FlyBase Allele Report: Dmel\LamC[UAS.cSa]

General Information

Symbol

Dmel\LamC^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Schulze

FlyBase ID

FBal0190837

Feature type

allele

Associated gene

Dmel\LamC

Associated Insertion(s)

Carried in Construct

P{UAS-LamC.S}

Also Known As

UAS-LamC, UAS-lamin C, WT LamC

Key Links

Transgenic product class

cDNA

(Schulze et al., 2005)

wild_type

(Schulze et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Schulze et al., 2005)

wild_type

(Schulze et al., 2005)

Mutagen

in vitro construct

(Schulze et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-LamC.S}

Cytology

Description

UASt regulatory sequences drives expression of a wild-type LamC cDNA.

(Schulze et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

LamC

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Scer\GAL4^Act5C.PI

(Dialynas et al., 2010)

viable, with Scer\GAL4^C57

(Walker et al., 2023, Shaw et al., 2022, Dialynas et al., 2012, Dialynas et al., 2010)

viable, with Scer\GAL4^CCAP.PP

(Dialynas et al., 2010)

viable, with Scer\GAL4^dimm-929

(Dialynas et al., 2010)

viable, with Scer\GAL4^ey.PU

(Dialynas et al., 2012)

viable, with Scer\GAL4^futsch-C380

(Dialynas et al., 2010)

viable, with Scer\GAL4^how-24B

(Dialynas et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Dialynas et al., 2012, Dialynas et al., 2010)

viable, with Scer\GAL4^Mhc.PU

(Dialynas et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of LamC^UAS.cSa under the control of Scer\GAL4^fln.IFM, Scer\GAL4^Act88F.PB or Scer\GAL4^EDTP-DJ694 does not affect flight ability; this occurs in an otherwise wild-type background or in a LamC^EX296 heterozygous background.

Expressing LamC^UAS.cSa under the control of Scer\GAL4^Act88F.PB does not affect the sarcomere structure in pupal indirect flight muscles, with disrupted Z-discs and M-lines.

Expressing LamC^UAS.cSa under the control of Scer\GAL4^fln.IFM does not affect wing posture and the indirect flight muscles do not seem to show major morphological/ultrastructural defects.

(Chandran et al., 2019)

Expressing LamC^UAS.cSa under the control of Scer\GAL4^HCH.Hand does not affect flight ability and does not seem to cause morphological and physiological defects in the adult heart. 3-week-old cardiomyocytes do not show nuclear envelope, Z-disc or myofibril organization defects.

(Bhide et al., 2018)

Expression of LamC^Scer\UAS.cSa under the control of Scer\GAL4^C57 causes no obvious phenotypes and does not affect viability.

(Dialynas et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Atg1^K38Q.UAS, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has short lived phenotype, enhanceable by cnc^RNAi.UAS.cUa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

NOT Enhanced by

Statement

Reference

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has short lived phenotype, non-enhanceable by Atg1^K38Q.UAS, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has short lived phenotype, non-enhanceable by Atg1^UAS.cSa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Atg1^UAS.cSa, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has short lived phenotype, non-enhanceable by cnc^RNAi.UAS.cUa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Other

Statement

Reference

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has short lived phenotype

(Bhide et al., 2018)

GstD4^GD16151, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has short lived phenotype

(Bhide et al., 2018)

GstD9^KK113031, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has short lived phenotype

(Bhide et al., 2018)

Atg1^UAS.cSa, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has short lived phenotype

(Bhide et al., 2018)

Atg1^K38Q.UAS, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has short lived phenotype

(Bhide et al., 2018)

Keap1^KK102175, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has short lived phenotype

(Bhide et al., 2018)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has adult heart phenotype, non-enhanceable by Atg1^UAS.cSa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has nucleus | adult stage phenotype, non-enhanceable by Atg1^UAS.cSa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has adult heart phenotype, non-enhanceable by Atg1^K38Q.UAS, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has nucleus | adult stage phenotype, non-enhanceable by Atg1^K38Q.UAS, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has nuclear envelope | adult stage phenotype, non-enhanceable by Atg1^K38Q.UAS, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has myofibril | adult stage phenotype, non-enhanceable by Atg1^K38Q.UAS, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Atg1^K38Q.UAS, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has adult heart phenotype, non-enhanceable by cnc^RNAi.UAS.cUa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Atg1^K38Q.UAS, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nucleus | adult stage phenotype, non-enhanceable by cnc^RNAi.UAS.cUa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Atg1^K38Q.UAS, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nuclear envelope | adult stage phenotype, non-enhanceable by cnc^RNAi.UAS.cUa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Atg1^K38Q.UAS, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has myofibril | adult stage phenotype, non-enhanceable by cnc^RNAi.UAS.cUa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has nuclear envelope | adult stage phenotype, non-enhanceable by Atg1^UAS.cSa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has myofibril | adult stage phenotype, non-enhanceable by Atg1^UAS.cSa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Atg1^UAS.cSa, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has adult heart phenotype, non-enhanceable by cnc^RNAi.UAS.cUa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Atg1^UAS.cSa, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nucleus | adult stage phenotype, non-enhanceable by cnc^RNAi.UAS.cUa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Atg1^UAS.cSa, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nuclear envelope | adult stage phenotype, non-enhanceable by cnc^RNAi.UAS.cUa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Atg1^UAS.cSa, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has myofibril | adult stage phenotype, non-enhanceable by cnc^RNAi.UAS.cUa, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Suppressor of

Statement

Reference

LamC^UAS.cSa/Scer\GAL4^NP1624 is a suppressor | partially of male germline cell phenotype of Lam^GD1478

(Chen et al., 2013)

Other

Statement

Reference

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has adult heart phenotype

(Bhide et al., 2018)

GstD4^GD16151, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has adult heart phenotype

(Bhide et al., 2018)

GstD4^GD16151, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has myofibril | adult stage phenotype

(Bhide et al., 2018)

GstD4^GD16151, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nucleus | adult stage phenotype

(Bhide et al., 2018)

GstD4^GD16151, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nuclear envelope | adult stage phenotype

(Bhide et al., 2018)

GstD9^KK113031, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has adult heart phenotype

(Bhide et al., 2018)

GstD9^KK113031, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has myofibril | adult stage phenotype

(Bhide et al., 2018)

GstD9^KK113031, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nucleus | adult stage phenotype

(Bhide et al., 2018)

GstD9^KK113031, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nuclear envelope | adult stage phenotype

(Bhide et al., 2018)

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has myofibril | adult stage phenotype

(Bhide et al., 2018)

Atg1^UAS.cSa, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has adult heart phenotype

(Bhide et al., 2018)

Atg1^UAS.cSa, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has myofibril | adult stage phenotype

(Bhide et al., 2018)

Atg1^UAS.cSa, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nucleus | adult stage phenotype

(Bhide et al., 2018)

Atg1^UAS.cSa, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nuclear envelope | adult stage phenotype

(Bhide et al., 2018)

Atg1^K38Q.UAS, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has adult heart phenotype

(Bhide et al., 2018)

Atg1^K38Q.UAS, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has myofibril | adult stage phenotype

(Bhide et al., 2018)

Atg1^K38Q.UAS, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nucleus | adult stage phenotype

(Bhide et al., 2018)

Atg1^K38Q.UAS, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nuclear envelope | adult stage phenotype

(Bhide et al., 2018)

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has nucleus | adult stage phenotype

(Bhide et al., 2018)

LamC^UAS.cSa, Scer\GAL4^HCH.Hand, cnc^RNAi.UAS.cUa has nuclear envelope | adult stage phenotype

(Bhide et al., 2018)

Keap1^KK102175, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has adult heart phenotype

(Bhide et al., 2018)

Keap1^KK102175, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has myofibril | adult stage phenotype

(Bhide et al., 2018)

Keap1^KK102175, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nucleus | adult stage phenotype

(Bhide et al., 2018)

Keap1^KK102175, LamC^UAS.cSa, Scer\GAL4^HCH.Hand has nuclear envelope | adult stage phenotype

(Bhide et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of LamC^UAS.cSa with either AMPKα^UAS.cMa, AMPKα^K56R.UAS, AMPKα^T184D.UAS, AMPKα^HMC04979, srl^UAS.cRa, srl^KK100201, foxo^UASp.GFP, foxo^KK108485, Thor^UAS.cMa, Thor^KK108272, Thor^LL.UAS, S6k^TE.UAS or S6k^KQ.UAS, under the control of Scer\GAL4^fln.IFM, does not lead to any obvious indirect flight muscle, wing posture or flight defects.

(Chandran et al., 2019)

Co-expressing LamC^UAS.cSa with either cnc^{dsRNA.UAS.cUa}, Keap1^KK102175, GstD4^GD16151, GstD9^KK113031, Atg1^UAS.cSa or Atg1^K38Q.UAS under the control of Scer\GAL4^HCH.Hand causes mild cardiac physiology defects, with cardiomyocytes myofibril organization defects.

(Bhide et al., 2018)

Co-expression of LamC^Scer\UAS.cSa partially rescues the germline stem cell differentiation defect seen in males expressing Lam^GD1478 under the control of Scer\GAL4^tj-NP1624 : 58.9% of testes show strong rescue (although they are shorter than normal), containing germ cells at all stages of differentiation. The remaining 41.1% of testes show weak rescue, showing less severe accumulation of early germ cells but still appearing abnormal.

(Chen et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

LamC^UAS.cSa/Scer\GAL4^Act5C.PI partially rescues LamC^LC58

(Uchino et al., 2013)

Scer\GAL4^sr-md710/LamC^UAS.cSa partially rescues LamC^LC58

(Uchino et al., 2013)

LamC^UAS.cSa/Scer\GAL4^how-24B partially rescues LamC^LC58

(Uchino et al., 2013)

Scer\GAL4^NP3076/LamC^UAS.cSa partially rescues LamC^LC58

(Uchino et al., 2013)

Comments

Expression of LamC^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PI rescues head eversion in 13% of LamC^LC58 pupae.

Expression of LamC^Scer\UAS.cSa under the control of Scer\GAL4^sr-md710 rescues head eversion in 56% of LamC^LC58 pupae. Overall muscle morphology is restored to normal in these pupae.

Expression of LamC^Scer\UAS.cSa under the control of Scer\GAL4^how-24B rescues 89% of LamC^LC58 animals to viability.

Expression of LamC^Scer\UAS.cSa under the control of Scer\GAL4^shot-NP3076 rescues 60% of LamC^LC58 animals to viability, while a further 23% of the animals show rescued head eversion but die as pupae.

(Uchino et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

LamC^Scer\UAS.cSa

LamC^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Schulze

(Schulze et al., 2005)

Secondary FlyBase IDs

References (17)

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