FlyBase Allele Report: Dmel\dpp[UAS.cUa]

General Information

Symbol

Dmel\dpp^UAS.cUa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Unknown

FlyBase ID

FBal0221546

Feature type

allele

Associated gene

Dmel\dpp

Associated Insertion(s)

Carried in Construct

P{UAS-dpp.U}

Also Known As

UAS-dpp

Key Links

Transgenic product class

wild_type

(Singh et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Singh et al., 2006)

Mutagen

in vitro construct

(Singh et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-dpp.U}

Cytology

Description

UAS regulatory sequences drive expression of dpp.

(Singh et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dpp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | embryonic stage, with Scer\GAL4^69B

(Yang and Su, 2011)

abnormal cell number | embryonic stage, with Scer\GAL4^how-24B

(Yang and Su, 2011)

decreased cell number | progressive, with Scer\GAL4^hh.PU

(Pan et al., 2007)

increased cell death | embryonic stage, with Scer\GAL4^69B

(Yang and Su, 2011)

increased cell death | embryonic stage, with Scer\GAL4^Act5C.PU

(Yang and Su, 2011)

increased cell death | embryonic stage, with Scer\GAL4^how-24B

(Yang and Su, 2011)

lethal, with Scer\GAL4^bab1-Gal4

(Bolivar et al., 2006)

visible, with Scer\GAL4^ey.PU

(Salzer et al., 2010)

Phenotype Manifest In

embryo | dorsal, with Scer\GAL4^69B

(Yang and Su, 2011)

embryonic cardioblast, with Scer\GAL4^69B

(Yang and Su, 2011)

embryonic cardioblast, with Scer\GAL4^how-24B

(Yang and Su, 2011)

embryonic myoblast, with Scer\GAL4^69B

(Yang and Su, 2011)

embryonic pericardial cell, with Scer\GAL4^69B

(Yang and Su, 2011)

embryonic pericardial cell, with Scer\GAL4^how-24B

(Yang and Su, 2011)

eye, with Scer\GAL4^ey.PU

(Salzer et al., 2010)

female germline stem cell | increased number | temperature conditional, with Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B

(Bolivar et al., 2006)

female germline stem cell | progressive, with Scer\GAL4^hh.PU

(Pan et al., 2007)

germarium | progressive, with Scer\GAL4^hh.PU

(Pan et al., 2007)

germarium cap cell | progressive, with Scer\GAL4^hh.PU

(Pan et al., 2007)

male germline stem cell | increased number, with Scer\GAL4^nanos.PG

(Singh et al., 2006)

ventral denticle belt | absent | embryonic stage, with Scer\GAL4^69B

(Yang and Su, 2011)

Detailed Description

Statement

Reference

Adulthood-only expression of dpp^UAS.cUa under the combined control of Scer\GAL4^esg.PU and Gal80[ts] (induced by temperature shift from 18[o]C to 29[o]C) does not affect the number of intestinal progenitors (i.e. esg-positive cells) in the adult posterior midgut, as compared to controls.

(Ma et al., 2019)

Forced expression of dpp^Scer\UAS.cUa driven by Scer\GAL4^ey.PU appears to initiate ectopic eye formation at the anterior margin of the eye field. Often the result is the production of two compound eyes being generated from a single retinal field.

(Salzer et al., 2010)

63-day old germaria expressing dpp^Scer\UAS.cUa via Scer\GAL4^hh.PU (with expression controlled via 'UAS-gal80[ts]' and temperature) have significantly fewer germline cell stems and cap cells compared to controls.

(Pan et al., 2007)

Females in which expression of dpp^Scer\UAS.cUa under the control of Scer\GAL4^bab1-Gal4 has been restricted to the adult stage using Scer\GAL80^ts.αTub84B (animals are shifted to the restrictive temperature of 31[o]C when adult) show a strong germline stem cell tumour phenotype in the ovary after 3 days of dpp^Scer\UAS.cUa expression.

(Bolivar et al., 2006)

Expression of dpp^Scer\UAS.cUa under the control of Scer\GAL4^nos.PG results in ectopic germline stem cells in the testis.

(Singh et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PU, dpp^UAS.cUa has increased cell death | embryonic stage phenotype, suppressible by Scer\GAL4^Act5C.PU/Tak1^K46R.UAS

(Yang and Su, 2011)

Scer\GAL4^Act5C.PU, dpp^UAS.cUa has increased cell death | embryonic stage phenotype, suppressible by Scer\GAL4^Act5C.PU/bsk^DN.UAS.cUa

(Yang and Su, 2011)

Scer\GAL4^Act5C.PU, dpp^UAS.cUa has increased cell death | embryonic stage phenotype, suppressible by Scer\GAL4^Act5C.PU/p53^DN.UAS.cUa

(Yang and Su, 2011)

Scer\GAL4^Act5C.PU, dpp^UAS.cUa has increased cell death | embryonic stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PU

(Yang and Su, 2011)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Act5C.PU/dpp^UAS.cUa is a non-enhancer of decreased cell death | embryonic stage phenotype of Scer\GAL4^Act5C.PU, Tak1^K46R.UAS

(Yang and Su, 2011)

Suppressor of

Statement

Reference

dpp^UAS.cUa/Scer\GAL4^esg.PU is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4^esg.PU, botv^GD2083

(Ma et al., 2019)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Act5C.PU/dpp^UAS.cUa is a non-suppressor of decreased cell death | embryonic stage phenotype of Scer\GAL4^Act5C.PU, Tak1^K46R.UAS

(Yang and Su, 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^nanos.PG, dpp^UAS.cUa has male germline stem cell | increased number phenotype, suppressible by BHD^RNAi.Sym.UAS, Scer\GAL4^nanos.PG

(Singh et al., 2006)

Suppressor of

Statement

Reference

dpp^UAS.cUa/Scer\GAL4^esg.PU is a suppressor of adult posterior midgut epithelium phenotype of Scer\GAL4^esg.PU, sfl^HMS00543

(Ma et al., 2019)

Scer\GAL4^nanos.PG/dpp^UAS.cUa is a suppressor | partially of spermatogonium | heat sensitive phenotype of Stat92E^F/Stat92E^j6C8

(Singh et al., 2006)

Scer\GAL4^nanos.PG/dpp^UAS.cUa is a suppressor | partially of male germline stem cell | heat sensitive phenotype of Stat92E^F/Stat92E^j6C8

(Singh et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of dpp^Scer\UAS.cUa under the control of Scer\GAL4^nos.PG significantly slows down the loss of germ cell phenotype that is seen in Stat92E^F/Stat92E^j6C8 testes at 29[o]C. After 4 days at 29[o]C, 41% of the double mutant testes contain one or more germline stem cells and all of the testes retain spermatogonia. After 7 days at 29[o]C, 17% of the double mutant testes have one or more germline stem cells and 88% retain spermatogonia.

Co-expression of BHD^{dsRNA.Sym.Scer\UAS} significantly suppresses the male germline stem cell expansion phenotype caused by expression of dpp^Scer\UAS.cUa under the control of Scer\GAL4^nos.PG.

(Singh et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dpp^Scer\UAS.cUa

dpp^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

References (25)

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