FB2024_03 , released June 25, 2024
Allele: Dmel\Uba1B1
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General Information
Symbol
Dmel\Uba1B1
Species
D. melanogaster
Name
FlyBase ID
FBal0197417
Feature type
allele
Associated gene
Dmel\Uba1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Pfleger et al., 2007)
Progenitor genotype
Cytology
Description

Amino acid replacement: M184S.

(Pfleger et al., 2007)

Amino acid replacement: S181R.

(Pfleger et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
MNV
2R:9,692,489..9,692,490 [-]
Nucleotide change:

ATG9692491AGY

Amino acid change:

M184S | Uba1-PA; M1S | Uba1-PC

Reported amino acid change:

M184S

Comment:

The reported Met to Ser amino acid change (ATG to AGY) requires nucleotide changes at two positions of the codon. The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change. One of 2 missense mutations associated with this allele.

(Pfleger et al., 2007)
point_mutation
2R:9,692,500..9,692,500 [-]
Nucleotide change:

A9692500C

Amino acid change:

S181R | Uba1-PA

Reported amino acid change:

S181R

Comment:

Amino acid replacement reported as reported as Ser to Arg, which could result from a nucleotide substitution in the first or second base of the codon. The mutation was annotated at the first base of the codon. One of 2 missense mutations associated with this allele.

(Pfleger et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Uba1B1/+ mutant flies display a significant decrease in lifespan as compared to wild type.

      Very few Uba1B1/Uba1B1 mutants reach adulthood, and those that do are short lived, infertile, climbing defective, and display visible abnormalities including extra sternopleural bristles, loss of one or more dorsocentral bristles, ectopic wing veins, unusually thick wing veins, and abnormal wing posture.

      Very few Uba1B1/Uba1B2 mutants reach adulthood, and those that do are infertile, climbing defective, and display visible abnormalities including unusually thick wing veins.

      (Liu and Pfleger, 2013)

      Uba1B1 homozygous and Uba1B1/Uba1B2 trans-heterozygous eye discs exhibit a significant increase in proliferation (as observed through phosphorylated His3 signal). Ectopic proliferation posterior to the morphogenetic furrow is significant enough that the region of frequent anti-pHis3 staining extends two to three times as far into the region posterior to the morphogenetic furrow.

      Uba1B1 homozygous adults reveal occasional overgrowths, such as outgrowths on the leg, proboscis or humeral regions consistent with ectopic divisions.

      Only a small percentage of Uba1B1 homozygotes reach adulthood.

      (Yan et al., 2009)

      Homozygous clones in the eye have a growth advantage compared to surrounding wild-type tissue.

      (Pfleger et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Uba1B1 has short lived phenotype, suppressible | partially by Ras85De1B/Ras85D[+]

      (Liu and Pfleger, 2013)

      Uba1B1/Uba1B2 has lethal phenotype, suppressible by Ras85De1B/Ras85D[+]

      (Yan et al., 2009)

      Ras85De1B/Ras85D[+], Uba1B1/Uba1B2 has viable phenotype, suppressible by egrUAS.cMa/Scer\GAL4GMR.PF

      (Yan et al., 2009)
      NOT suppressed by
      Statement
      Reference

      Uba1B1/Uba1B2 has lethal phenotype, non-suppressible by Egfr[+]/Egfrk05115

      (Yan et al., 2009)

      Uba1B1/Uba1B2 has lethal phenotype, non-suppressible by drkk02401/drk[+]

      (Yan et al., 2009)

      Uba1B1/Uba1B2 has lethal phenotype, non-suppressible by Sose4G/Sos[+]

      (Yan et al., 2009)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Uba1B1/Uba1B2 has eye disc posterior to the morphogenetic furrow phenotype, suppressible by Ras85De1B/Ras85D[+]

      (Yan et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Ras85De1B/+ rescues the lifespan defect of Uba1B1/+ mutants, and partially rescues the lifespan defect of Uba1B1/Uba1B1 mutants.

      (Liu and Pfleger, 2013)

      The cell-autonomous overgrowth phenotype found in Uba1B1 homozygous and Uba1B1/Uba1B2 trans-heterozygous eye discs is suppressed in a Ras85De1B heterozygous background.

      A Ras85De1B/+ background dramatically suppresses the lethality found in Uba1B1/Uba1B2 homozygotes.

      Uba1B1/Uba1B2 eyes in a Ras85De1B heterozygous background show decreased suppression of egrScer\UAS.cMa (under the control of Scer\GAL4GMR.PF) induced cell death.

      Overall cell proliferation in Uba1B1/Uba1B2 eye discs is reduced in a Ras85De1B/+ background. Ectopic proliferation posterior to the morphogenetic furrow decreases and is restricted to a thin stripe.

      A Egfrk05115/+, drkk02401/+, or Sose4G/+ background does not suppress the ectopic cell divisions found in Uba1B1/Uba1B2 trans-heterozygous eye discs.

      (Yan et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Uba1B1
      (Liu and Pfleger, 2013, Yan et al., 2009, Pfleger et al., 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)