FlyBase Allele Report: Dmel\Uba1[B2]

General Information

Symbol

Dmel\Uba1^B2

Species

D. melanogaster

Name

FlyBase ID

FBal0197418

Feature type

allele

Associated gene

Dmel\Uba1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Pfleger et al., 2007)

Progenitor genotype

Cytology

Description

Amino acid replacement: P884L.

(Pfleger et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:9,689,750..9,689,750 [-]

Nucleotide change:

C9689750T

Amino acid change:

P884L | Uba1-PA; P701L | Uba1-PC

Reported amino acid change:

P884L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Pfleger et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior (with Uba1^B1)

(Liu and Pfleger, 2013)

increased occurrence of cell division | larval stage (with Uba1^B1)

(Yan et al., 2009)

lethal - all die before end of P-stage | recessive

(Liu and Pfleger, 2013)

short lived | dominant

(Liu and Pfleger, 2013)

sterile (with Uba1^B1)

(Liu and Pfleger, 2013)

visible (with Uba1^B1)

(Liu and Pfleger, 2013)

visible | somatic clone

(Pfleger et al., 2007)

Phenotype Manifest In

eye | somatic clone

(Pfleger et al., 2007)

eye disc posterior to the morphogenetic furrow

(Yan et al., 2009)

eye disc posterior to the morphogenetic furrow (with Uba1^B1)

(Yan et al., 2009)

retina | P-stage | somatic clone

(Pfleger et al., 2007)

wing vein (with Uba1^B1)

(Liu and Pfleger, 2013)

Detailed Description

Statement

Reference

Uba1^B2/+ mutant flies display a significant decrease in lifespan as compared to wild type.

Most Uba1^B2/Uba1^B2 mutants die during late larval or pupal stages. The few escapers are infertile and display , climbing defects and visible abnormalities including unusually thick wing veins.

(Liu and Pfleger, 2013)

Uba1^B2 homozygous and Uba1^B1/Uba1^B2 trans-heterozygous eye discs exhibit a significant increase in proliferation (as observed through phosphorylated His3 signal). Ectopic proliferation posterior to the morphogenetic furrow is significant enough that the region of frequent anti-pHis3 staining extends two to three times as far into the region posterior to the morphogenetic furrow.

(Yan et al., 2009)

Homozygous clones in the eye have a growth advantage compared to surrounding wild-type tissue at 18^oC. At 30^oC, homozygous clones in the eye result in overgrowth of surrounding wild-type tissue.

Homozygous clones in the pupal retina 46 hours after puparium formation contain additional interommatidial cells compared to control retinas (where these extra cells are eliminated by apoptosis during the mid-pupal phase).

(Pfleger et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Uba1^B1/Uba1^B2 has lethal phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Yan et al., 2009)

Ras85D^e1B/Ras85D[+], Uba1^B1/Uba1^B2 has viable phenotype, suppressible by egr^UAS.cMa/Scer\GAL4^GMR.PF

(Yan et al., 2009)

NOT suppressed by

Statement

Reference

Uba1^B1/Uba1^B2 has lethal phenotype, non-suppressible by Egfr[+]/Egfr^k05115

(Yan et al., 2009)

Uba1^B1/Uba1^B2 has lethal phenotype, non-suppressible by drk^k02401/drk[+]

(Yan et al., 2009)

Uba1^B1/Uba1^B2 has lethal phenotype, non-suppressible by Sos^e4G/Sos[+]

(Yan et al., 2009)

Suppressor of

Statement

Reference

Uba1^B2/Uba1^B2 is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Pfleger et al., 2007)

Uba1^B2/Uba1^B2 is a suppressor of visible phenotype of hid^GMR.PG

(Pfleger et al., 2007)

Uba1^B2/Uba1^B2 is a suppressor of visible phenotype of grim^GMR.PC

(Pfleger et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Uba1^B1/Uba1^B2 has eye disc posterior to the morphogenetic furrow phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Yan et al., 2009)

Suppressor of

Statement

Reference

Uba1^B2/Uba1^B2 is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Pfleger et al., 2007)

Uba1^B2/Uba1^B2 is a suppressor of eye phenotype of hid^GMR.PG

(Pfleger et al., 2007)

Uba1^B2/Uba1^B2 is a suppressor of eye phenotype of grim^GMR.PC

(Pfleger et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The cell-autonomous overgrowth phenotype found in Uba1^B2 homozygous and Uba1^B1/Uba1^B2 trans-heterozygous eye discs is suppressed in a Ras85D^e1B heterozygous background.

A Ras85D^e1B/+ background dramatically suppresses the lethality found in Uba1^B1/Uba1^B2 homozygotes.

Uba1^B1/Uba1^B2 eyes in a Ras85D^e1B heterozygous background show decreased suppression of egr^Scer\UAS.cMa (under the control of Scer\GAL4^GMR.PF) induced cell death.

Overall cell proliferation in Uba1^B1/Uba1^B2 eye discs is reduced in a Ras85D^e1B/+ background. Ectopic proliferation posterior to the morphogenetic furrow decreases and is restricted to a thin stripe.

A Egfr^k05115/+, drk^k02401/+, or Sos^e4G/+ background does not suppress the ectopic cell divisions found in Uba1^B1/Uba1^B2 trans-heterozygous eye discs.

(Yan et al., 2009)

The loss of eye tissue that is caused by expression of egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF is partially suppressed if the eye is also homozygous for Uba1^B2.

The loss of eye tissue that is caused by W^GMR.PG is partially suppressed if the eye is also homozygous for Uba1^B2.

The loss of eye tissue that is caused by grim^GMR.PC is partially suppressed if the eye is also homozygous for Uba1^B2.

(Pfleger et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Uba1⁰³⁴⁰⁵

(Pfleger et al., 2007)

Uba1^s3484

(Pfleger et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Uba1^B2

(Liu and Pfleger, 2013, Yan et al., 2009, Pfleger et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (3)

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