FB2024_03 , released June 25, 2024
Allele: Dmel\Rtf1RNAi.Sym.UAS
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General Information
Symbol
Dmel\Rtf1RNAi.Sym.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0195510
Feature type
allele
Associated gene
Dmel\Rtf1
Associated Insertion(s)
Carried in Construct
P{Sym-UAS-Rft1}
Key Links
Transgenic product class
RNAi_reagent
(Tenney et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Tenney et al., 2006)
Mutagen
in vitro construct
(Tenney et al., 2006)
Progenitor genotype
Carried in construct
P{Sym-UAS-Rft1}
Cytology
Description

Sense-antisense transcription (driven by two convergent arrays of UAS regulatory sequences) of a 600bp (nucleotides 841-1440) EcoRI-BglII fragment amplified from a Rtf1 cDNA results in double stranded RNA (dsRNA) expression.

(Tenney et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Rtf1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4Act5C.PU-driven expression of Rtf1dsRNA.Sym.Scer\UAS, from either of two different P{Sym-UAS-Rft1} insertions, results in pupal lethality.

      Scer\GAL4hs.PB-driven co-expression of Rtf1dsRNA.Sym.Scer\UAS, from either of two different P{Sym-UAS-Rft1} insertions, at 27[o]C is non-lethal.

      Scer\GAL4Act5C-driven co-expression of Rtf1Sym.Scer\UAS and Rtf1a.Sym.Scer\UAS, from either of two different P{Sym-UAS-Rft1} insertions, results in pupal lethality.

      Scer\GAL4hs.PB-driven co-expression of Rtf1Sym.Scer\UAS and Rtf1a.Sym.Scer\UAS, from either of two different P{Sym-UAS-Rft1} insertions, at 27oC is non-lethal.

      (Tenney et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      Rtf1RNAi.Sym.UAS/Scer\GAL4hs.PB is an enhancer of visible phenotype of Nnd-1

      (Tenney et al., 2006)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Rtf1RNAi.Sym.UAS/Scer\GAL4hs.PB is an enhancer of wing phenotype of Nnd-1

      (Tenney et al., 2006)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Rtf1Sym.Scer\UAS and Rtf1a.Sym.Scer\UAS under the control of Scer\GAL4hs.PB, from either of two different P{Sym-UAS-Rft1} insertions, at 27oC significantly decreases the width of Nnd-1 wings relative to controls.

      Co-expression of Rtf1dsRNA.Sym.Scer\UAS under the control of Scer\GAL4hs.PB, from either of two different P{Sym-UAS-Rft1} insertions, at 27[o]C significantly decreases the width of Nnd-1 wings relative to controls.

      (Tenney et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Associated with: Rtf1a.Sym.Scer\UAS

      (Tenney et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Rtf1RNAi.Sym.UAS
      Rtf1Sym.Scer\UAS
      Rtf1dsRNA.Sym.Scer\UAS
      Rtf1dsRNA.Sym.UAS
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0195511
      References (2)