FlyBase Allele Report: Dmel\insv[23B]

General Information

Symbol

Dmel\insv^23B

Species

D. melanogaster

Name

FlyBase ID

FBal0193099

Feature type

allele

Associated gene

Dmel\insv

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Reeves and Posakony, 2005)

Progenitor genotype

P{SUPor-P}KG07404

(Reeves and Posakony, 2005)

Cytology

Description

Imprecise excision of P{SUPor-P}KG07404 has deleted sequence from the P{SUPor-P}KG07404 insertion site into the insv coding region including all of the first exon and over half of the second.

(Reeves and Posakony, 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Duan et al., 2011)

visible

(Duan et al., 2011)

visible (with insv^23I)

(Duan et al., 2011)

Phenotype Manifest In

adult abdominal sensillum

(Duan et al., 2011)

adult abdominal sensillum (with insv^23I)

(Duan et al., 2011)

macrochaeta, with Scer\GAL4^pnr.PU

(Coumailleau and Schweisguth, 2014)

microchaeta

(Duan et al., 2011)

tormogen cell | ectopic

(Duan et al., 2011)

tormogen cell | ectopic (with insv^23I)

(Duan et al., 2011)

trichogen cell

(Duan et al., 2011)

trichogen cell (with insv^23I)

(Duan et al., 2011)

Detailed Description

Statement

Reference

insv^23B mutant flies have no detectable macrochaete bristle phenotype.

(Coumailleau and Schweisguth, 2014)

Homozygous adults show a grossly normal patterning of the notum mechanosensory organs, although careful quantitation reveals that there are approximately 10% fewer notum microchaetae in the homozygotes compared to wild type. Homozygous and insv^23B/insv^23I adults consistently have a small number of double-socketed sensory organs on the abdomen, with the shaft cells having been replaced by socket cells.

(Duan et al., 2011)

FlyBase curator comment: The strong sensory organ defects reported in FBrf0183881 for insv^23B mutant clones have subsequently been shown to be due to a second-site mutation in l(2)gl that is also present on the mutant chromosome (see FBrf0207678).

(Reeves and Posakony, 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

H^E31, insv^23B has visible | dominant phenotype, enhanceable by insb^Δ1/Tes^+t22/qkr54B^+t22/CG14478^+t22

(Coumailleau and Schweisguth, 2014)

Suppressed by

Statement

Reference

insv^23B has visible phenotype, suppressible by N^l1N-ts1

(Duan et al., 2011)

Enhancer of

Statement

Reference

insv^23B is an enhancer of visible | dominant phenotype of H^E31

(Coumailleau and Schweisguth, 2014)

Suppressor of

Statement

Reference

insv^23B is a suppressor of visible phenotype of N^l1N-ts1

(Duan et al., 2011)

NOT Suppressor of

Statement

Reference

insv^23B is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^sd.PU, insb^UAS.GFP

(Coumailleau and Schweisguth, 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

H^E31, insv^23B has macrochaeta phenotype, enhanceable by insb^Δ1/Tes^+t22/qkr54B^+t22/CG14478^+t22

(Coumailleau and Schweisguth, 2014)

Suppressed by

Statement

Reference

insv^23B has adult abdominal sensillum phenotype, suppressible by N^l1N-ts1

(Duan et al., 2011)

insv^23B has tormogen cell | ectopic phenotype, suppressible by N^l1N-ts1

(Duan et al., 2011)

insv^23B has trichogen cell phenotype, suppressible by N^l1N-ts1

(Duan et al., 2011)

Enhancer of

Statement

Reference

insv^23B is an enhancer of macrochaeta phenotype of H^E31

(Coumailleau and Schweisguth, 2014)

Suppressor of

Statement

Reference

insv^23B is a suppressor of microchaeta | increased number phenotype of N^l1N-ts1

(Duan et al., 2011)

NOT Suppressor of

Statement

Reference

insv^23B is a non-suppressor of wing phenotype of Scer\GAL4^sd.PU, insb^UAS.GFP

(Coumailleau and Schweisguth, 2014)

Additional Comments

Genetic Interactions

Statement

Reference

insv^23B enhances the double-socket macrochaetae phenotype seen in H^E31/+ mutants. The bristle loss phenotype is further enhanced by loss of insb, using insb^Δ1, in which the function of CG14478, Tes and qkr54B has been restored using P{CG14478-Tes-qkr54B-mCherry} (which comprises of the CG14478^{T:Disc\RFP-mCherry}, Tes^{T:Disc\RFP-mCherry} and qkr54B^{T:Disc\RFP-mCherry} alleles).

No detectable macrochaetae phenotypes are observed in insv^23B, insb^Δ1 double mutant flies in which the function of CG14478, Tes and qkr54B has been restored using P{CG14478-Tes-qkr54B-mCherry} (which comprises of the CG14478^{T:Disc\RFP-mCherry}, Tes^{T:Disc\RFP-mCherry} and qkr54B^{T:Disc\RFP-mCherry} alleles).

insv^23B does not suppress the wing defects seen when insb^{Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^sd.PU.

(Coumailleau and Schweisguth, 2014)

The ectopic microchaetae seen on the notum in N^l1N-ts1 females raised at 22[o]C are suppressed by insv^23B.

The double-socketed sensory organs on the abdomen which are seen in insv^23B adults are largely suppressed by N^l1N-ts1 at 25[o]C.

(Duan et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

insv^23B is rescued by insv^+t3.67

(Duan et al., 2011)

Comments

The mild reduction in the number of microchaetae that is seen on the notum of insv^23B homozygotes is rescued by insv^+t3.67.

(Duan et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

The stock contains a second-site deletion uncovering l(2)gl (determined using complementation and PCR tests).

(Roegiers et al., 2009)

The insv^23B mutant chromosome has been cleaned up to remove the second-site mutation in l(2)gl which was shown in FBrf0183881 to be present on the chromosome.

(Duan et al., 2011)

The sensory organ defects reported in FBrf0183881 to be due to the insv^23B allele can in fact be accounted for by the second-site mutation in l(2)gl that is also present on the chromosome; the sensory organ defects seen in clones of the "insv[23B]" chromosome are completely rescued by expression of l(2)gl^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^{neur-GAL4-A101}.

(Roegiers et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

23B

(Reeves and Posakony, 2005)

CG3227^23B

insv^23B

(Mukherjee et al., 2022, Fedotova et al., 2019, Ueberschär et al., 2019, Fedotova et al., 2018, Dai et al., 2015, Coumailleau and Schweisguth, 2014, Duan et al., 2011, Roegiers et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (9)

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