FB2024_03 , released June 25, 2024
Allele: Dmel\nAChRα6DAS1
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General Information
Symbol
Dmel\nAChRα6DAS1
Species
D. melanogaster
Name
FlyBase ID
FBal0193528
Feature type
allele
Associated gene
Dmel\nAChRα6
Associated Insertion(s)
Carried in Construct
Also Known As
Dα6DAS1, nAcRα-30DDAS1
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Watson et al., 2010, Cook, 2006.12.5)
Progenitor genotype
Cytology
Description

G to A transition at the first nucleotide of intron 1, which alters the splice donor site. This is predicted to generate a peptide truncated after exon 1 with 22 extraneous amino acids at the C-terminus encoded by intronic sequences.

(Watson et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:9,869,531..9,869,531 [-]
Nucleotide change:

G9869531A

Reported nucleotide change:

G?A

Comment:

G to A transition at the first nucleotide of intron 1, which alters the splice donor site. This is predicted to generate a peptide truncated after exon 1 with 22 extraneous amino acids at the C-terminus encoded by intronic sequences. The first coding exon was counted as exon 1.

(Watson et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  neurofibromatosis
      modeled by Nf1E2
      (Walker et al., 2013)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutant flies show increased resistance to the nAChRα6 agonist spinosad compared to wild-type controls.

      (Nakayama et al., 2014)

      Mutant adults are specifically resistant to spinosyns and synthetically-modified spinosyns (spinosyn A, spinosad and spinetoram) compared to controls, but are not resistant to other insecticides (imidacloprid, acetamiprid, thiamethoxam, methyllycaconitine, emamectin, avermectin B[[1a]], permethrin and indoxacarb).

      Homozygous larvae display no overt differences in firing patterns of isolated ventral ganglia compared to wild type, with substantial levels of spontaneous firing of action potentials being observed in both cases. In the presence of 100 nM spinosyn A, wild-type ganglia respond with an increase in neuronal firing (9 out of 9 tested ganglia), while homozygous ganglia produce no response to this concentration of spinosyn A. Both wild-type and homozygous ganglia are capable of responding to higher concentrations of spinosyn A (10μM).

      (Watson et al., 2010)

      nAcRα-30DDAS1 mutants exhibit resistance to the insecticide spinosad.

      (Cook, 2006.12.5)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      NOT suppressed by
      Enhancer of
      Statement
      Reference

      nAChRα6DAS1 is an enhancer of decreased body size | pupal stage phenotype of Nf1E2

      (Walker et al., 2013)
      Phenotype Manifest In
      NOT Enhanced by
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      nAChRα6DAS1 is partially rescued by nAChRα6UAS.RE/Scer\GAL4P2.4.Pdf

      (Rosenthal et al., 2021)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      Reported As
      Symbol Synonym
      Dα6DAS1
      (Nakayama et al., 2023, Rosenthal et al., 2021, Nakayama et al., 2016, Nakayama et al., 2014)
      nAChRa-30DDAS1
      (Ghezzi et al., 2013)
      nAChRalpha6DAS1
      (Bazzi et al., 2023)
      nAChRα6DAS1
      nAcRalpha-30DDAS1
      (Cook, 2006.12.5)
      nAcRα-30DDAS1
      (Ghezzi et al., 2013, Walker et al., 2013, Watson et al., 2010)
      rsn1
      (Watson et al., 2010)
      rsn2
      (Watson et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (9)