FlyBase Allele Report: Dmel\eIF4A[YE9]

General Information

Symbol

Dmel\eIF4A^YE9

Species

D. melanogaster

Name

FlyBase ID

FBal0192049

Feature type

allele

Associated gene

Dmel\eIF4A

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Li et al., 2005)

Mutagen

ethyl methanesulfonate

(Li et al., 2005)

Progenitor genotype

Cytology

Description

Amino acid replacement: R321H. Nucleotide substitution: G1027A.

(Li et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:5,984,837..5,984,837 [+]

Nucleotide change:

G5984837A

Reported nucleotide change:

G1027A

Amino acid change:

Reported amino acid change:

R321H.

(Li et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | dominant | larval stage

(Li et al., 2005)

lethal (with Df(2L)Gpdh1-A)

(Li et al., 2005)

lethal | embryonic stage (with Df(2L)E110)

(Li et al., 2005)

lethal | first instar larval stage (with Df(2L)E110)

(Li et al., 2005)

lethal | first instar larval stage (with eIF4A⁰²⁴³⁹)

(Li et al., 2005)

lethal | second instar larval stage (with eIF4A⁰²⁴³⁹)

(Li et al., 2005)

lethal - all die during embryonic stage | recessive

(Li et al., 2005)

some die during embryonic stage | recessive

(Li et al., 2005)

Phenotype Manifest In

amnioserosa

(Li et al., 2005)

cephalopharyngeal skeleton

(Li et al., 2005)

embryonic/first instar larval cuticle

(Li et al., 2005)

Detailed Description

Statement

Reference

Homozygous embryos have severely defective head skeletons, mild segmentation defects in the cuticle and an increased number of amnioserosa cells. eIF-4a^YE9/Df(2L)E110 animals show late embryonic and first larval instar lethality, and have very few cuticle defects. Embryos derived from a cross of heterozygous females to heterozygous males show a hatching rate of only 72.3%, while the hatching rate of embryos derived from a cross of eIF-4a^YE9/+ females to Df(2L)E110/+ males is 78.3%. Heterozygous larvae show a growth delay, eclosing 2 days later than their control siblings at 25^oC.

(Li et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

eIF-4a[+]/eIF4A^YE9 is a suppressor of lethal | dominant phenotype of dpp^H46

(Li et al., 2005)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Dominant suppressor of the haplo-insufficiency of dpp^H46, with eIF-4a^YE9 being required both maternally and zygotically for the suppression to occur.

(Li et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

eIF4A^24A03

(Li et al., 2005)

eIF4A^09A09

(Li et al., 2005)

eIF4A^10A07

(Li et al., 2005)