FB2024_03 , released June 25, 2024
Allele: Dmel\BEAF-32BID.UAS.Tag:HA,Tag:NLS(SV40-largeT)
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General Information
Symbol
Dmel\BEAF-32BID.UAS.Tag:HA,Tag:NLS(SV40-largeT)
Species
D. melanogaster
Name
FlyBase ID
FBal0194347
Feature type
allele
Associated gene
Dmel\BEAF-32
Associated Insertion(s)
Carried in Construct
P{UAS-BEAF-32.BID}
Key Links
Transgenic product class
dominant_negative_variant
(Gilbert et al., 2006)
inframe_deletion
(Gilbert et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Gilbert et al., 2006)
inframe_deletion
(Gilbert et al., 2006)
Mutagen
in vitro construct
(Gilbert et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-BEAF-32.BID}
Cytology
Description

UAS regulatory sequences drive expression of the C-terminal 141 amino acid residues of BEAF-32 which are tagged at the N-terminal end with a Tag:HA tag and a Tag:NLS(SV40-largeT) nuclear localisation signal. Encodes a dominant negative form of BEAF-32.

(Gilbert et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
BEAF-32
Tagged with
Tag:HA
Tag:NLS(SV40-largeT)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of BEAF-32BID.UAS.Tag:HA,Tag:NLS(SV40-largeT) under the control of Scer\GAL4GMR.long causes an increase in the proportion of pale R8 photoreceptors and a reciprocal decrease in the proportion of yellow R8 photoreceptors, compared to controls.

      (Jukam et al., 2016)

      Expression of BEAF-32BID.Scer\UAS.T:Ivir\HA1,T:SV40\nls2 under the control of Scer\GAL4ey.PH results in a rough eye phenotype.

      (Roy et al., 2011)

      Expression of BEAF-32BID.Scer\UAS.T:Ivir\HA1,T:SV40\nls2 under the control of Scer\GAL4da.G32 can result in lethality, depending on the P{UAS-BEAF-32.BID} insertion line used (the severity of the phenotype depends on the level of expression from the transgene).

      Expression of BEAF-32BID.Scer\UAS.T:Ivir\HA1,T:SV40\nls2 under the control of Scer\GAL4ey.PH results in a rough eye phenotype. The severity of the phenotype is increased if the flies carry two copies of BEAF-32BID.Scer\UAS.T:Ivir\HA1,T:SV40\nls2.

      Third instar larvae expressing BEAF-32BID.Scer\UAS.T:Ivir\HA1,T:SV40\nls2 under the control of Scer\GAL4AB1 or Scer\GAL434B have smaller salivary glands than normal which are easily fragmented and lack the characteristic well-defined banding pattern.

      The mitotic index in the brain is not altered compared to wild type in larvae expressing BEAF-32BID.Scer\UAS.T:Ivir\HA1,T:SV40\nls2 under the control of Scer\GAL4c768. No defects in chromosome condensation are seen, and there is no premature sister-chromatid separation, aneuploidy or anaphase problems. The size of the brain is normal in these larvae.

      (Gilbert et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      BEAF-32BID.UAS.Tag:HA,Tag:NLS(SV40-largeT), Scer\GAL4ey.PH has visible phenotype, enhanceable by ftz[+]/ftz3

      (Roy et al., 2011)
      NOT Enhanced by
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      BEAF-32BID.UAS.Tag:HA,Tag:NLS(SV40-largeT), Scer\GAL4ey.PH has eye phenotype, enhanceable by ftz[+]/ftz3

      (Roy et al., 2011)
      NOT Enhanced by
      Statement
      Reference

      BEAF-32BID.UAS.Tag:HA,Tag:NLS(SV40-largeT), Scer\GAL4ey.PH has eye phenotype, non-enhanceable by Dref[+]/DrefKG09294

      (Roy et al., 2011)

      BEAF-32BID.UAS.Tag:HA,Tag:NLS(SV40-largeT), Scer\GAL4ey.PH has eye phenotype, non-enhanceable by mle[+]/mle9

      (Roy et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The rough eye phenotype of flies expressing BEAF-32BID.Scer\UAS.T:Ivir\HA1,T:SV40\nls2 under the control of Scer\GAL4ey.PH is enhanced by ftz3/+, but is not enhanced by either DrefKG09294/+ or mle9/+.

      (Roy et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      BEAF-32BID.Scer\UAS.T:Ivir\HA1,T:SV40\nls-largeT
      BEAF-32BID.Scer\UAS.T:Ivir\HA1,T:SV40\nls2
      BEAF-32BID.UAS.Tag:HA,Tag:NLS(SV40-largeT)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)