FB2024_03 , released June 25, 2024
Allele: Dmel\EcRA-ΔC655.F645A.UAS
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General Information
Symbol
Dmel\EcRA-ΔC655.F645A.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0190469
Feature type
allele
Associated gene
Dmel\EcR
Associated Insertion(s)
Carried in Construct
P{UAS-EcR.A.F645A}
Also Known As
Ecr-A-F645A
Key Links
Transgenic product class
inframe_deletion
(Brown et al., 2006)
missense_variant
(Brown et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Brown et al., 2006)
missense_variant
(Brown et al., 2006)
Mutagen
in vitro construct
(Brown et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-EcR.A.F645A}
Cytology
Description

UASt regulatory sequences drive expression of a mutated EcR A isoform (carries the amino acid replacement F645A) that is truncated after residue 655.

(Brown et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
EcR
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression via Scer\GAL4ey-OK107 completely prevents formation of the late born alpha/beta neurons in mushroom bodies.

      Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN prolongs the lifetime and prevents shrinkage of pupal neuroblasts (NBs). These longer lived NBs continue dividing for a longer time and generate more neuronal progeny than wild type.

      Expression via Scer\GAL4insc-Mz1407 reduces the difference (increase) in oxygen consumption rates seen in wild type larval vs pupal brains. NADH levels are decreased, but overall energy/ATP levels are unchanged. Lactate levels are reduced in larvae and increased in pupae, effectively reducing the difference between the two stages, whereas lactate levels strongly decrease from larval to pupal stages in wild type.

      (Homem et al., 2014)

      Muscle expression of EcRA-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4Mhc.PW produces a delay in synapse elimination due to post-synaptic disruption of EcR signaling.

      Expression of EcRA-ΔC655.F645A.Scer\UAS in presynaptic neurons (under the control of Scer\GAL4OK6 does not delay synapse elimination.

      Expression of EcRA-ΔC655.F645A.Scer\UAS postsynaptically (under the control of Scer\GAL4OK6) in 9 hour APF pupae does not induce presynaptic dismantling such as synaptic vesicle aggregation, decreased bouton number, or enlarged bouton size, while post-synaptic dismantling occurs normally.

      (Liu et al., 2010)

      Specific overexpression of EcRA-ΔC655.F645A.Scer\UAS in the eye, under the control of Scer\GAL4GMR.PF, disrupts differentiation of the ommatidia and eye patterning. This results in a decrease in pigment cells, collapse of the eye, and characteristic scarring.

      Inhibition of EcR function by the expression of EcRA-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4e22c has no effect on wing size.

      (Cakouros et al., 2008)

      BrdU incorporation and the number of mitotic cells is reduced in clones in the wing disc expressing EcRA-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4Act5C.PP compared to control clones.

      (Mitchell et al., 2008)

      Thoracic ventral neurosecretory cells that express EcRA-ΔC655.F645A.Scer\UAS under the control of Scer\GAL4Fmrf.PS are of normal size and larval morphology at the start of metamorphosis. However, these mutants show defective neuronal remodelling during metamorphosis. The axons show much lower levels of pruning compared to wild-type cells and show no filopodia during the pruning phase.

      (Brown et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Knock-down of LKR through LKRIR.Scer\UAS partially restores the pigmentation defect found in EcRA-ΔC655.F645A.Scer\UAS (Scer\GAL4GMR.PF) mutants and display a striking decrease of scarring compared to expression of EcRA-ΔC655.F645A.Scer\UAS (Scer\GAL4GMR.PF) alone.

      Expression of EcRA-ΔC655.F645A.Scer\UAS restores the small wing phenotype caused by LKRIR.Scer\UAS.

      (Cakouros et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of EcRA-ΔC655.F645A.Scer\UAS slightly enhances the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1Scer\UAS.cSa is expressed under the control of Scer\GAL4Hml.Δ.

      (Sinenko et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      EcRA-ΔC655.F645A.Scer\UAS
      EcRA-ΔC655.F645A.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (13)