FlyBase Allele Report: Dmel\Dp[GD4444]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Dmel\Dp^GD4444

General Information

Symbol

Dmel\Dp^GD4444

Species

D. melanogaster

Name

FlyBase ID

FBal0206891

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Also Known As

UAS-Dp-RNAi, Dp RNAi

Key Links

Genomic Maps

Transgenic product class

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | progressive | spermatogenesis, with Scer\GAL4^fng-11-26

(Herrera et al., 2021)

decreased cell number | progressive | spermatogenesis, with Scer\GAL4^NP1624

(Herrera et al., 2021)

decreased cell number | spermatogenesis, with Scer\GAL4^A3.eya

(Herrera et al., 2021)

decreased cell number | spermatogenesis, with Scer\GAL4^C587

(Herrera et al., 2021)

decreased cell number | spermatogenesis, with Scer\GAL4^NP1624, Scer\GAL80^{hh-MI10526-T3XG80.0}

(Herrera et al., 2021)

decreased size | third instar larval stage, with Scer\GAL4^Mef2.PR

(Zappia et al., 2021)

lethal - all die before end of P-stage, with Scer\GAL4^Mef2.PR

(Zappia et al., 2021)

lethal - all die before end of P-stage | nutrition conditional, with Scer\GAL4^Cg.PA

(Zappia et al., 2021)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

majority die during pharate adult stage, with Scer\GAL4^Mef2.PR

(Zappia et al., 2021)

majority die during pupal stage, with Scer\GAL4^apolpp.PB

(Zappia et al., 2021)

majority die during pupal stage, with Scer\GAL4^Cg.PA

(Zappia et al., 2021)

melanotic mass phenotype | third instar larval stage | nutrition conditional, with Scer\GAL4^Mef2.PR

(Zappia et al., 2021)

partially lethal - majority die, with Scer\GAL4^apolpp.PB

(Zappia et al., 2021)

some die during pharate adult stage, with Scer\GAL4^apolpp.PB

(Zappia et al., 2021)

some die during pharate adult stage, with Scer\GAL4^Cg.PA

(Zappia et al., 2021)

some die during pupal stage, with Scer\GAL4^Mef2.PR

(Zappia et al., 2021)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

cyst progenitor cell | absent, with Scer\GAL4^NP1624

(Herrera et al., 2021)

hub cell, with Scer\GAL4^C587

(Herrera et al., 2021)

hub cell | absent, with Scer\GAL4^NP1624

(Herrera et al., 2021)

hub cell | decreased number, with Scer\GAL4^A3.eya

(Herrera et al., 2021)

hub cell | decreased number, with Scer\GAL4^C587

(Herrera et al., 2021)

hub cell | decreased number, with Scer\GAL4^fng-11-26

(Herrera et al., 2021)

hub cell | decreased number, with Scer\GAL4^NP1624

(Herrera et al., 2021)

hub cell | decreased number, with Scer\GAL4^NP1624, Scer\GAL80^{hh-MI10526-T3XG80.0}

(Herrera et al., 2021)

larval fat cell of larval fat body | third instar larval stage, with Scer\GAL4^Mef2.PR

(Zappia et al., 2021)

lipid droplet | third instar larval stage, with Scer\GAL4^Mef2.PR

(Zappia et al., 2021)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

muscle cell of ventral longitudinal muscle 3 | third instar larval stage, with Scer\GAL4^apolpp.PB

(Zappia et al., 2021)

muscle cell of ventral longitudinal muscle 3 | third instar larval stage, with Scer\GAL4^Cg.PA

(Zappia et al., 2021)

muscle cell of ventral longitudinal muscle 3 | third instar larval stage, with Scer\GAL4^Mef2.PR

(Zappia et al., 2021)

nucleus | increased number | third instar larval stage, with Scer\GAL4^apolpp.PB

(Zappia et al., 2021)

nucleus | increased number | third instar larval stage, with Scer\GAL4^Cg.PA

(Zappia et al., 2021)

nucleus | increased number | third instar larval stage, with Scer\GAL4^Mef2.PR

(Zappia et al., 2021)

testis, with Scer\GAL4^C587

(Herrera et al., 2021)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

Detailed Description

Statement

Reference

The expression of Dp^GD4444 under the control of Scer\GAL4^NP1624 alone leads to the complete loss of Cyst stem cells (only differentiated cyst cells are visible) and to a decrease in the number (or even absence) of hub cells in the testes; similarly, adulthood-only expression (under the additional control of Gal80{ts]) progressive leads to the complete loss of hub cells.

The expression of Dp^GD4444 under the control of Scer\GAL4^A3.eya or Scer\GAL4^fng-11-26 alone, or Scer\GAL4^NP1624 and Scer\GAL80^{hh-MI10526-T3XG80.0} combined induces a decrease in the number of hub cells in the testes.

The expression of babo^Q302D.UAS under the control of Scer\GAL4^C587 induces a decrease in the number of hub cells in the testes; unlike in controls, almost all testes show hub cell lineage-derived cells outside the hub.

(Herrera et al., 2021)

The expression of Dp^GD4444 under the control of Scer\GAL4^Mef2.PR leads to small ventral longitudinal muscles 3 in third instar larvae.

The expression of Dp^GD4444 under the control of Scer\GAL4^Mef2.PR, Scer\GAL4^Cg.PA or Scer\GAL4^apolpp.PB leads to binucleated cells and smaller lipid droplets in the third instar larval fat body.

(Zappia et al., 2021)

Expression of Dp^GD4444 under the control of Scer\GAL4^Bx-MS1096 results in male flies with weak wing size and patterning defects.

Expression of Dp^GD4444 under the control of Scer\GAL4^Bx-MS1096 results in female flies with weak wing size and patterning defects.

(Schertel et al., 2015)

Adults expressing Dp^GD4444 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 30-40% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 70-80% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4444

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dp^GD4444, Scer\GAL4^NP1624, Scer\GAL80^{hh-MI10526-T3XG80.0} has decreased cell number | spermatogenesis phenotype, suppressible by Fs^UAS.cPa, Scer\GAL4^NP1624, Scer\GAL80^{hh-MI10526-T3XG80.0}

(Herrera et al., 2021)

Suppressor of

Statement

Reference

Dp^GD4444, Scer\GAL4^NP1624 is a suppressor of abnormal cell number | somatic clone | spermatogenesis phenotype of Rbf^HMS03004, Scer\GAL4^NP1624

(Sainz de la Maza et al., 2022)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dp^GD4444, Scer\GAL4^NP1624, Scer\GAL80^{hh-MI10526-T3XG80.0} has hub cell | decreased number phenotype, suppressible by Fs^UAS.cPa, Scer\GAL4^NP1624, Scer\GAL80^{hh-MI10526-T3XG80.0}

(Herrera et al., 2021)

Suppressor of

Statement

Reference

Dp^GD4444, Scer\GAL4^NP1624 is a suppressor of somatic cell of testis | somatic clone phenotype of Rbf^HMS03004, Scer\GAL4^NP1624

(Sainz de la Maza et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Dp^GD4444 is partially rescued by Dp^UAS.cDa/Scer\GAL4^NP1624

(Herrera et al., 2021)

Dp^GD4444 is partially rescued by Scer\GAL80^{hh-MI10526-T3XG80.0}/Dp^UAS.cDa/Scer\GAL4^NP1624

(Herrera et al., 2021)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

v12722

w¹¹¹⁸; P{GD4444}v12722

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4444

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Dp^GD4444

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections