FlyBase Allele Report: Dmel\botv[GD2083]

General Information

Symbol

Dmel\botv^GD2083

Species

D. melanogaster

Name

FlyBase ID

FBal0209171

Feature type

allele

Associated gene

Dmel\botv

Associated Insertion(s)

Carried in Construct

P{GD2083}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2083}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2083

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neonatal diabetes

modeled by Hsap\INS^C96Y.UAS

(He et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut epithelium, with Scer\GAL4^esg.PU

(Ma et al., 2019)

adult posterior midgut epithelium, with Scer\GAL4^esg.PU

(Ma et al., 2019)

dendritic tree | cell non-autonomous | third instar larval stage, with Scer\GAL4^hh-Gal4

(Poe et al., 2017)

distal dendrite | cell non-autonomous | third instar larval stage, with Scer\GAL4^hh-Gal4

(Poe et al., 2017)

enteroblast of posterior adult midgut epithelium | increased number, with Scer\GAL4^esg.PU

(Ma et al., 2019)

enterocyte of posterior adult midgut epithelium, with Scer\GAL4^esg.PU

(Ma et al., 2019)

intestinal stem cell of posterior adult midgut epithelium | increased number, with Scer\GAL4^esg.PU

(Ma et al., 2019)

larval multidendritic class IV neuron | cell non-autonomous | third instar larval stage, with Scer\GAL4^hh-Gal4

(Poe et al., 2017)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Adulthood-only expression of botv^GD2083 under the combined control of Scer\GAL4^esg.PU and Gal80[ts] (induced by temperature shift from 18[o]C to 29[o]C) leads to adult posterior midguts showing increased mitotic index (pH3 staining), and increased numbers of stem cells and enteroblasts (i.e. esg-, Dl- and GBE+Su(H)-positive cells), but unchanged numbers of enteroendocrine cells (Pros-positive cells), as compared to controls; some of the progenitor cells (esg-positive) mis-express the enterocyte marker PDM1, indicative of intestinal homeostasis loss; the size of the polyploid cells expressing botv^GD2083 is smaller than fully differentiated enterocytes, indicating a defect in maturation.

(Ma et al., 2019)

Expression of botv^GD2083 in epidermic cells under the control of Scer\GAL4^hh-Gal4 results in severe reduction in the overall dendrite or terminal dendrite density as well as terminal dendrite number within the intersegmental area in the adjacent class IV dendritic arborizing neurons in third instar larvae.

(Poe et al., 2017)

Adults expressing botv^GD2083 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 50-60% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development