FB2024_03 , released June 25, 2024
Allele: Dmel\Pink1UAS.cCa
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General Information
Symbol
Dmel\Pink1UAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Clark
FlyBase ID
FBal0196298
Feature type
allele
Associated gene
Dmel\Pink1
Associated Insertion(s)
Carried in Construct
P{UAS-Pink1.C}
Also Known As
UAS-pink1
Key Links
Transgenic product class
wild_type
(Clark et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Clark et al., 2006)
Mutagen
in vitro construct
(Clark et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Pink1.C}
Cytology
Description

UAS regulatory sequences drive expression of Pink1.

(Clark et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Pink1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Parkinson's disease 6
      CEA
      (Kandul et al., 2016)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  neurodegenerative disease
      modeled by Tom40GD5210
      (Liu et al., 2018)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing Pink1UAS.cCa under the control of Scer\GAL4Cg.PA does not seem to affect mitochondria size in the third instar larval fat body (as assessed by MitoGFP).

      Expressing Pink1UAS.cCa under the control of Scer\GAL4Mef2.PR leads to a small but significant decrease in adult climbing ability; adult thorax muscles do not show obvious signs of degeneration. In 3-day old adults, muscle mitochondria do not show obvious defects; in 35-days old adults, however, mitochondria are slightly smaller, rounder and/or of irregular size, and cristae exhibit mild abnormalities.

      (Zhou et al., 2019)

      The expression of Pink1UAS.cCa under the control of Scer\GAL4Cg.PA does not affect the number of autophagosome-like structures (Atg8a-positive) in the early third instar larval fat body, as compared to controls; in TEM, the mitochondria number and morphology are not dramatically changed, as compared to controls.

      The expression of Pink1UAS.cCa under the control of Scer\GAL4GMR.PU does not lead to eye defects (rhabdomere number in both 2- and 30-days old adults), as compared to controls.

      (Liu et al., 2018)

      The expression of Pink1Scer\UAS.cCa under the control of Scer\GAL4Act.PU in clones within mosaic midguts results in enterocytes exhibiting a significant decrease in the relative mitochondrial content during pupariation, as compared to non-clone enterocytes; expression under the control of Scer\GAL4esg.PU results in a significant decrease in the relative mitochondrial content of midgut intestinal stem cells, from late third instar larval until afer puparium formation, but not at early third instar larval stage or adult stage, as compared to controls.

      (Liu et al., 2016)

      Expression of Pink1Scer\UAS.cCa under the control of Scer\GAL4GMR.PF at 18[o]C results in a mild rough eye phenotype. A rough eye phenotype is also seen at 29[o]C.

      (Yun et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Pink1UAS.cCa, Scer\GAL4GMR.PF has visible phenotype, enhanceable by HtrA2UAS.cYa, Scer\GAL4GMR.PF

      (Yun et al., 2008)
      NOT Enhanced by
      Statement
      Reference

      Pink1UAS.cCa, Scer\GAL4GMR.PF has visible phenotype, non-enhanceable by HtrA2S266A.UAS, Scer\GAL4GMR.PF

      (Yun et al., 2008)
      NOT suppressed by
      Statement
      Reference

      Pink1UAS.cCa, Scer\GAL4GMR.PF has visible phenotype, non-suppressible by HtrA2RNAi.UAS.cYa, Scer\GAL4GMR.PF

      (Yun et al., 2008)
      Enhancer of
      Suppressor of
      Statement
      Reference

      Pink1UAS.cCa/Scer\GAL4ple.PF is a suppressor of decreased rate of adult locomotory behavior phenotype of CreldΔ51

      (Paradis et al., 2022)

      Pink1UAS.cCa, Scer\GAL4ple.PF is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Pink1UAS.cCa, Scer\GAL4ple.PF is a suppressor of decreased cell number | adult stage | progressive phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Pink1UAS.cCa, Scer\GAL4ple.PF is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Pink1UAS.cCa, Scer\GAL4ple.PF is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Pink1UAS.cCa, Scer\GAL4ple.PF is a suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Pink1UAS.cCa, Scer\GAL4ple.PF is a suppressor of decreased cell number | adult stage phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Pink1UAS.cCa, Scer\GAL4Cg.PA is a suppressor | partially of abnormal size | third instar larval stage phenotype of DhprHMC05508, Scer\GAL4Cg.PA

      (Zhou et al., 2019)

      Pink1UAS.cCa, Scer\GAL4Mef2.PR is a suppressor of abnormal size | adult stage phenotype of DhprHMC05508, Scer\GAL4Mef2.PR

      (Zhou et al., 2019)

      Pink1UAS.cCa, Scer\GAL4Mef2.PR is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4Mef2.PR, cluGD13926

      (Wang et al., 2016)

      Pink1UAS.cCa, Scer\GAL4Mef2.PR is a suppressor of partially lethal - majority die phenotype of Scer\GAL4Mef2.PR, cluGD13926

      (Wang et al., 2016)
      NOT Suppressor of
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Pink1UAS.cCa, Scer\GAL4GMR.PF has eye phenotype, enhanceable by HtrA2UAS.cYa, Scer\GAL4GMR.PF

      (Yun et al., 2008)
      NOT Enhanced by
      Statement
      Reference

      Pink1UAS.cCa, Scer\GAL4GMR.PF has eye phenotype, non-enhanceable by HtrA2S266A.UAS, Scer\GAL4GMR.PF

      (Yun et al., 2008)
      NOT suppressed by
      Statement
      Reference

      Pink1UAS.cCa, Scer\GAL4GMR.PF has eye phenotype, non-suppressible by HtrA2RNAi.UAS.cYa, Scer\GAL4GMR.PF

      (Yun et al., 2008)
      Enhancer of
      Suppressor of
      Statement
      Reference

      Pink1UAS.cCa, Scer\GAL4ple.PF is a suppressor of dopaminergic neuron | adult stage | decreased number phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Pink1UAS.cCa, Scer\GAL4ple.PF is a suppressor of dopaminergic neuron | adult stage | decreased number phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Pink1UAS.cCa, Scer\GAL4ple.PF is a suppressor of mitochondrion | adult stage phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Pink1UAS.cCa, Scer\GAL4Cg.PA is a suppressor | partially of mitochondrion | third instar larval stage phenotype of DhprHMC05508, Scer\GAL4Cg.PA

      (Zhou et al., 2019)

      Pink1UAS.cCa, Scer\GAL4Cg.PA is a suppressor | partially of embryonic/larval fat body | third instar larval stage phenotype of DhprHMC05508, Scer\GAL4Cg.PA

      (Zhou et al., 2019)

      Pink1UAS.cCa, Scer\GAL4Mef2.PR is a suppressor of adult somatic muscle cell of thorax | adult stage | progressive phenotype of DhprHMC05508, Scer\GAL4Mef2.PR

      (Zhou et al., 2019)

      Pink1UAS.cCa, Scer\GAL4Mef2.PR is a suppressor | partially of mitochondrion | adult stage | progressive phenotype of DhprHMC05508, Scer\GAL4Mef2.PR

      (Zhou et al., 2019)

      Pink1UAS.cCa, Scer\GAL4Mef2.PR is a suppressor | partially of mitochondrial crista | adult stage | progressive phenotype of DhprHMC05508, Scer\GAL4Mef2.PR

      (Zhou et al., 2019)

      Pink1UAS.cCa, Scer\GAL4Cg.PA is a suppressor | partially of fat cell | early third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030

      (Liu et al., 2018)

      Pink1UAS.cCa, Scer\GAL4Cg.PA is a suppressor | partially of autophagosome | early third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030

      (Liu et al., 2018)

      Pink1UAS.cCa, Scer\GAL4Mef2.PR is a suppressor of mitochondrion | adult stage phenotype of Scer\GAL4Mef2.PR, cluGD13926

      (Wang et al., 2016)
      NOT Suppressor of
      Statement
      Reference

      Pink1UAS.cCa, Scer\GAL4ple.PF is a non-suppressor of dopaminergic neuron | adult stage | decreased number phenotype of Hsap\ZNF746DM.UAS, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Pink1UAS.cCa/Atg5JF02703, Scer\GAL4Cg.PA is a non-suppressor of fat cell | early third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030

      (Liu et al., 2018)

      Pink1UAS.cCa/Syx17JF01937, Scer\GAL4Cg.PA is a non-suppressor of fat cell | early third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030

      (Liu et al., 2018)

      Pink1UAS.cCa/Atg5JF02703, Scer\GAL4Cg.PA is a non-suppressor of autophagosome | early third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030

      (Liu et al., 2018)

      Pink1UAS.cCa/Syx17JF01937, Scer\GAL4Cg.PA is a non-suppressor of autophagosome | early third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030

      (Liu et al., 2018)

      Pink1UAS.cCa/parkHMS01800, Scer\GAL4Cg.PA is a non-suppressor of fat cell | early third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030

      (Liu et al., 2018)

      Pink1UAS.cCa/Scer\GAL4Tub.PU is a non-suppressor of mitochondrion | third instar larval stage phenotype of MitofilinLL02849

      (Tsai et al., 2018)

      Pink1UAS.cCa/Scer\GAL4Tub.PU is a non-suppressor of mitochondrial crista | third instar larval stage phenotype of MitofilinLL02849

      (Tsai et al., 2018)

      Pink1UAS.cCa/Scer\GAL4Tub.PU is a non-suppressor of embryonic/larval hypodermal muscle cell | third instar larval stage phenotype of MitofilinLL02849

      (Tsai et al., 2018)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The larval locomotor deficit, very low adult eclosion rate as well as the mitochondrial morphology (enlarged clustered mitochondria) and functional (loss of inner mitochondrial potential measured using cationic fluorescent dye JC-1) defects in adult indirect flight muscles characteristic for animals expressing cluGD13926 under the control of Scer\GAL4Mef2.PR can be suppressed by co-expression of Pink1Scer\UAS.cCa.

      (Wang et al., 2016)

      Expression of HtrA2Scer\UAS.cYa enhances the rough eye phenotype seen when Pink1Scer\UAS.cCa is expressed under the control of Scer\GAL4GMR.PF at 18[o]C.

      Expression of HtrA2S266A.Scer\UAS does not enhance the rough eye phenotype seen when Pink1Scer\UAS.cCa is expressed under the control of Scer\GAL4GMR.PF at 18[o]C.

      Expression of HtrA2dsRNA.Scer\UAS.cYa fails to suppress the rough eye phenotype seen when Pink1Scer\UAS.cCa is expressed under the control of Scer\GAL4GMR.PF at 29[o]C.

      (Yun et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Pink1UAS.cCa/Scer\GAL4Mef2.PR rescues Pink15

      (Deng et al., 2008)

      Pink1UAS.cCa rescues Pink15

      (Yun et al., 2008)
      Comments

      Expression of Pink1Scer\UAS.cCa under the control of Scer\GAL4Mef2.PR completely rescues the defects in mitochondrial morphology that are seen in the indirect flight muscles of Pink15 mutants.

      (Deng et al., 2008)

      Expression of Pink1Scer\UAS.cCa under the control of Scer\GAL4Mef2.PR rescues the mitochondria morphology defects seen in Pink15 mutant indirect flight muscles.

      (Yun et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Pink1Scer\UAS.cCa
      Pink1UAS.cCa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Clark
      Secondary FlyBase IDs
        References (16)