FlyBase Allele Report: Dmel\Dronc[I24]

General Information

Symbol

Dmel\Dronc^I24

Species

D. melanogaster

Name

FlyBase ID

FBal0190335

Feature type

allele

Associated gene

Dmel\Dronc

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Choi et al., 2006)

loss of function allele

(Arama et al., 2006)

Mutagen

ethyl methanesulfonate

(Arama et al., 2006, Xu et al., 2005)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?28term.

(Arama et al., 2006)

Amino acid replacement: W28term.

(Xu et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:9,968,970..9,968,970 [+]

Nucleotide change:

G9968970A

Amino acid change:

W28term | Dronc-PA

Reported amino acid change:

W28term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Xu et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death

(Lee et al., 2011, Baum et al., 2007)

abnormal cell death (with Dronc⁵¹)

(Lee et al., 2011, Choi et al., 2006)

decreased cell death (with Dronc^I29)

(Yacobi-Sharon et al., 2013)

decreased cell death (with Dronc^L32)

(Yacobi-Sharon et al., 2013)

decreased cell death | rescuable maternal effect

(Xu et al., 2005)

female sterile

(Arama et al., 2006)

increased cell death

(Liu et al., 2013)

increased cell number | somatic clone

(Verghese et al., 2012)

increased occurrence of cell division | larval stage | somatic clone

(Verghese et al., 2012)

lethal | embryonic stage | recessive | rescuable maternal effect

(Xu et al., 2005)

lethal | recessive

(Xu et al., 2005)

lethal - all die before end of pupal stage | partially

(Arama et al., 2006)

male sterile

(Arama et al., 2006)

some die during pupal stage | partially

(Arama et al., 2006)

visible

(Baum et al., 2007)

visible (with Dronc^I29)

(Krieser et al., 2007)

visible | male (with Dronc^I29)

(Krieser et al., 2007)

Phenotype Manifest In

arista

(Baum et al., 2007)

cystic bulge

(Arama et al., 2006)

dendrite

(Schoenmann et al., 2010)

egg chamber

(Baum et al., 2007)

embryonic head | rescuable maternal effect

(Xu et al., 2005)

eye | somatic clone

(Verghese et al., 2012)

eye-antennal disc | somatic clone

(Verghese et al., 2012)

eye disc | somatic clone

(Xu et al., 2005)

eye disc posterior to the morphogenetic furrow | somatic clone

(Verghese et al., 2012)

male genitalia (with Dronc^I29)

(Krieser et al., 2007)

male germline cell (with Dronc^I29)

(Yacobi-Sharon et al., 2013)

male germline cell (with Dronc^L32)

(Yacobi-Sharon et al., 2013)

neuron

(Lee et al., 2011)

neuron (with Dronc⁵¹)

(Lee et al., 2011)

neuron & ventral nerve cord | ectopic (with Dronc⁵¹)

(Choi et al., 2006)

pigment cell | somatic clone

(Verghese et al., 2012)

spermatid

(Arama et al., 2006)

spermatogonial cyst (with Dronc^I29)

(Yacobi-Sharon et al., 2013)

spermatogonial cyst (with Dronc^L32)

(Yacobi-Sharon et al., 2013)

waste bag

(Arama et al., 2006)

wing

(Xu et al., 2005)

wing (with Dronc^I29)

(Krieser et al., 2007)

Detailed Description

Statement

Reference

Nc^I24 mutants exhibit an increase in autophagy.

(Liu et al., 2013)

Nc^I24/Nc^I29 mutant males exhibit ~40% less spermatogonial cyst death compared to controls.

Nc^I24/Nc^L32 mutant males exhibit ~40% less spermatogonial cyst death compared to controls.

(Yacobi-Sharon et al., 2013)

Nc^I24 mutant eye clones are significantly larger than their wild-type twin spots.

Nc^I24 mutant eye clones induce ectopic cell proliferation posterior to the second mitotic wave in the eye-antennal disc.

Nc^I24 mutant eye clones exhibit extra ommatidial pigment cells in the pupal retina.

(Verghese et al., 2012)

Nc^I24 homozygotes and Nc^I24/Nc⁵¹ transheterozygotes exhibit a delay in vCrz neuron apoptosis. At 7 hours after puparium formation 75% vCrz neurons are still present although these all die by 48 hours after puparium formation.

(Lee et al., 2011)

Nc^I24 mutant dendrites exhibit an intact morphlogy before puparium formation. Unlike in wild-type, at 7.5 hours after puparium formation Nc^I24 mutant dendrites continue to possess an overall regular morphology and are mostly attached to the parental neurons. These branches persist even at 18 hours after puparium formation, albeit with less homogenous morphology.

(Schoenmann et al., 2010)

Homozygous adult survivors have approximately 6-8 extra branches on the posterior of the arista compared to wild type. Approximately 10% of stage 14 egg chambers contain persisting nurse cell nuclei.

(Baum et al., 2007)

Salivary glands are degraded by 24 hours after puparium formation (as occurs in wild-type controls) in Nc^I24/Nc^I29 mutants that develop normally.

(Berry and Baehrecke, 2007)

100% of Nc^I29/Nc^I24 male escapers have a rotated genitalia defect. 100% of Nc^I29/Nc^I24 adult escapers have a ballooned wing phenotype.

(Krieser et al., 2007)

Nc^I24 mutants display severe defects during the spermatid individualization process. The cystic bulges are frequently reduced in size or appear flat due to a failure in the appropriate collection of the cytoplasm of the spermatids . The retained cytoplasm is clearly visualized as a trail along the entire length of what was supposed to have been the post-individualized portion of the spermatids. Frequently a large portion of the spermatid cytoplasm is retained in a 'mini' cystic bulge structure, which often contains part of the individualization complex. Waste bags are also reduced in size.

(Arama et al., 2006)

Programmed cell death in Crz-expressing neurons in the ventral nerve cord is significantly delayed in Nc⁵¹/Nc^I24 mutants. Approximately 12 neurons still survive at 7 hours after pupal formation, while the number is reduced to approximately 8 at 16 hours after pupal formation, and none at 48 hours after pupal formation.

(Choi et al., 2006)

Homozygous escapers have wings that are less transparent than normal and are curved. Homozygous embryos derived from females carrying homozygous germline clones have a head defect. These embryos show a substantial reduction in the number of apoptotic cells compared to wild type. Homozygous clones in the pupal eye disc have on average three additional interommatidial cells.

(Xu et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Dronc^I24 has abnormal cell death phenotype, enhanceable by Strica⁴

(Lee et al., 2011)

Dronc^I24/Dronc⁵¹ has abnormal cell death phenotype, enhanceable by Strica⁴

(Lee et al., 2011)

NOT Enhanced by

Statement

Reference

Dronc^I24, Strica⁴ has abnormal cell death phenotype, non-enhanceable by Dredd^B118

(Lee et al., 2011)

Suppressed by

Statement

Reference

Dronc^I24/Dronc⁵¹ has abnormal cell death phenotype, suppressible by Dredd^L23

(Lee et al., 2011)

NOT suppressed by

Statement

Reference

Dronc^I24, Strica⁴ has abnormal cell death phenotype, non-suppressible by Dredd^B118

(Lee et al., 2011)

Enhancer of

Statement

Reference

Dronc^I24 is an enhancer of abnormal cell death phenotype of Strica⁴

(Lee et al., 2011)

Suppressor of

Statement

Reference

Dronc^I24/Dronc[+] is a suppressor of visible phenotype of Scer\GAL4^GMR.PU, Srrm1^GE25979

(Fan et al., 2014)

Dronc^I24/Nc[+] is a suppressor | partially of visible phenotype of HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

Dronc^I24/Nc[+] is a suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PF, elfless^UAS.cCa

(Caldwell et al., 2008)

Dronc^I24/Nc[+] is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, elfless^UAS.cCa

(Caldwell et al., 2008)

Dronc^I24/Dronc^I24 is a suppressor of visible | somatic clone phenotype of hid^GMR.PG

(Xu et al., 2005)

Dronc^I24/Dronc^I24 is a suppressor of visible | somatic clone phenotype of rpr^GMR.PW

(Xu et al., 2005)

NOT Suppressor of

Statement

Reference

Dronc^I24 is a non-suppressor of FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype of Df(1)su(s)R194, RpL36^+t4

(Kale et al., 2015)

Dronc^I24/Nc[+] is a non-suppressor of visible | somatic clone phenotype of hid^GMR.PG

(Xu et al., 2005)

Other

Statement

Reference

Dronc^I24, Strica⁴ has female fertile | germline clone phenotype

(Baum et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Dronc^I24 has neuron phenotype, enhanceable by Strica⁴

(Lee et al., 2011)

NOT Enhanced by

Statement

Reference

Dronc^I24, Strica⁴ has neuron phenotype, non-enhanceable by Dredd^B118

(Lee et al., 2011)

Suppressed by

Statement

Reference

Dronc^I24/Dronc⁵¹ has neuron phenotype, suppressible by Dredd^L23

(Lee et al., 2011)

NOT suppressed by

Statement

Reference

Dronc^I24, Strica⁴ has neuron phenotype, non-suppressible by Dredd^B118

(Lee et al., 2011)

Enhancer of

Statement

Reference

Dronc^I24 is an enhancer of neuron phenotype of Strica⁴

(Lee et al., 2011)

Dronc^I24/Dronc^I29 is an enhancer of embryonic/larval salivary gland phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^fkh.PH

(Berry and Baehrecke, 2007)

Suppressor of

Statement

Reference

Dronc^I24/Dronc[+] is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, Srrm1^GE25979

(Fan et al., 2014)

Dronc^I24/Nc[+] is a suppressor | partially of wing phenotype of HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

Dronc^I24/Nc[+] is a suppressor of pigment cell phenotype of Scer\GAL4^GMR.PF, elfless^UAS.cCa

(Caldwell et al., 2008)

Dronc^I24/Nc[+] is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, elfless^UAS.cCa

(Caldwell et al., 2008)

Dronc^I24/Dronc^I24 is a suppressor of eye | somatic clone phenotype of hid^GMR.PG

(Xu et al., 2005)

Dronc^I24/Dronc^I24 is a suppressor of eye | somatic clone phenotype of rpr^GMR.PW

(Xu et al., 2005)

Dronc^I24/Dronc^L32 is a suppressor | partially of ovariole phenotype of Diap1^6B/Diap1⁸

(Xu et al., 2005)

NOT Suppressor of

Statement

Reference

Dronc^I24/Nc[+] is a non-suppressor of eye | somatic clone phenotype of hid^GMR.PG

(Xu et al., 2005)

Other

Statement

Reference

Dronc^I24, Strica⁴ has egg chamber | germline clone phenotype

(Baum et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Dronc^I24 slightly suppresses the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

(Fan et al., 2014)

No viable vCrz neurons are detected at 7 hours after puparium formation in Dredd^L23; Nc^I24/Nc⁵¹ double mutants.

Nearly all vCrz neurons are present at 7 hours after puparium formation in dream⁴; Nc^I24 double mutants. Such an abnormal programmed cell death phenotype is significantly more severe than in either single mutant. No surviving vCrz neurons are found at 7 hours after puparium formation in dream⁴; Nc^I24/+ mutants, suggesting that heterozygosity of Nc is sufficient to compensate for the lack of dream function.

Triple mutants of Dredd^B118; dream⁴; Nc^I24 display results comparable to dream⁴; Nc^I24 double mutants, i.e. all vCrz neurons are still present having not undergone apoptosis by 7 hours after puparium formation.

(Lee et al., 2011)

Dcp-1^Prev1 ; Nc^I24/Nc^I29 Ice^Δ1 triple mutant larvae undergo cell death with morphology similar to the midgut of wild-type animals when analysed from -4 to -1 hours relative to puparium formation.

dream⁴ ; Nc^I24/Nc^I29 Ice^Δ1 triple mutant larvae show high levels of TUNEL staining at -4 to -1 hours relative to puparium formation, suggesting that they are undergoing cell death.

(Denton et al., 2009)

Females containing dream⁴ ; Nc^I24 double homozygous germline clones are fertile, but their ovaries contain 31% of mid-stage egg chambers with missing follicle cells and large surviving nurse cells. There is also an increase in mid-stage egg chambers containing condensed nurse cell nuclei but lacking follicle cells. 16% of stage 14 egg chambers contain persisting nurse cell nuclei.

(Baum et al., 2007)

The persistence of salivary gland tissue that is seen at 24 hours hours after puparium formation when Pi3K92E^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^fkh.PH is significantly enhanced if the animals are also carrying Nc^I24/Nc^I29.

(Berry and Baehrecke, 2007)

Xenogenetic Interactions

Statement

Reference

The adult wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1 are partially suppressed by the presence of Nc^I24/+.

(Marchal et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99694

w^*; Dronc^I24 P{neoFRT}80B/TM3, Sb¹

Notes on Origin

Discoverer

Selected as: a recessive suppressor of the W^GMR.PG eye ablation phenotype.

(Xu et al., 2005)

Selected as: a mutant that recessively suppresses the eye ablation phenotype caused by eye-specific overexpression of W.

(Arama et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dronc^I24

(Erclik et al., 2017, Bertet et al., 2014, Liu et al., 2013, Caldwell et al., 2008)

Nc^I24

dronc¹²⁴

(Krieser et al., 2007)

dronc^I24

(Amcheslavsky et al., 2018, Hara et al., 2018, Kamber Kaya et al., 2017, Kale et al., 2015, Fan et al., 2014, Lee et al., 2013, Yacobi-Sharon et al., 2013, Florentin and Arama, 2012, Marchal et al., 2012, Verghese et al., 2012, Lee et al., 2011, Schoenmann et al., 2010, Denton et al., 2009, Baum et al., 2007, Berry and Baehrecke, 2007, Arama et al., 2006, Choi et al., 2006, Xu et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (23)

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