FlyBase Allele Report: Dmel\enok[GD4037]

General Information

Symbol

Dmel\enok^GD4037

Species

D. melanogaster

Name

FlyBase ID

FBal0209521

Feature type

allele

Associated gene

Dmel\enok

Associated Insertion(s)

Carried in Construct

P{GD4037}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4037}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4037

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Combined expression of RNAi transgenes enok^GD4037 and enok^HM05195 under the control of Scer\GAL4^bab1-Gal4 results in a significant decrease of female germline stem cell (GSC) numbers per germarium during a two week period after eclosion. Expression of the RNAi lines only at adulthood in temperature shift experiments using Scer\GAL80^ts.αTub84B causes a similar gradual GSC loss.

Expression of Scer\GAL4^bab1-Gal4>enok^GD4037 results in a progressive decline in germarium cap cell numbers compared with wild-type.

(Xin et al., 2013)

Adults expressing enok^GD4037 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies or early pupal lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in frayed myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4037

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B, enok^GD4037 has germarium cap cell | progressive | heat sensitive phenotype, suppressible by Delta::N^ΔECN.UAS, Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B

(Xin et al., 2013)

Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B, enok^GD4037 has female germline stem cell | progressive | heat sensitive phenotype, suppressible | partially by Delta::N^ΔECN.UAS, Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B

(Xin et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B, enok^GD4037, enok^HM05195 has female germline stem cell | progressive | heat sensitive phenotype, non-suppressible by dpp^UAS.cNa/Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B

(Xin et al., 2013)

Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B, enok^GD4037, enok^HM05195 has female germline stem cell | progressive | heat sensitive phenotype, non-suppressible by dally^UAS.cJa/Scer\GAL4^bab1-Gal4, Scer\GAL80^ts.αTub84B

(Xin et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of dpp^Scer\UAS.cNa with enok^GD4037 and enok^HM05195 under the control of Scer\GAL4^bab1-Gal4 and Scer\GAL80^ts.αTub84B is unable to attenuate the decline in germline stem cell numbers.

Co-expression of dally^Scer\UAS.cJa with enok^GD4037 and enok^HM05195 under the control of Scer\GAL4^bab1-Gal4 and Scer\GAL80^ts.αTub84B is unable to attenuate the decline in germline stem cell numbers.

The germarium cap cell loss resulting from the expression of enok^GD4037 can be fully rescued by forced expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^bab1-Gal4 and Scer\GAL80^ts.αTub84B.

The germline stem cell loss resulting from the expression of enok^GD4037 can be partially rescued by forced expression of Dl::N^{ΔECN.Scer\UAS} under the control of Scer\GAL4^bab1-Gal4 and Scer\GAL80^ts.αTub84B.

(Xin et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v37526

w¹¹¹⁸; P{GD4037}v37526

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4037

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

enok^GD4037

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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