FlyBase Allele Report: Dmel\ed[GD2575]

General Information

Symbol

Dmel\ed^GD2575

Species

D. melanogaster

Name

FlyBase ID

FBal0209497

Feature type

allele

Associated gene

Dmel\ed

Associated Insertion(s)

Carried in Construct

P{GD2575}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2575}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2575

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion | cell autonomous | larval stage

(Chang et al., 2011)

abnormal cell adhesion | cell autonomous | larval stage, with Scer\GAL80^ts.αTub84B

(Chang et al., 2011)

abnormal cell shape | cell autonomous | larval stage | somatic clone

(Chang et al., 2011)

abnormal cell shape | cell autonomous | larval stage | somatic clone, with Scer\GAL80^ts.αTub84B

(Chang et al., 2011)

abnormal size | adult stage, with Scer\GAL4^ey.PH

(Yue et al., 2012)

abnormal size | adult stage, with Scer\GAL4^hh-Gal4

(Yue et al., 2012)

increased cell size | larval stage, with Scer\GAL4^hth-GETDB

(Ku and Sun, 2017)

lethal, with Scer\GAL4^Act5C.PU

(Dietzl et al., 2007)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^ey.PH

(Yue et al., 2012)

visible | adult stage, with Scer\GAL4^hh-Gal4

(Yue et al., 2012)

Phenotype Manifest In

antennal disc | larval stage, with Scer\GAL4^hth-GETDB

(Ku and Sun, 2017)

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

eye, with Scer\GAL4^ey.PH

(Yue et al., 2012)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^hh-Gal4

(Yue et al., 2012)

wing disc | cell autonomous | somatic clone

(Chang et al., 2011)

wing disc | cell autonomous | somatic clone, with Scer\GAL80^ts.αTub84B

(Chang et al., 2011)

wing disc | cell non-autonomous | somatic clone

(Chang et al., 2011)

wing disc | cell non-autonomous | somatic clone, with Scer\GAL80^ts.αTub84B

(Chang et al., 2011)

Detailed Description

Statement

Reference

Expression of ed^GD2575 under the control of Scer\GAL4^hth-GETDB in larval antennal discs leads to some cells showing morphological defects, such as swelling, enlargement, or delamination, as compared to controls.

(Ku and Sun, 2017)

No discernible eye overgrowth is seen when ed^GD2575 is expressed under the control of Scer\GAL4^GMR.PF. However, expressing ed^GD2575 under the control of Scer\GAL4^ey.PH does result in eye overgrowth.

Expression of ed^GD2575 under the control of Scer\GAL4^hh-Gal4 produces adult wings with enlarged posterior compartments.

(Yue et al., 2012)

Larval wing disc clones expressing ed^GD2575 sort out from the surrounding wild type cells, exhibiting both rounded and smooth contours rather than the jagged border seen in controls. The wild type cells surrounding the clone assemble a ring of actomyosin cable at the interface and the clone cells themselves develop an apical constriction that is inversely proportional to the size of the clone. shg levels are reduced at some clonal interfaces.

Limiting expression of ed^GD2575 to a 10 hour period using Scer\GAL80^ts.αTub84B results in clones with a jagged boundary, as is seen in wild type controls. Clone cells do not display any apical construction and are not surrounded by actomyosin cable. Normal levels of shg are also seen. After 12 hours at the restrictive temperature, the shape of the clone varies depending on the level of ed expression, with the level of ed correlating with the amount of actomyosin cable seen. Lower ed levels are also associated with a smoother interface and weaker apical constriction in the clone cells. shg levels remain unchanged. By 22 hours at the restrictive temperature completely ed expression is completely restricted and the clones appear increasingly smooth. A thick actin cable surrounds the entire interface and clear apical constriction is seen in the clone cells. shg disappears from the cell interface.

(Chang et al., 2011)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 50-60% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 50-60% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2575

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ed^GD2575/Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PF, yki^UAS.cZa

(Yue et al., 2012)

ed^GD2575/Scer\GAL4^GMR.PF is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PF, yki^UAS.cZa

(Yue et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

ed^GD2575/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, yki^UAS.cZa

(Yue et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ed^GD2575 enhances the eye overgrowth seen when yki^Scer\UAS.cZa is expressed under the control of Scer\GAL4^GMR.PF.

(Yue et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2575

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ed^GD2575

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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