FlyBase Allele Report: Dmel\Ddc[GD1189]

General Information

Symbol

Dmel\Ddc^GD1189

Species

D. melanogaster

Name

FlyBase ID

FBal0206829

Feature type

allele

Associated gene

Dmel\Ddc

Associated Insertion(s)

Carried in Construct

P{GD1189}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1189}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1189

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

abnormal flight, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

abnormal medium-term memory | adult stage | conditional, with Scer\GAL4^VT064246

(Muria et al., 2021)

abnormal memory, with Scer\GAL4⁵⁰¹⁵, Scer\GAL80^ts.αTub84B

(Lee et al., 2011)

abnormal memory, with Scer\GAL4^c316, Scer\GAL80^ts.αTub84B

(Lee et al., 2011)

abnormal memory, with Scer\GAL4^VT064246, Scer\GAL80^ts.αTub84B

(Lee et al., 2011)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^en.PU

(Anh et al., 2011)

Detailed Description

Statement

Reference

Temporal expression of Ddc^GD1189 under the control of Scer\GAL4^VT064246 during adulthood (by using Gal80[ts]) decreases olfactory medium term memory (1h) when the experiment is carried out in crowded conditions (groups of 32 flies - socially facilitated memory).

(Muria et al., 2021)

Expression of Ddc^GD1189 using Scer\GAL4^en.PU results in a paler posterior compartment. A held-out wing phenotype is not observed.

(Anh et al., 2011)

Adult flies expressing Ddc^KK100293 under the control of Scer\GAL4^c316 (with developmental expression suppressed by Scer\GAL80^ts.αTub84B and keeping them at 18[o]C until eclosion) exhibit normal olfactory learning but show a significant defect in 3 hour memory. In contrast, adult flies kept continuously at 18[o]C exhibit normal learning and 3 hour memory.

Flies expressing Ddc^KK100293 under the control of Scer\GAL4⁵⁰¹⁵ exhibit 3 hour memory impairment after training, independent of a cold shock.

Serotonin production is reduced significantly in flies expressing Ddc^KK100293 under the control of Scer\GAL4^elav-C155.

Spatial specificity for the requirement of Ddc in the DPM neurons is confirmed through expression of Ddc^KK100293 in DPM neurons under the control of Scer\GAL4^VT64246.

(Lee et al., 2011)

Adults expressing Ddc^GD1189 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing Ddc^GD1189 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 80-90% or 90-100% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1189

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Ddc^GD1189, Hn^GD12227, Scer\GAL4^G0431 has abnormal memory phenotype

(Chen et al., 2012)

Ddc^GD1189, Hn^GD12227, Scer\GAL4^c316, Scer\GAL80^ts.αTub84B has abnormal memory phenotype

(Lee et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

1 day memory is impaired after spaced training, but not after massed training in an olfactory associative conditioning assay in flies co-expressing Ddc^GD1189 and Hn^GD12227 under the control of Scer\GAL4^G0431. The defect in memory after spaced training is still seen if the temperature sensitive Scer\GAL80^ts.αTub84B allele is used to restrict Scer\GAL4 expression to the adult stage.

(Chen et al., 2012)

Temporal induction after development of both Ddc^KK100293 and Hn^GD12227 in Scer\GAL4^c316 neurons impairs anesthesia-resistant memory formation.

(Lee et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v3329

w¹¹¹⁸ P{GD1189}v3329

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1189

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ddc^GD1189

Ddc^RNAi

(Chen et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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