FlyBase Allele Report: Dmel\tok[UAS.cSa.Tag:HA]

General Information

Symbol

Dmel\tok^{UAS.cSa.Tag:HA}

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Serpe

FlyBase ID

FBal0190090

Feature type

allele

Associated gene

Dmel\tok

Associated Insertion(s)

Carried in Construct

P{UAS-tok.S}

Also Known As

UAS-tlr, UAS-tlr-HA

Key Links

Transgenic product class

wild_type

(Serpe et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Serpe et al., 2005)

Mutagen

in vitro construct

(Serpe et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-tok.S}

Cytology

Description

This construct is tagged at the C-terminus with Tag:HA.

(Serpe and O'Connor, 2006)

UASt sequences drive expression of tok.

(Serpe et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

tok

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^how-24B

(Meyer and Aberle, 2006)

Phenotype Manifest In

wing, with Scer\GAL4^how-24B

(Meyer and Aberle, 2006)

Detailed Description

Statement

Reference

Expression of tok^{Scer\UAS.cSa.T:Ivir\HA1} under the control of Scer\GAL4^G14 or Scer\GAL4^elav.PLu does not result in any motor axon guidance defects.

Expression of tok^{Scer\UAS.cSa.T:Ivir\HA1} under the control of Scer\GAL4^how-24B leads to a large central blister in the wing.

(Meyer and Aberle, 2006)

Expression of tok^{Scer\UAS.cSa.T:Ivir\HA1} under the control of Scer\GAL4^Cg.PA does not result in a detectable phenotype.

(Serpe and O'Connor, 2006)

Expression of two copies of tok^Scer\UAS.cSa under the control of Scer\GAL4^A9 does not affect posterior crossvein formation. Expression of tok^Scer\UAS.cSa under the control of Scer\GAL4^da.G32 largely restores posterior crossvein patterning in the adult wing.

(Serpe et al., 2005)

Expression of two copies of tok^Scer\UAS.cSa under the control of Scer\GAL4^A9 does not affect posterior crossvein formation.

(Serpe et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^A9/tok^{UAS.cSa.Tag:HA} is an enhancer of visible phenotype of Scer\GAL4^A9, sog^u.UAS

(Peluso et al., 2011)

Scer\GAL4^Cg.PA/tok^{UAS.cSa.Tag:HA} is an enhancer of some die during P-stage phenotype of Scer\GAL4^Cg.PA, daw^UAS.cSa

(Serpe and O'Connor, 2006)

Suppressor of

Statement

Reference

Scer\GAL4^en.PU/tok^{UAS.cSa.Tag:HA} is a suppressor of visible | adult stage phenotype of Paics^X1

(Schleede and Blair, 2015)

Scer\GAL4^A9/tok^{UAS.cSa.Tag:HA} is a suppressor of visible phenotype of Scer\GAL4^A9, sog^UAS.cYa, tsg^UAS.cYa

(Peluso et al., 2011)

NOT Suppressor of

Statement

Reference

tok^{UAS.cSa.Tag:HA}, Scer\GAL4^A9 is a non-suppressor of visible | adult stage phenotype of Gyc76C^GD48, Scer\GAL4^A9

(Schleede and Blair, 2015)

Other

Statement

Reference

Scer\GAL4^A9, sog^u.UAS, tok^{UAS.cSa.Tag:HA}, tsg^UAS.cYa has visible phenotype

(Peluso et al., 2011)

Scer\GAL4^A9, babo^c.UAS, daw^UAS.cSa, tok^{UAS.cSa.Tag:HA} has visible phenotype

(Jensen et al., 2009)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^A9/tok^{UAS.cSa.Tag:HA} is an enhancer of posterior crossvein phenotype of Scer\GAL4^A9, sog^u.UAS

(Peluso et al., 2011)

Scer\GAL4^A9/tok^{UAS.cSa.Tag:HA} is an enhancer of posterior crossvein phenotype of Scer\GAL4^A9, tld^UAS.cHa

(Serpe et al., 2005)

Suppressor of

Statement

Reference

Scer\GAL4^en.PU/tok^{UAS.cSa.Tag:HA} is a suppressor of posterior crossvein phenotype of Paics^X1

(Schleede and Blair, 2015)

Scer\GAL4^A9/tok^{UAS.cSa.Tag:HA} is a suppressor of wing blade phenotype of Scer\GAL4^A9, sog^UAS.cYa, tsg^UAS.cYa

(Peluso et al., 2011)

Scer\GAL4^A9/tok^{UAS.cSa.Tag:HA} is a suppressor of wing vein phenotype of Scer\GAL4^A9, sog^UAS.cYa, tsg^UAS.cYa

(Peluso et al., 2011, Serpe et al., 2005)

Scer\GAL4^A9/tok^{UAS.cSa.Tag:HA} is a suppressor of wing phenotype of Scer\GAL4^A9, sog^UAS.cYa, tsg^UAS.cYa

(Serpe et al., 2005)

NOT Suppressor of

Statement

Reference

tok^{UAS.cSa.Tag:HA}, Scer\GAL4^A9 is a non-suppressor of wing blade phenotype of Gyc76C^GD48, Scer\GAL4^A9

(Schleede and Blair, 2015)

tok^{UAS.cSa.Tag:HA}, Scer\GAL4^A9 is a non-suppressor of posterior crossvein phenotype of Gyc76C^GD48, Scer\GAL4^A9

(Schleede and Blair, 2015)

Other

Statement

Reference

Scer\GAL4^A9, sog^u.UAS, tok^{UAS.cSa.Tag:HA}, tsg^UAS.cYa has wing vein phenotype

(Peluso et al., 2011)

Scer\GAL4^A9, sog^u.UAS, tok^{UAS.cSa.Tag:HA}, tsg^UAS.cYa has wing blade phenotype

(Peluso et al., 2011)

Scer\GAL4^A9, babo^c.UAS, daw^UAS.cSa, tok^{UAS.cSa.Tag:HA} has wing vein phenotype

(Jensen et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The partial loss of the wing posterior crossvein characteristic for Paics^X1 mutant adults is rescued by expression of tok^{Scer\UAS.cSa.T:Ivir\HA1} under the control of Scer\GAL4^en.PU in the mutant background.

The disruption of the posterior crossvein characteristic for flies expressing Gyc76C^GD48 under the control of Scer\GAL4^A9 is not improved by co-expression of any of tok^{Scer\UAS.cSa.T:Ivir\HA1}.

(Schleede and Blair, 2015)

The loss of the posterior crossvein due to sog^u.Scer\UAS-overexpression is exacerbated when tok^{Scer\UAS.cSa.T:Ivir\HA1} is also expressed under the control of Scer\GAL4^A9.

Co-overexpression of tok^{Scer\UAS.cSa.T:Ivir\HA1} with sog^Scer\UAS.cYa and tsg^Scer\UAS.cYa under the control of Scer\GAL4^A9 is able to reverse the sog^Scer\UAS.cYa-tsg^Scer\UAS.cYa small-wing phenotype and restore normal patterning.

Co-overexpression of tok^{Scer\UAS.cSa.T:Ivir\HA1} with sog^u.Scer\UAS and tsg^Scer\UAS.cYa under the control of Scer\GAL4^A9 results in a small-wing phenotype and abnormal wing vein patterning.

(Peluso et al., 2011)

Expression of daw^Scer\UAS.cSa and tok^{Scer\UAS.cSa.T:Ivir\HA1} in the wing under the control of Scer\GAL4^A9 does not result in any wing vein-patterning defects.

Expression of babo^c.Scer\UAS, daw^Scer\UAS.cSa and tok^{Scer\UAS.cSa.T:Ivir\HA1} in the wing under the control of Scer\GAL4^A9 results in a severe wing vein-patterning defects.

(Jensen et al., 2009)

Coexpression of tok^{Scer\UAS.cSa.T:Ivir\HA1} and daw^Scer\UAS.cSa, under the control of Scer\GAL4^Cg.PA results in over 50% pupal lethality, as opposed to under 10% in Scer\GAL4^Cg.PA>daw^Scer\UAS.cSa animals and no lethality in Scer\GAL4^Cg.PA>tok^{Scer\UAS.cSa.T:Ivir\HA1} animals.

(Serpe and O'Connor, 2006)

Coexpression of sog^Scer\UAS.cYa and tsg^Scer\UAS.cYa in the wing disc, under the control of Scer\GAL4^A9, produces a small wing with altered patterns of venation. Coexpression of tok^Scer\UAS.cSa with these two transgenes is able to reverse the small wing phenotype and restore normal patterning. Co-overexpression of tld^Scer\UAS.cHa and tok^Scer\UAS.cSa, under the control of Scer\GAL4^A9, leads to an increased penetrance and severity of the posterior crossvein phenotype compared to expression of tld^Scer\UAS.cHa alone.

(Serpe et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

tok^{UAS.cSa.Tag:HA}/Scer\GAL4^hs.PB rescues tok^K788/tok^D427

(Meyer and Aberle, 2006)

Scer\GAL4^G14/tok^{UAS.cSa.Tag:HA} rescues tok^K788/tok^D427

(Meyer and Aberle, 2006)

tok^{UAS.cSa.Tag:HA}/Scer\GAL4^elav.PLu rescues tok^K788/tok^D427

(Meyer and Aberle, 2006)

Scer\GAL4^srp/tok^{UAS.cSa.Tag:HA} rescues tok^K788/tok^D427

(Meyer and Aberle, 2006)

tok^{UAS.cSa.Tag:HA}/Scer\GAL4^ppl.PP rescues tok^K788/tok^D427

(Meyer and Aberle, 2006)

Scer\GAL4^elav-C155/tok^{UAS.cSa.Tag:HA} rescues tok^Δ2-41/tok^E1

(Serpe and O'Connor, 2006)

Scer\GAL4^12M/tok^{UAS.cSa.Tag:HA} rescues tok^Δ2-41/tok^E1

(Serpe and O'Connor, 2006)

Scer\GAL4^how-24B/tok^{UAS.cSa.Tag:HA} rescues tok^Δ2-41/tok^E1

(Serpe and O'Connor, 2006)

Scer\GAL4^G14/tok^{UAS.cSa.Tag:HA} rescues tok^Δ2-41/tok^E1

(Serpe and O'Connor, 2006)

Scer\GAL4^Cg.PA/tok^{UAS.cSa.Tag:HA} rescues tok^Δ2-41/tok^E1

(Serpe and O'Connor, 2006)

Scer\GAL4^phtm.PO/tok^{UAS.cSa.Tag:HA} rescues tok^Δ2-41/tok^E1

(Serpe and O'Connor, 2006)

Scer\GAL4^da.G32/tok^{UAS.cSa.Tag:HA} rescues tok^Δ2-41/tok^E1

(Serpe and O'Connor, 2006)

Partially rescues

tok^{UAS.cSa.Tag:HA}/Scer\GAL4^sca-109-68 partially rescues tok^Δ2-41/tok^E1

(Serpe and O'Connor, 2006)

Scer\GAL4^Mhc.PW/tok^{UAS.cSa.Tag:HA} partially rescues tok^Δ2-41/tok^E1

(Serpe and O'Connor, 2006)

Scer\GAL4^da.G32/tok^{UAS.cSa.Tag:HA} partially rescues tok^Δ2-41/tok^E1

(Serpe et al., 2005)

Fails to rescue

tok^{UAS.cSa.Tag:HA}/Scer\GAL4^ChAT.7.4 fails to rescue tok^K788/tok^D427

(Meyer and Aberle, 2006)

Comments

Expression of tok^{Scer\UAS.cSa.T:Ivir\HA1} under the control of Scer\GAL4^hs.PB using heat shock during mid-embryogenesis completely rescues the innervation defects on all muscles in tok^K788/tok^D427 animals.

Expression of tok^{Scer\UAS.cSa.T:Ivir\HA1} under the control of Scer\GAL4^G14, Scer\GAL4^elav.PLu, Scer\GAL4^ppl.PP or Scer\GAL4^srp completely rescues the muscle innervation defects in tok^K788/tok^D427 animals.

Expression of tok^{Scer\UAS.cSa.T:Ivir\HA1} under the control of Scer\GAL4^Cha.7.4 does not rescue the innervation defects on muscles that are seen in tok^K788/tok^D427 animals.

(Meyer and Aberle, 2006)

Expression of tok^Scer\UAS.cSa under the control of Scer\GAL4^da.G32 largely restores posterior crossvein patterning in the adult wing of tok^Δ2-41/tok^E1 mutants.

(Serpe et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

tok^{Scer\UAS.cSa.T:Ivir\HA1}

tok^Scer\UAS.cSa

tok^{UAS.cSa.Tag:HA}

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Serpe

(Serpe et al., 2005)

Secondary FlyBase IDs

References (6)

Report Sections

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