FlyBase Allele Report: Dmel\kirre[GD14476]

General Information

Symbol

Dmel\kirre^GD14476

Species

D. melanogaster

Name

FlyBase ID

FBal0209786

Feature type

allele

Associated gene

Dmel\kirre

Associated Insertion(s)

Carried in Construct

P{GD14476}

Also Known As

UAS-kirre-RNAi, UAS-kirre RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14476}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14476

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of kidney disease

CEA

(Zhang et al., 2013)

model of nephrotic syndrome

CEA

(Koehler et al., 2020, Zhuang et al., 2009)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Act5C.PU

(Dietzl et al., 2007)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

embryonic/larval garland cell, with Scer\GAL4^sns.GCN

(Zhuang et al., 2009)

embryonic/larval pericardial cell, with Scer\GAL4^Ugt36A1.PK

(Zhang et al., 2013)

Detailed Description

Statement

Reference

Scer\GAL4^Dot.PK-mediated expression of kirre^GD14476 completely abolishes the normal accumulation of ANF-RFP (expressed via Rnor\ANF^{Mhc.T:Disc\RFP}) in pericardial nephrocytes.

(Zhang et al., 2013)

Adults expressing kirre^GD14476 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

The garland cell nephrocytes of third instar larvae expressing kirre^GD14476 under the control of Scer\GAL4^sns.GCN have shape defects and are more loosely associated than normal.

The numbers of nephrocyte diaphragms in the garland cell nephrocytes of second instar larvae expressing kirre^GD14476 under the control of Scer\GAL4^sns.GCN is reduced compared to controls, although the NDs that do form appear normal by TEM analysis.

The surface of the garland cell nephrocytes of third instar larvae expressing kirre^GD14476 under the control of Scer\GAL4^sns.GCN is more uniform than that of control cells when analysed by SEM.

Garland cell nephrocytes from second instar larvae expressing kirre^GD14476 under the control of Scer\GAL4^sns.GCN show reduced uptake of both dextran 10kD and Alexa Fluor 555 BSA compared to controls after co-incubation with both tracers for 30 seconds.

Garland cell nephrocytes from second instar larvae expressing kirre^GD14476 under the control of Scer\GAL4^sns.GCN show efficient uptake of dextran 10kD after co-incubation with both Cascade Blue dextran 10kD and fluorescein dextran 500kD for 5 minutes. However, these cells show a very poor uptake of dextran 500kD under these conditions compared to controls.

(Zhuang et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development