FB2024_03 , released June 25, 2024
Allele: Dmel\eysUAS.cZa
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General Information
Symbol
Dmel\eysUAS.cZa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Zelhof
FlyBase ID
FBal0193673
Feature type
allele
Associated gene
Dmel\eys
Associated Insertion(s)
Carried in Construct
P{UAS-spam.Z}
Also Known As
UAS-spam
Key Links
Transgenic product class
wild_type
(Zelhof et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Zelhof et al., 2006)
Mutagen
in vitro construct
(Zelhof et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-spam.Z}
Cytology
Description

UASt regulatory sequences drive expression of eys.

(Zelhof et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
eys
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of eysScer\UAS.cZa in eyes, under the control of Scer\GAL4GMR.PF, markedly expands the inter-rhabdomeral space compared to wild-type eyes.

      Expression of eysScer\UAS.cZa, under the control of Scer\GAL4hs.PB, where eysScer\UAS.cZa expression is induced by a heat shock at 36-64 hours after puparium formation, results in the development of broken-up rhabdomeres in the eye.

      Expression of eysScer\UAS.cZa in the ocellus, driven by Scer\GAL4hs.PB at 29oC, results in the development of a novel inter-rhabdomeral space surrounding otherwise structurally intact rhabdomeres. Stronger expression of eysScer\UAS.cZa in the ocellus, driven by Scer\GAL4GMR.PF, results in the generation of an extensive inter-rhabdomeral space accompanied by the disintegration of the rhabdomeres into their individual microvilli.

      (Zelhof et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      eysUAS.cZa/Scer\GAL4hs.PB rescues eys2649

      (Cook et al., 2008)
      Partially rescues

      eysUAS.cZa/Scer\GAL4hs.PB partially rescues eys1

      (Zelhof et al., 2006)

      Scer\GAL4ninaE.PD/eysUAS.cZa partially rescues eys1

      (Zelhof et al., 2006)
      Comments

      The mechanosensory defects seen in the bristles and Johnston's organ of eys2649 flies at 37[o]C are rescued by expression of eysScer\UAS.cZa under the control of Scer\GAL4hs.PB.

      (Cook et al., 2008)

      Expression of eysScer\UAS.cZa under the control of Scer\GAL4hs.PB fully rescues the rhabdomere phenotype of eys1 flies when eysScer\UAS.cZa expression is induced at 36-64 hours after puparium formation. Induction at about 72 hours, using either Scer\GAL4hs.PB or Scer\GAL4ninaE.PD, leads only to a partial rescue.

      (Zelhof et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in a plasmid and transfected into S2 cells to study the localization of the expressed protein.

      (Zelhof et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      eysScer\UAS.cZa
      eysUAS.cZa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Zelhof
      Secondary FlyBase IDs
        References (4)