscles form a characteristic longitudinal pattern, indicating that longitudinal muscle development is not affected in Vrp1^f06715 mutants.

(Eriksson et al., 2010)

Some myoblast fusion takes place in Vrp1^f06715 mutants, but growing myotubes are still surrounded by numerous unfused myoblasts at the end of embryogenesis.

(Berger et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Vrp1^f06715 has embryonic myoblast phenotype, enhanceable by dock⁰⁴⁷²³

(Kaipa et al., 2013)

Vrp1^f06715 has embryonic myoblast phenotype, enhanceable by WASp³

(Kaipa et al., 2013)

Vrp1^f06715 has myoblast | embryonic stage phenotype, enhanceable by SCAR^Δ37

(Berger et al., 2008)

Vrp1^f06715 has plasma membrane | embryonic stage phenotype, enhanceable by SCAR^Δ37

(Berger et al., 2008)

NOT Suppressor of

Statement

Reference

Vrp1^f06715 is a non-suppressor of visceral mesoderm phenotype of Alk¹⁰

(Eriksson et al., 2010)

Vrp1^f06715 is a non-suppressor of embryonic myoblast phenotype of Alk¹⁰

(Eriksson et al., 2010)

Other

Statement

Reference

SCAR^Δ37, Vrp1^f06715 has longitudinal visceral muscle primordium phenotype

(Rudolf et al., 2014)

SCAR^Δ37, Vrp1^f06715 has embryonic/larval gut | embryonic stage phenotype

(Rudolf et al., 2014)

SCAR^Δ37, Vrp1^f06715 has embryonic/larval midgut longitudinal visceral muscle cell | embryonic stage phenotype

(Rudolf et al., 2014)

SCAR^Δ37, Vrp1^f06715/Vrp1[+] has embryonic/larval midgut longitudinal visceral muscle cell | embryonic stage phenotype

(Rudolf et al., 2014)

SCAR^Δ37/SCAR[+], Vrp1^f06715 has embryonic/larval midgut longitudinal visceral muscle cell | embryonic stage phenotype

(Rudolf et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

SCAR^Δ37;Vrp1^f06715 double homozygous mutant embryos show normal gut constriction but aberrant morphology. Longitudinal visceral muscle founder cells show aberrant migration and severe muscle fusion defects are observed: mostly mononucleated and binucleated muscle cells are seen at late embryonic stages. These defects are weaker in SCAR^Δ37/SCAR^Δ37;Vrp1^f06715/+ embryos (founder cell migration mostly normal though fusion defects are still observed) and not much improved in SCAR^Δ37/+;Vrp1^f06715/Vrp1^f06715 embryos (both founder cell migration and muscle fusion defects observed).

(Rudolf et al., 2014)

dock⁰⁴⁷²³ enhances the myoblast fusion defects seen in Vrp1^f06715 embryos.

WASp³ enhances the myoblast fusion defects seen in Vrp1^f06715 embryos.

(Kaipa et al., 2013)

Loss of Vrp1 through a Vrp1^f06715 mutant background does not affect fusion in Alk¹⁰ mutants, as mutant cells of the visceral mesoderm are able to both migrate and fuse with cells of the somatic mesoderm.

(Eriksson et al., 2010)

SCAR^Δ37 Vrp1^f06715 double mutants exhibit a dramatically increased myoblast-fusion defect compared to the single mutants.

(Berger et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Vrp1^f06715 is rescued by Scer\GAL4^sns.PS/Vrp1^{UAS.fl.Tag:MYC}

(Eriksson et al., 2010)

Vrp1^f06715 is rescued by Scer\GAL4^twi.PG/Vrp1^{UAS.fl.Tag:MYC}

(Eriksson et al., 2010)

Vrp1^f06715 is rescued by Scer\GAL4^sns.PS/Vrp1^{Δ2xWH2.UAS.Tag:MYC}

(Eriksson et al., 2010)

Vrp1^f06715 is rescued by Scer\GAL4^twi.PG/Vrp1^{Δ2xWH2.UAS.Tag:MYC}

(Eriksson et al., 2010)

Not rescued by

Vrp1^f06715 is not rescued by Scer\GAL4^sns.PS/Vrp1^{ΔProΔWBD.UAS.Tag:MYC}

(Eriksson et al., 2010)

Vrp1^f06715 is not rescued by Scer\GAL4^twi.PG/Vrp1^{ΔProΔWBD.UAS.Tag:MYC}

(Eriksson et al., 2010)

Vrp1^f06715 is not rescued by Scer\GAL4^sns.PS/Vrp1^{ΔWBD.UAS.Tag:MYC}

(Eriksson et al., 2010)

Vrp1^f06715 is not rescued by Scer\GAL4^twi.PG/Vrp1^{ΔWBD.UAS.Tag:MYC}

(Eriksson et al., 2010)

Comments

Expression of Vrp1^{Scer\UAS.fl.T:Hsap\MYC} in fusion-competent myoblasts under the control of Scer\GAL4^sns.PS fully rescues the muscle fusion defects found in Vrp1^f06715 mutants.

Expression of Vrp1^{Scer\UAS.fl.T:Hsap\MYC} in muscle cells under the control of Scer\GAL4^twi.PG fully rescues the muscle fusion defects found in Vrp1^f06715 mutants.

Expression of Vrp1^{Δ2xWH2.Scer\UAS.T:Hsap\MYC} in fusion-competent myoblasts under the control of Scer\GAL4^sns.PS fully rescues the muscle fusion defects found in Vrp1^f06715 mutants.

Expression of Vrp1^{Δ2xWH2.Scer\UAS.T:Hsap\MYC} in muscle cells under the control of Scer\GAL4^twi.PG fully rescues the muscle fusion defects found in Vrp1^f06715 mutants.

(Eriksson et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG13503^f06715

Vrp1^f06715

vrp1^f06715

(Hamp et al., 2016, Kaipa et al., 2013)

vrp^f06715

(Hamp et al., 2016)

wip^f06715

(Rudolf et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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