FlyBase Allele Report: Dmel\cas[GD1398]

General Information

Symbol

Dmel\cas^GD1398

Species

D. melanogaster

Name

FlyBase ID

FBal0209226

Feature type

allele

Associated gene

Dmel\cas

Associated Insertion(s)

Carried in Construct

P{GD1398}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1398}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1398

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal, with Scer\GAL4^Act5C.PU

(Dietzl et al., 2007)

lethal | male, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

macrochaeta, with Scer\GAL4^pnr-MD237

(Dietzl et al., 2007)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Adults expressing cas^GD1398 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in a weak flyer phenotype.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 70-80% or 80-90% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Expression of cas^GD1398, under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF, leads to a mild rough eye phenotype.

Expression of cas^GD1398, under the control of Scer\GAL4^pnr-MD237, leads to missing macrochaetae, although this phenotype is mild.

(Dietzl et al., 2007)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1398

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH, cas^GD1398 has lethal | female phenotype

(Dietzl et al., 2007)

Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH, cas^GD1398 has abnormal eye color phenotype

(Dietzl et al., 2007)

Dcr-2^UAS.cDa, Scer\GAL4^pnr-MD237, cas^GD1398 has lethal phenotype

(Dietzl et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH, cas^GD1398 has eye phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of Dcr-2^Scer\UAS.cDa with cas^GD1398, under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF, leads to an enhancement of the cas^GD1398 eye phenotype, with eyes displaying rough, glazed and eye color phenotypes. Additionally, coexpression of Dcr-2^Scer\UAS.cDa and cas^GD1398 with the same drivers leads to lethality in both genders while expression of cas^GD1398 only leads to male lethality.

Coexpression of Dcr-2^Scer\UAS.cDa and cas^GD1398, under the control of Scer\GAL4^pnr-MD237, results in lethality, a phenotype not seen when cas^GD1398 alone is expressed with this driver.

(Dietzl et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v2929

w¹¹¹⁸; P{GD1398}v2929

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1398

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

cas^GD1398

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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